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Separating and purifying method of angiotensin converting enzyme inhibitor in ramulus mori skin

A technology for converting enzyme inhibitors and angiotensin, which is applied in the direction of cardiovascular system diseases, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., to facilitate waste reuse, easy access, and reduce pollution Effect

Inactive Publication Date: 2014-06-04
JIANGSU UNIV OF SCI & TECH
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  • Claims
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AI Technical Summary

Problems solved by technology

[0003] However, there has been no report about the extraction process of mulberry bark ACE inhibitors so far, and the development of non-toxic and side-effect-lowering substances using mulberry bark as a raw material will provide more therapeutic means for hypertensive patients

Method used

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  • Separating and purifying method of angiotensin converting enzyme inhibitor in ramulus mori skin

Examples

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Embodiment 1

[0020] A method for the separation and purification of ACE inhibitors in mulberry bark, the steps are as follows:

[0021] 1. Raw material processing: take fresh mulberry branches, peel them, wash them, dry them at 50°C, and crush them through a 20-30 mesh sieve for later use.

[0022] 2. Extraction of active ingredients of mulberry bark ACE inhibitor:

[0023] Weigh the pulverized mulberry bark powder, add water, and prepare a mulberry bark suspension with a mass concentration of 6-8%, add cellulase 3200-3800U / g, adjust the pH to 4.5-5.0, and ultrasonically (power 80 -90W), extract for 60-70min, filter, adjust the pH value of the extract in the range of 6.5-7.5, centrifuge at 5000r / min for 15-20min, separate the supernatant, and obtain the mulberry bark extract.

[0024] 3. Resin separation and purification:

[0025] Select a strong base anion resin 201×7 (produced by Shanghai Jingkai Resin Co., Ltd.) as the chromatography resin, then weigh an appropriate amount of resin, a...

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Abstract

The invention discloses a separating and purifying method of an angiotensin converting enzyme inhibitor in ramulus mori skin; the method comprises steps of: 1, peeling off and crushing and processing the ramulus mori skin; 2, weighting crushed and screened ramulus mori skin, adding water and ramulus mori skin fluid suspension, adding cellulase 3200-3800U / g, adjusting pH value to 4.5-5.0, carrying out supersonic wave cell wall breaking extraction, separation and filtering, and isolating supernatant to obtain ramulus mori skin leach liquid; 3, isolating anion resin 201X7 exchange column chromatography and sephadex G-25 from the ramulus mori skin leach liquid, and freeze drying the isolated products so as to obtain the angiotensin converting enzyme inhibitor in single component and high output; the method is simple in technology, and easy in industrialization scale production.

Description

Technical field: [0001] The invention relates to a method for separating active substances, in particular to a method for extracting and separating blood pressure-lowering active substances (ACE) in mulberry bark. Background technique: [0002] Cortex Mori is a traditional Chinese medicinal material, containing a variety of active ingredients, such as flavonoids, polysaccharides and alkaloids. Studies have shown that Mori twig extract has obvious anti-ulcer, antispasmodic, antibacterial, anti-inflammatory, hypoglycemic, hypolipidemic and analgesic effects on the human body. Wu Zhiping (2005) reported in "Science of Sericulture" that the hypoglycemic effect of mulberry branches is better than that of commonly used mulberry bark and mulberry leaves. Xing Dongjie et al. (2010, China Practical Medicine) and Liu Xianming et al. (2011, Sericulture Science) have shown that the extract of mulberry branches can significantly reduce the levels of plasma triglycerides, total cholester...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/605A61P9/12A61K129/00
Inventor 桂仲争贾俊强刘艳伟
Owner JIANGSU UNIV OF SCI & TECH
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