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C4 type pyruvate orthophosphate dikinase (PPDK) gene of corn and application thereof in wheat

A phosphopyruvate double kinase and gene technology, applied in the field of molecular biology, can solve the problems of increasing the photosynthetic rate of transgenic plants and achieve the effect of improving photosynthetic efficiency

Inactive Publication Date: 2014-05-21
HENAN ACAD OF AGRI SCI XIAOMAI INST
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Problems solved by technology

Ji Benhua et al. (2004) found that spraying NaHSO 3 and ATP can increase the photosynthetic rate of transgenic plants, the mechanism may be: transgenic C 4 The activation of PPDK in genetic rice to generate PEP by Pyr is an energy-consuming reaction that requires the participation of ATP, and the detailed mechanism needs further in-depth study

Method used

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  • C4 type pyruvate orthophosphate dikinase (PPDK) gene of corn and application thereof in wheat
  • C4 type pyruvate orthophosphate dikinase (PPDK) gene of corn and application thereof in wheat
  • C4 type pyruvate orthophosphate dikinase (PPDK) gene of corn and application thereof in wheat

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Embodiment 1

[0018] Experimental RNA extraction kits and agarose gel DNA recovery kits were purchased from Tianwei Times Company, MLV reverse transcription kits were purchased from Promega Company, LA-Taq DNA polymerase, pMD-19T cloning vector, Escherichia coli (Escherichia coli ) strain DH5α competent cell preparation kits are all products of TaKaRa Company, plant expression vector pCOMBIA3301 is a commercial product, restriction endonuclease is a product of MBI Company, ampicillin is a product of Amersco Company, and other reagents are imported or domestic analytically pure.

[0019] According to the reported maize pyruvate phosphate dikinase (PPDK) gene cDNA sequence (GenBank accession number: BT054438), a pair of specific primers were designed using Primer5.0 software, and two protective bases were added to each 5' end. PK 1 : 5'-GCagatctTCGGCTCCCTCTCCCCCTTGCTCCAT-3', containing BglⅡ restriction site, PK 2 : 5'-GGttataaCACATCCACCAGCAGCAGGCAATCC-3', containing AanⅠ restriction site. P...

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Abstract

The invention discloses a C4 type pyruvate orthophosphate dikinase (PPDK) gene of corn and an application thereof in wheat. The novel PPDK gene provided by the invention can be stably expressed and inherited in the wheat, and the photosynthetic efficiency is obviously improved in comparison with that of an acceptor material, thus an important gene source and a parent material support are provided for improving the photosynthetic efficiency of C3 type crops by utilizing a C4 type high-photosynthetic efficiency gene and breeding a high-photosynthetic efficiency transgenic wheat variety of which the yield level is greatly increased.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a corn C4-type phosphopyruvate dikinase (PPDK) gene and its application in wheat. Background technique [0002] Wheat is the main food crop in my country, and its production capacity and supply and demand are always related to major strategic issues such as my country's national economic development and food security. From a global perspective, the state of world grain production has always been closely related to the political and economic development of each country. Once grain production and reserves decline, international grain prices will rise sharply, and panic will spread. From a domestic point of view, with the growth of my country's population, the rapid development of industrialization and urbanization, the demand for food will continue to increase. At the same time, since the acreage of grain crops in my country cannot recover significantly, the only way to ensure my c...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N9/12C12N15/82A01H5/00
Inventor 许为钢李艳齐学礼王会伟胡琳张磊
Owner HENAN ACAD OF AGRI SCI XIAOMAI INST
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