Bioactive peptide AGYDVEKNNSRIKLGLK and preparation method and application thereof

A bioactive peptide and active technology, applied in the field of protein, can solve problems such as the research of peptide fragments without protein, and achieve the effect of improving the ability, good anti-inflammatory activity, and promoting the increase of induction

Active Publication Date: 2021-04-09
ZHEJIANG PANDA DAIRY GRP COMPANY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the related functions of Histone H1.3 protein polypeptide fragments in the prior art

Method used

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  • Bioactive peptide AGYDVEKNNSRIKLGLK and preparation method and application thereof
  • Bioactive peptide AGYDVEKNNSRIKLGLK and preparation method and application thereof
  • Bioactive peptide AGYDVEKNNSRIKLGLK and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The artificial synthesis of embodiment 1 active peptide AGYDVEKNNSRIKLGLK

[0034] 1. Synthesis of Bioactive Peptides

[0035] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0036] After 2.2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then drained. This was repeated four times, and the resin was drained for use.

[0037] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0038] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0...

Embodiment 2

[0076] The immune activity experiment of embodiment 2 bioactive peptides

[0077] 1. The experiment of bioactive peptide AGYDVEKNNSRIKLGLK promoting macrophages to secrete cytokines (ELISA method)

[0078] 1. Experimental reagents and instruments:

[0079] Reagents: Experimental animal balb / c mice (male 6, 8 weeks old), Shanghai Slack Experimental Animal Co., Ltd.; mouse lymphocyte extract, Shanghai Suo Laibao Biotechnology Co., Ltd.; RPMI1640 medium, GIBCO Company; cattle Serum albumin (bovine serum albumin, BSA), Genebase Company; The mouse spleen lymphocyte-derived bioactive peptide AGYDVEKNNSRIKLGLK obtained in Example 1; ELISA cytokine rapid kit (TNF-α, IL-1β and IL-6) , Wuhan Boster Biological Engineering Co., Ltd.

[0080] Instruments and equipment: LRH, 250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL, 22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150 CO2 incubator Heraeus Company; Dragon Wellsca...

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Abstract

The invention relates to the field of proteins, and particularly relates to a bioactive peptide AGYDVEKNNSRIKLGLK and a preparation method and application thereof. The amino acid sequence of the bioactive peptide AGYDVEKNNSRIKLGLK is Ala-Gly-Tyr-Asp-Val-Glu-Lys-Asn-Asn-Ser-Arg-Ile-Lys-Leu-Gly-Leu-Lys. In-vitro immune function regulation experiments verify that the bioactive peptide AGYDVEKNNSRIKLGLK has a good immune regulation function. The bioactive peptide AGYDVEKNNSRIKLGLK can promote macrophage activation and release cell factors, has the capacity of promoting increase of macrophage nitric oxide induction amount under a certain concentration condition, improves the capacity of resisting external pathogen infection of the body, reduces the morbidity of the body, improves the life quality and has very important significance in developing food, health care products and medicine with the immune regulation function.

Description

technical field [0001] The present invention relates to the protein field, in particular to a biologically active peptide AGYDVEKNNSRIKLGLK and its preparation method and application. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3000 different bioactive peptides have been reported in the current most authoritative bioactive peptide database BIOPEP-UMW. [0003] At present, most studies on bioactive peptides focus on food-derived peptides, while there are few studies and reports on non-food-derived peptides. And it has been confirmed by research results that,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47A61K8/64A61K38/17A61Q19/00A61P29/00A61P37/02A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61P29/00A61P37/02A23L33/18A61K38/00A23V2002/00A23V2200/324
Inventor 张少辉李锡安占文静陈承余肖珊珊石毅洪志骏王娟梁世排梁世赞
Owner ZHEJIANG PANDA DAIRY GRP COMPANY
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