A method for quantitative detection of tobacco lignin by mid-infrared spectroscopy

Abstract

The invention discloses a method for quantitative detection of tobacco lignin by adopting mid-infrared spectroscopy. The method comprises the steps of finding out a characteristic absorption peak from the measured infrared spectrogram, and measuring the area of the peak; importing the original data of the measured infrared spectrogram into PeakFi tv 4.12 software, and carrying out fitting peak separation on the 1508 cm<-1> characteristic peak. According to the method, the fitting correlation coefficient of the standard curve of a lignin reference object reaches 0.9914, so that the requirement of accurate quantization can be reached, the detection result is accurate, and the repeatability is good. The method overcomes the defect that the existing chemical detection method consumes a great deal of time and reagent, and solves the problem that in the existing quantitative analysis method, the lignin in tobaccos is difficult to separate and purify; an in-situ analysis method of the tobacco lignin is established scientifically; the method can be used for conveniently, rapidly and accurately measuring the content of the lignin of multiple tobacco stem powders.

Description

Technical field [0001] The invention belongs to the technical field of tobacco analysis and detection, and specifically relates to a method for quantitatively detecting tobacco lignin by applying mid-infrared spectroscopy. Background technique [0002] Lignin is the main component of benzene ring in tobacco leaves, so it is also the main source of harmful substances such as polycyclic aromatic hydrocarbons and aromatic amines in tar. Cell wall materials such as lignin and cellulose do not change during tobacco processing and are considered to be stable compounds in tobacco. Therefore, in-depth study of the content, distribution and structure of lignin in tobacco leaves, establishes methods for the separation, purification and structural characterization of tobacco lignin, grasps the reaction mechanism of tobacco lignin under burning conditions, and establishes regulatory pathways to reduce the generation of harmful substances , Is of far-reaching significance to the tobacco indu...

AI Technical Summary

Problems solved by technology

[0004] At present, the determination of tobacco lignin basically adopts traditional chemical methods, which require a lot of time and reagents

At the same time, tobacco leaves are rich in protein, starch, pectin, oil and other organic matter, but the lignin content is low, which causes great difficulties in the separation and purification of lignin.

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