Microscopic section rapid digital scanning device and method with real-time focusing function

A technology of microsectioning and digital scanning, applied in the field of microscopy, which can solve the problems of loss of focal plane, large measurement range, poor accuracy, etc.

Active Publication Date: 2014-06-11
MOTIC CHINA GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The common disadvantage of this type of method is: first, in addition to the camera required for imaging, a special camera for focusing is also required, so the focusing device caused by this type of method is expensive
Second, the focusing speed caused by this method will be low, because the imaging process and image transmission speed are low due to the large amount of data. The frame rate of most cameras is only 15fps. Even if the calculation and comparison time is ignored, a frame rate of 15fps The camera can achieve the fastest focal plane measurement cycle in 60 ms
The third shortcoming is that the defocus distance measurement range and accuracy cannot be taken into account: the camera with a high inclination has a large measurement range but poor accuracy. Conversely, a small camera inclination has high measurement accuracy but a small measurement range, which may easily cause focal plane loss.
The fourth disadvantage is that the real-time focusing device is bulky and has high requirements for placement
However, the

Method used

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  • Microscopic section rapid digital scanning device and method with real-time focusing function
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  • Microscopic section rapid digital scanning device and method with real-time focusing function

Examples

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Example Embodiment

[0063] Example one:

[0064] Combined with Figure 1 (A), Figure 1 (B), Figure 2 (A), 2 (B).

[0065] The present invention is a microslice fast digital scanning device with real-time focusing, which is a scanning device for quickly acquiring all or a considerable part of the digital images of microslices, including: a digital microscopy imaging system 1 and a Real-time focusing device 2 matched with it. The digital microscopic imaging system 1 includes at least a computer 3, an image detection device 4 connected to the computer for photoelectric conversion, and a microscopic optical imaging system 5 between the sample 7 placed on the automatic stage 6 and the image detection device 4 . The microscopic optical imaging system 5 includes at least one objective lens 11, a tube lens 18 matched with it, and a filter cube may be placed between the objective lens and the tube lens. The digital microscopic imaging system 1 at least further includes an illumination optical system 8 and an...

Example Embodiment

[0077] Embodiment two:

[0078] Combined with Figure 1 (A), Figure 1 (B), Figure 3 (A), 3 (B).

[0079] Compared with the first embodiment, this embodiment only expands the detection range of the defocus amount of the first embodiment. Therefore, except for placing a few more optical signal detection elements, the rest of the components are the same, and the description will not be repeated here. The following describes only the special part of this embodiment, that is, the defocus measurement part.

[0080] When the defocus distance 2d that can be effectively measured by the pair of optical signal detection elements along the first straight line direction cannot meet the requirements, multiple pairs of optical signal detection elements can be placed along the straight line. Figure 3 (A) shows the situation where two pairs of 4 optical signal detection elements are placed, and they are projected along the X direction on the XY plane. The focal plane defocus range that can be measure...

Example Embodiment

[0086] Embodiment three:

[0087] This embodiment is compared with the first embodiment in that a pair of optical signal detection elements are placed in the first linear direction, they are projected on the XY plane along the X direction, and they form an angle α with the XY plane. On this basis, a second pair of optical signal detection elements are placed in the second linear direction, the angle between them and the XY plane is also α, and the projection of the second pair on the XY plane is parallel to the Y direction. Therefore, compared with the first embodiment, except for placing a pair of optical signal detection elements in the Y direction, the rest of the components are the same, and the description will not be repeated here.

[0088] In this embodiment, the defocus measurement range of the photoelectric conversion projected in the X / Y direction on the XY plane is between [-d, d]. When the defocus measurement device along the X direction determines the defocus amount a...

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Abstract

The invention discloses a microscopic section rapid digital scanning device and method with the real-time focusing function. The microscopic section rapid digital scanning device comprises a digital microscopic imaging system and a real-time focusing device combined with the digital microscopic imaging system in a matched mode. The digital microscopic imaging system comprises a computer, an image detecting device for achieving photovoltaic conversion, a microscopic optical imaging system, an automatic carrying table, a movement and control system corresponding to the automatic carrying table, a microscope lighting optical system, a lighting light source and the like. The real-time focusing device comprises a device for measuring the defocusing amount of a sample and a device for driving the sample or a lens to move and enabling the sample and the focal plane of the lens to be coincided. According to the microscopic section rapid digital scanning device and method, the distance between the sample and the focal plane of the imaging system in the section scanning process can be detected in real time, real-time focusing can be achieved, and therefore an excellent image is obtained; the time-consumed focal plane modeling process can be omitted through the real-time focusing technology; meanwhile, the large focusing range and the high focusing accuracy are both considered; in addition, the microscopic section rapid digital scanning method is high in efficiency and low in cost.

Description

technical field [0001] The invention belongs to the technical field of microscopes, in particular to a fast digital scanning device for microsections with real-time focusing and a method thereof. Background technique [0002] In teaching, remote pathological diagnosis, software-based intelligent automatic identification system and other applications, it is necessary to provide the whole picture of the tissue sample observed under the microscope for multiple people to read, or to read in different places, or at different times when there is neither actual section nor microscope Next read, diagnose, or permanently save. These applications all require the digitization of the entire or substantial portion of a tissue sample. To meet this demand, the full-slice digital rapid scanning system for microsections, also known as the virtual microscopic imaging system, is a highly integrated device that integrates microscopic optical imaging technology, digital imaging technology, and ...

Claims

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Application Information

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IPC IPC(8): G02B21/24
Inventor 易定容
Owner MOTIC CHINA GRP CO LTD
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