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Agaricus bisporus liquid strain and preparation method thereof

A technology for liquid strains and Agaricus bisporus, which is applied in the fields of botanical equipment and methods, horticulture, and application, can solve the problems of low preparation success rate, affecting the cultivation quality and yield of Agaricus bisporus, aging and degeneration of mycelium, etc.

Inactive Publication Date: 2016-04-27
CHAOYANG XINYUAN AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the Agaricus bisporus liquid culture medium still has problems such as low preparation success rate, unsuitable mycelia growth, easy mycelium aging and degeneration in the later stage of cultivation, and high professional technical requirements for operation. In the process of large-scale production, it will Directly affect the cultivation quality and yield of Agaricus bisporus

Method used

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  • Agaricus bisporus liquid strain and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. The tested strain: Shandong Shouguang Agaricus bisporus parent species 2796;

[0024] 2. Culture medium formula

[0025] a) Slant medium: 200 g of potatoes, 150 g of cow dung, 20 g of glucose, 2 g of potassium dihydrogen phosphate, 1 g of magnesium sulfate, 2 g of peptone, 20 g of agar, natural pH, and 1000 ml of water.

[0026] b) Shake flask medium: 200g potato, 150g cow dung, 10g corn flour, 10g bran, 20g glucose, 2g potassium dihydrogen phosphate, 1g magnesium sulfate, 2g peptone, vitamin B 1 20mg, PH natural, water 1000ml.

[0027] c) Fermenter medium (prepared according to 50 liters): 2.5kg of potatoes, 1kg of cow dung, 0.5kg of bran, 0.25kg of corn flour, 0.5kg of sucrose, 50g of potassium dihydrogen phosphate, 25g of magnesium sulfate, vitamins B1 30 mg, 50 g of defoamer, natural pH, 50 liters of water.

[0028] Cow dung treatment: Cow dung is dried, pulverized, sieved, exposed to the sun and dried for later use.

[0029] 3. Activation of strains: insert ...

Embodiment 2

[0044] 1. Culture medium formula

[0045] a) Slant medium: 150 g of potatoes, 100 g of cow dung, 15 g of glucose, 1.5 g of potassium dihydrogen phosphate, 0.8 g of magnesium sulfate, 1.5 g of peptone, 15 g of agar, natural pH, and 1000 ml of water.

[0046] b) Shake flask medium: 150g potato, 100g cow dung, 8g corn flour, 8g bran, 15g glucose, 1.5g potassium dihydrogen phosphate, 0.8g magnesium sulfate, 1.5 peptone, vitamin B 1 15mg, PH natural, water 1000ml.

[0047] c) Fermenter culture medium (prepared according to 50 liters): 2.0kg of potatoes, 1kg of cow dung, 0.5kg of bran, 0.2kg of corn flour, 0.5kg of sucrose, 40g of potassium dihydrogen phosphate, 20g of magnesium sulfate, vitamins B1 20 mg, 40 g of defoamer, natural pH, 50 liters of water.

[0048] 2. The preparation method of Agaricus bisporus liquid strain is the same as in Example 1.

Embodiment 3

[0050] 1. Culture medium formula

[0051] a) Slant medium: 200 g of potatoes, 120 g of cow dung, 18 g of glucose, 1.8 g of potassium dihydrogen phosphate, 0.8 g of magnesium sulfate, 2 g of peptone, 25 g of agar, natural pH, and 1000 ml of water.

[0052] b) Shake flask culture medium: 200g potato, 120g cow dung, 15g corn flour, 15g bran, 20g glucose, 1.8g potassium dihydrogen phosphate, 0.8g magnesium sulfate, 2 peptone, vitamin B 1 20mg, PH natural, water 1000ml.

[0053] c) Fermenter culture medium (prepared according to 50 liters): 2.5kg of potatoes, 2.5kg of cow dung, 1.0kg of bran, 0.3kg of corn flour, 0.5kg of sucrose, 55g of potassium dihydrogen phosphate, 25g of magnesium sulfate, vitamins B1 30 mg, 50 g of defoamer, natural pH, 50 liters of water.

[0054] 2. The preparation method of Agaricus bisporus liquid strain is the same as in Example 1.

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Abstract

The invention discloses an Agaricus Bisporus liquid spawn and a preparation method thereof. The Agaricus Bisporus liquid spawn is produced by means of three-level propagation through slant and shake-flask media and a fermenter medium. The slant and shake-flask media comprise components including 100-200g of potato, 50-150g of cow dung, 10-30g of glucose, 1-2.5g of mono potassium phosphate, 0.5-2g of magnesium sulfate and 1-4g of peptone; the fermenter medium of every 50 liters of water is composed of 2-4kg of potato, 1-3kg of cow dung, 0.5-1kg of bran, 0.2-0.4 kg of corn powder, 0.5-1kg of sucrose, 30-60g of mono potassium phosphate, 20-30g of magnesium sulfate, 120-150 mg of vitamin B and 40-50g of defoamer. By application of the Agaricus Bisporus liquid spawn and the preparation method thereof, the problem that mycelia are prone to aging and degradation during growth is solved; the liquid spawn is of globular mycelia with uniform age, high activity and fast running; a high-running spawn center is formed after the liquid spawn is inoculated to solid media, with uniform fruiting achieved; the preparation cost is only one-tenth of that of solid spawn; the growth cycle of fermenter spawn is 7-9 days, and thus spawns on a large scale can be cultured within a short time.

Description

technical field [0001] The invention belongs to the technical field of liquid strains of edible fungi, and in particular relates to liquid strains of Agaricus bisporus among edible fungi and a preparation method of the liquid strains. Background technique [0002] The strains used in the production of Agaricus bisporus include solid strains and liquid strains. At present, the edible fungus Agaricus bisporus usually adopts the traditional solid strain cultivation mode, and the cultivation of Agaricus bisporus strains mostly uses wheat grains as raw materials. The second-level solid strains are made by connecting the first-level PDA test tube slanted parent species to the wheat grain medium, and then Propagation of secondary species to make tertiary solid strains has disadvantages such as long production cycle, high production cost, high pollution rate, long bacterial age, irregular and inconsistent fruiting in actual production. It generally takes 35-40 days for solid strain...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
Inventor 于海超辛子军徐晓东张福艳王妍曲明福许国兴刘建国张慧颖徐箭李英杰鞠植珍于虎元阚秋芳
Owner CHAOYANG XINYUAN AGRI DEV
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