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Method for improving demulsification performance of cell wall combined biological de-emulsifier

A biological demulsifier and combined technology, applied in the field of environmental microorganisms, can solve problems that do not involve the performance optimization of downstream biological demulsifiers, and achieve the effects of easy large-scale industrial application, simple operation and low cost

Inactive Publication Date: 2015-06-03
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Both of the above two patents focus on the extraction of cell wall-bound biodemulsifiers, neither involving the improvement of its production in the upstream culture stage, nor the performance optimization of downstream biodemulsifiers

Method used

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  • Method for improving demulsification performance of cell wall combined biological de-emulsifier
  • Method for improving demulsification performance of cell wall combined biological de-emulsifier
  • Method for improving demulsification performance of cell wall combined biological de-emulsifier

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Experimental program
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Effect test

Embodiment 1

[0036] 1. Add 0.5 g of cell wall-bound biodemulsifier dry powder produced by Alcaligenes sp.S-XJ-1 cultured with rapeseed oil as a carbon source into a 50 mL CH 3 OH and 0.42mL HCl in a Erlenmeyer flask.

[0037] 2. Place the Erlenmeyer flask containing the cell wall-bound biodemulsifier and reagents on a magnetic stirrer, add a magnetic stirrer, adjust the temperature to 25°C, set the speed to 500rpm, and stir for 24 hours.

[0038] 3. The obtained mixture was subjected to rotary evaporation, and the temperature of the rotary evaporation was set to 40°C. The time is 15-20min.

[0039] 4. Place the obtained biodemulsifier in an oven at a temperature of 80° C. to dry for 24 hours, and collect the obtained biodemulsifier.

[0040]5. Weigh a certain amount of biological demulsifier, dissolve it in water to make a 10g / L solution, take 2mL and add it to the demulsification tube, then add 18mL model emulsion, shake vigorously for 120 times, put it in a 35℃ water bath for 24 hours,...

Embodiment 2

[0043] 1. Add 0.5 g of cell wall-bound biodemulsifier produced by Alcaligenes sp.S-XJ-1 cultured with paraffin as a carbon source into 50 mL CH 3 OH and 0.42mL HCl in a Erlenmeyer flask.

[0044] 2. Place the Erlenmeyer flask containing the cell wall-bound biodemulsifier and reagents on a magnetic stirrer, add a magnetic stirrer, adjust the temperature to 25°C, set the speed to 500rpm, and stir for 24 hours.

[0045] 3. The obtained mixture was subjected to rotary evaporation, and the temperature of the rotary evaporation was set to 40°C. The time is 15-20min.

[0046] 4. Place the obtained biodemulsifier in an oven at a temperature of 80° C. to dry for 24 hours, and collect the obtained biodemulsifier.

[0047] 5. Weigh a certain amount of biological demulsifier, dissolve it in water to make a 10g / L solution, take 2mL and add it to the demulsification tube, then add 18mL model emulsion, shake vigorously for 120 times, put it in a 35℃ water bath for 24 hours, and observe the...

Embodiment 3

[0050] A method for improving the demulsification performance of a cell wall-bound biological demulsifier, the method comprising the following steps: freeze-drying the dry powder of the cell wall-bound biological demulsifier (generated by Alcaligenes sp.S-XJ-1) , to obtain dry powder of biological demulsifier, then mix and stir the dry powder of biological demulsifier with a certain proportion of methanol and hydrochloric acid mixed solution to carry out methyl esterification reaction, and finally, after rotary evaporation and drying, the cell wall-bound biological demulsifier with improved demulsification performance is obtained. Demulsifier.

[0051] The specific process of the methyl esterification reaction is as follows: the dry powder of the biological demulsifier is added to the mixed solution of methanol and hydrochloric acid with a volume ratio of 50:1, and the solid-liquid ratio of the dry powder of the biological demulsifier to the mixed solution is controlled at 0.00...

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Abstract

The invention relates to a method for improving a demulsification performance of a cell wall combined biological de-emulsifier. The method comprises the following steps: performing freeze drying on the cell wall combined biological de-emulsifier, so as to obtain dried biological de-emulsifier powder; mixing and stirring the dried biological de-emulsifier powder and a mixed solution of methanol and hydrochloric acid according to a certain ratio, carrying out methyl esterification reaction, and finally performing rotary evaporation and drying to obtain the cell wall combined biological de-emulsifier of which the demulsification performance is improved. Compared with the prior art, the method disclosed by the invention is simple and relatively low in cost, the demulsification performance of the cell wall combined biological de-emulsifier is obviously improved, and the large-scale application is easily realized.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, in particular to a method for improving the demulsification performance of a cell wall-bound biological demulsifier. Background technique [0002] Biodemulsifier is a kind of biosurfactant, which is a new type of demulsifier that can separate oil and water in emulsion. Compared with traditional chemical demulsifiers, biological demulsifiers have the advantages of high efficiency, low toxicity, biodegradability, and activity under extreme conditions, and have great application prospects in the fields of crude oil extraction and oily soil remediation. [0003] The biodemulsifier is produced by bacteria, and it is necessary to separate the biodemulsifier from the bacteria when extracting the biodemulsifier. [0004] At present, the bottleneck restricting the application of biological demulsifiers is mainly the high production cost. There are two ways to solve this problem: fir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D17/05
Inventor 黄翔峰陆丽君刘佳王凯彭开铭
Owner TONGJI UNIV
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