Preparation method of medicinal plant extract for reducing blood sugar and application in pharmacy and health food thereof
A technology of medicinal plants and extracts, applied in the preparation of medicinal plants-wild pineapple hypoglycemic extract and its active parts, in the development of pharmaceuticals and health care products, and can solve problems such as inability to achieve a radical cure
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Embodiment 1
[0025] Example 1 Preparation of medicinal plant extracts and effective parts thereof in the present invention (1)
[0026] Take fresh and ripe wild pineapple, put it in a container, add 10-20 times of water, soak for 2 hours, decoct 3 times for 2 hours each time, filter, concentrate until dry to obtain the medicinal plant extract. After the medicinal plant extract is dissolved in water, it is separated by D-101 macroporous adsorption resin, eluted with different concentrations of ethanol-water solution until it is colorless, and the eluate is concentrated under reduced pressure to obtain the water eluate, 70% The ethanol eluate is concentrated and spray-dried to obtain the effective fraction of the present invention.
Embodiment 2
[0027] Example 2 Preparation of medicinal plant extracts and effective parts thereof in the present invention (two)
[0028] Take the freshly matured wild pineapple which has been dried in the shade, put it in a container, add 10-20 times of ethanol or ethanol-water (7:3), soak for 2 hours, reflux extraction for 3 times, each time for 2 hours, filter, and concentrate until dry. Medicinal plant extracts. After the medicinal plant extract is dissolved in water, degreased by the organic solvent chloroform or ethyl acetate, it is repeatedly extracted with n-butanol until it is colorless, the extract is concentrated under reduced pressure, and spray-dried to obtain the effective part of the present invention .
Embodiment 3
[0029] Embodiment 3 The influence of the medicinal plant extract of the present invention on the insulin tolerance of spontaneous diabetic mice
[0030] 3.1. Test materials: wild pineapple water extract, metformin, high-fat feed, Roche automatic blood glucose meter
[0031] 3.2. Test animals: db / db spontaneous diabetic mice (30-40g)
[0032] 3.3. Test method: Take 60 male db / db spontaneous diabetic mice of 30-40g, and randomly divide them into three dosage groups of hyperglycemia model group, metformin positive control group and wild pineapple water extract (doses are respectively 50mg / kg, 100mg / kg, 200mg / kg), 100mg / kg metformin group, a total of 5 groups (12 rats), all were fed high-fat feed, and the corresponding drugs were given by intragastric administration at the same time, and the hyperglycemia model group was intragastrically administered the same volume of tap water. After 3 weeks of administration, the mice in each group were fasted for 4 hours, and 0.8 U / kg insulin...
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