A small flow cytometer liquid circuit system
A technology of flow cytometry and instrument liquid circuit, which is applied in the field of cell detection and analysis, and can solve the problems of difficult cleaning of sample pipelines and incorrect results, and achieve the effects of thorough and effective cleaning, reduced volume and weight, and improved detection accuracy
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Embodiment 1
[0030] refer to figure 1 As shown, a small flow cytometer liquid circuit system allows the sample to pass through the laser detection area 3 at a certain speed and distribution width under the sheath fluid package, including the sample pipeline 4, the sheath fluid pipeline 15, the waste liquid tube Road 20 and gas pipeline 17;
[0031] A sample injection needle 2, a sample pump 6, and a sample suction needle 7 are sequentially installed on the sample pipeline 4;
[0032] One end of the sheath fluid pipeline 15 is connected to the flow chamber 1 and passes through the flow chamber 1, the other end of the sheath fluid pipeline 15 is connected to the sheath fluid pool 9, and a bladder type A filter element 13 and a sheath liquid pump 12, a first liquid level sensor 1001 is installed at the bottom of the sheath liquid pool 9, and a first filter screen 1101 is installed at the outlet of the sheath liquid pool 9 communicating with the atmosphere;
[0033] The waste liquid pipeline...
Embodiment 2
[0044] Such as figure 2As shown, the sample pipeline 4 is connected in series with the sample pump 6 and the sample pulsation buffer 5, and the sheath fluid pipeline 15 is connected in series with the capsule filter element 13, the sheath fluid pump 12 and the sheath fluid pulsation buffer 14 A pressure sensor 19 is installed at the outlet of the flow chamber 1, and a second filter screen 1102 is installed at the outlet of the micro air pump 18 communicating with the atmosphere.
[0045] The sheath fluid pulsation buffer 14 adopts one of air type, air bag type, and diaphragm type pulsation damper, and the sample pulsation buffer 5 adopts a straight-through pipeline with a smaller inner diameter, the inner diameter is less than 250 μm, or several elastic pipelines The clamper clamps the sample pipeline, and the elastic pipeline clamper includes a clamp 30 and an elastic arm 31, such as Figure 6 shown.
[0046] Since the sample flow rate is 10 μL / min-60 μL / min, the existing ...
Embodiment 3
[0050] Such as image 3 As shown, the sheath liquid pump 12 is placed in the waste liquid pipeline 20, and acts as a waste liquid pump to drive the waste liquid mixed with the sample and the sheath liquid into the waste liquid pool. The flow of the sheath fluid is driven by the pressure difference between the sheath fluid pump 12 and the sample pump 6 .
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