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Turbot hepcidin-based peptides and their applications

A technology of hepcidin and turbot, applied in the field of molecular biology, can solve the problems of unknown function and activity of hepcidin and anti-virus, and achieve the effect of improving the ability of anti-virus and anti-bacterial infection

Inactive Publication Date: 2016-01-20
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the functional activities of hepcidin encoded by these two genes (such as anti-virus, etc.) are unknown

Method used

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  • Turbot hepcidin-based peptides and their applications
  • Turbot hepcidin-based peptides and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] The hepcidin peptides SmHep1P and SmHep2P of the present invention are the amino acid sequences in SEQ ID No.1 and No.2 of the sequence table.

[0011] The sequence listing SEQIDNo.1 is:

[0012] QSHISLCRWCCNCCKANKGCGFCCKF

[0013] (a) Sequential features:

[0014] ●Length: 26

[0015] ●Type: amino acid sequence

[0016] ●Chain type: single chain

[0017] ●Topological structure: linear

[0018] (b) Molecule Type: Protein

[0019] (c) Assumption: No

[0020] (d) Antisense: No

[0021] (e) Original source: turbot

[0022] Structural features: 2 to 4 highly conserved disulfide bonds formed by 4 to 8 cysteine ​​residues.

[0023] The sequence of SmHep1P comes from hepcidin Hep-1, which has been published (document 1), and its sequence is published online as AY994074.

[0024] Literature 1, ChenSL, LiW, MengL, ShaZX, WangZJ, RenGC. Molecular cloning and expression analysis of hepcidin antimicrobial peptide gene from turbot (Scophthalmus maximus). FishShellfishImmun...

Embodiment 2

[0040] Application of Hepcidin Peptide SmHep1P

[0041] Step 1) Synthesis of Hepcidin Peptide SmHep1P

[0042] The hepcidin peptide in Example 1 above was synthesized by "Shanghai Sangon Bioengineering Technology Service Co., Ltd." with a purity of >90%.

[0043] Step 2) Injection of hepcidin peptide

[0044] Dilute the hepcidin peptide synthesized in the above step 1) to 150ug / ml in PBS, which is the hepcidin peptide dilution. 20 pieces (about 12.7g in weight) were randomly divided into 4 groups, 5 pieces in each group. These 4 groups are named A, B, C and D respectively. Each fish in groups A and C was injected with 100 ul of hepcidin peptide dilution, and each fish in groups B and D (control group) was injected with 100 ul of PBS.

[0045] The composition of PBS is calculated by weight percentage: 0.8%NaCl, 0.02%KCl, 0.358%Na 2 HPO 4 .12H 2 O,0.024%NaH 2 PO 4 , and the balance is water.

[0046] Step 3) Bacteria and Virus Suspension Preparation

[0047] Cultivati...

Embodiment 3

[0052] Application of Hepcidin Peptide SmHep2P

[0053] Step 1) Synthesis of Hepcidin Peptide SmHep2P

[0054] Same as step 1) of Example 2 above.

[0055] Step 2) Injection of hepcidin peptide

[0056] Dilute the hepcidin peptide synthesized in the above step 1) to 150ug / ml in PBS, which is the hepcidin peptide dilution. 20 pieces (about 12.7g in weight) were randomly divided into 4 groups, 5 pieces in each group. These 4 groups are named A, B, C and D respectively. Each fish in groups A and C was injected with 100 ul of hepcidin peptide dilution, and each fish in groups B and D (control group) was injected with 100 ul of PBS.

[0057] Step 3) Bacteria and Virus Suspension Preparation

[0058]Cultivation of Edwardsiella tarda TX1 to OD in LB medium 600 0.8, then centrifuge (5000g, 4°C, 10min), collect the cells, suspend them in PBS to a final concentration of 2x10 6 cfu / ml is the suspension of Edwardsiella tarda. Dilute cytomegalocytivirus RBIV-C1 (see ZhangM, XiaoZ, ...

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Abstract

The invention relates to the field of molecular biology and in particular relates to two small peptides based on turbot hepcidin and application of the peptides. The sequence of the peptides based on the turbot hepcidin is shown in an amino acid sequence SEQ ID No.1 or No2 in a sequence table. The turbot hepcidin peptide is used for resisting bacterial infection or virus infection. After the turbot hepcidin peptides disclosed by the invention are injected, the capacity of fish for resisting virus infection and bacterial infection can be obviously improved.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a turbot hepcidin-based small peptide and its application. Background technique [0002] Hepcidin is a small-molecule cationic antibacterial factor with a molecular weight of about 4kDa. Its protein structure is highly conserved among different species, and it has 2-4 disulfide bonds formed by 4-8 cysteine ​​residues. . Hepcidin has multiple functions. It can regulate the homeostasis of iron in the body by interacting with ferroportin. It is closely related to iron metabolism disorders such as inflammatory anemia and hereditary hemochromatosis. Hepcidin has a wide range of effects on inhibiting the growth and reproduction of bacteria, fungi and viruses, and is an important effector molecule of the human body's "natural immunity". In addition to its small relative molecular weight, high thermal stability, good water solubility, broad bactericidal spectrum and resistance to drugs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00A61K38/16A61P31/12A61P31/04
CPCA61K38/00C07K14/461
Inventor 孙黎张健于兰萍
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI