Preparation method and application of Armillaria luteo-virens antioxidant active component

An antioxidant activity, Armillaria yellow-green technology, applied in the application, food preparation, antitoxin and other directions, can solve the problems of inability to guarantee consistency, high reagent consumption, non-repeatability, etc., to save extraction cost and Time cost, small reagent consumption, and the effect of improving extraction rate

Active Publication Date: 2014-05-14
贵州贵安精准医学股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following defects: (1) The traditional solvent extraction method (liquid-liquid extraction) is used to extract effective active components, and there is no repeatability between batches, that is, it is impossible to guarantee that the material components obtained by the first batch of extraction are consistent with The consistency between the substance component

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  • Preparation method and application of Armillaria luteo-virens antioxidant active component

Examples

Experimental program
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Embodiment 1

[0036] Example 1: Extraction of Antioxidant Active Components of Armillaria Chrysanthemum Fruiting Body

[0037] The preparation method of the antioxidation active component of Armillaria chrysanthemum fruiting body of this embodiment comprises the following steps:

[0038] (1) Armillaria chrysanthemum fruiting bodies were dried in the shade at room temperature, dried and dehydrated at 60°C, and then ultrafinely pulverized at -20°C for 5 minutes to obtain superfine powder of Armillaria chrysanthemum fruiting bodies.

[0039] (2) Take 1Kg of the above-mentioned superfine powder of Armillaria chrysanthemum fruiting body, add 6.0L of analytical pure water to extract 3 times at 90°C, each time for 1h, and centrifuge at 8000rpm to collect the supernatant. The supernatant is concentrated to a solid content of 50 mg / mL, and 4 times the volume of absolute ethanol is added for alcohol precipitation treatment, and the supernatant is collected by centrifugation, and the alcohol precipita...

Embodiment 2

[0044] Example 2: Extraction of Antioxidant Active Components of Armillaria Chrysanthemum Fruiting Body

[0045] The preparation method of the antioxidation active component of Armillaria chrysanthemum fruiting body of this embodiment comprises the following steps:

[0046] (1) Armillaria chrysanthemum fruiting bodies were dried in the shade at room temperature, dried and dehydrated at 40°C, and then ultrafinely pulverized at 4°C for 20 minutes to obtain superfine powder of Armillaria chrysanthemum fruiting bodies.

[0047] (2) Take 0.3Kg of the above-mentioned superfine powder of Armillaria chrysanthemum fruiting bodies, add 4.5L of analytically pure water to extract 3 times at 60°C, each time for 2h, and centrifuge at 4000rpm to collect the supernatant. The supernatant is concentrated to a solid content of 200 mg / mL, and 9 times the volume of absolute ethanol is added for alcohol precipitation treatment, and the supernatant is collected by centrifugation, and the alcohol pre...

Embodiment 3

[0052] Example 3: Extraction of Antioxidant Active Components of Armillaria Chrysanthemum Fruiting Body

[0053] The preparation method of the antioxidation active component of Armillaria chrysanthemum fruiting body of this embodiment comprises the following steps:

[0054] (1) Armillaria chrysanthemum fruiting bodies were dried in the shade at room temperature, dried and dehydrated at 50°C, and then ultrafinely pulverized at -10°C for 10 minutes to obtain Armillaria chrysanthemum fruiting body superfine powder.

[0055] (2) Take 0.5Kg of the above-mentioned superfine powder of Armillaria chrysanthemum fruiting bodies, add 5L of analytically pure water to extract twice at 75°C, each time for 2h, and centrifuge at 5000rpm to collect the supernatant. The supernatant is concentrated to a solid content of 100mg / mL, and 6 times the volume of absolute ethanol is added for alcohol precipitation treatment, and the supernatant is collected by centrifugation, and the alcohol precipitati...

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Abstract

The invention provides a preparation method of an Armillaria luteo-virens antioxidant active component, which includes the following steps: superfine-grinding Armillaria luteo-virens fruiting body to obtain superfine powder; extracting with water for many times, collecting supernatant, carrying out alcohol precipitation on the supernatant with anhydrous ethanol, and concentrating the supernatant subjected to alcohol precipitation to obtain a low-molecular-weight extract; dissolving the low-molecular-weight extract with analytically pure water, filtering with an organic filter membrane, and performing solid phase extraction on the sample solution with C18 or C8 preparative chromatography to obtain a pretreated sample; separating and purifying the pretreated sample with C18 preparative chromatography, and collecting according to chromatogram peaks to obtain various test components; and carrying out antioxidant activity tests on the test components, and selecting the component with the best antioxidant activity as the Armillaria luteo-virens antioxidant active component. The method provided by the invention has the advantages of simple and rapid process, easiness in implementation, high efficiency, good repeatability and good stability and controllability; the obtained Armillaria luteo-virens antioxidant active component can be used for preparing antioxidant medicaments or health foods.

Description

technical field [0001] The invention relates to the technical fields of medicinal chemistry and biomedicine, in particular to a preparation method of an Armillaria chrysanthemum antioxidant active component and the application of the active component in the preparation of antioxidant drugs or health food. Background technique [0002] Armillaria chrysanthemum, commonly known as chanterelle, is a rare edible and medicinal fungus unique to the Qinghai-Tibet Plateau. Wild Armillaria chrysanthemum fruiting bodies are rich in protein, amino acids, vitamins, minerals, alkaloids, volatile oils and polysaccharides, and also contain a small amount of organic acids, flavonoids, cardiac glycosides, volatile oils, steroidal triterpenoids, Glycosides, coumarin, terpenoids, tannins, phenolic compounds, etc., especially the high content of selenium, are the killer of cancer. Studies have shown that Armillaria chrysanthemum has biological activities such as preventing and treating neuritis...

Claims

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Application Information

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IPC IPC(8): A61K36/06A23L1/29A23L1/28A61P39/06A23L31/00
Inventor 张耀洲党军陶燕铎马琳崔今松
Owner 贵州贵安精准医学股份有限公司
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