Seminal plasma composite quality control product and its preparation method and kit

A quality control product and kit technology, which is applied in the preparation of test samples, biological testing, material inspection products, etc., can solve the problems of increasing the technical difficulty of seminal plasma quality control products, difficult to store, and unstable, and achieves a high level of avoidance. Unstable, avoids hard-to-save, stable performance effects

Active Publication Date: 2016-09-14
BRED LIFE SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many substances in semen are very unstable in vitro and difficult to preserve, which increases the technical difficulty of preparing seminal plasma quality control products
[0003] The quality control materials used in the detection kits for various items of seminal plasma in the prior art are only proprietary quality control materials for their respective detection items, and are not prepared from seminal plasma. The finished quality control products are applied to the detection kits of various items in the seminal plasma

Method used

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  • Seminal plasma composite quality control product and its preparation method and kit
  • Seminal plasma composite quality control product and its preparation method and kit
  • Seminal plasma composite quality control product and its preparation method and kit

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preparation example Construction

[0023] The preparation method of above-mentioned seminal plasma composite quality control product is:

[0024] Preparation of quality control preservation solution: Weigh the above-mentioned sugars, salts and enzyme stabilizers of a certain weight, mix them, add purified water to 100ml, fully dissolve, and obtain a quality control preservation solution containing the above-mentioned percentages of each component.

[0025] Obtain seminal plasma stock solution: take several parts of fresh human semen that have been completely liquefied, select HCV, HIV1+2, HBSAg and syphilis negative as raw materials, and centrifuge to obtain seminal plasma (you can also use frozen semen or semen Plasma stock solution is used as raw material, and HCV, HIV1+2, HBSAg and syphilis also need to be detected), and the collected seminal plasma stock solution is mixed by physical methods to obtain seminal plasma stock solution.

[0026] Mix and freeze-dry to obtain seminal plasma composite quality contr...

Embodiment 1

[0037] This embodiment provides a seminal plasma composite quality control product, including: a quality control product preservation solution and a seminal plasma stock solution; the quality control product preservation solution is an aqueous solution containing glycine, sodium chloride and sucrose; wherein, the The quality control product preservation solution and the seminal plasma stock solution were mixed at a volume ratio of 1:1, and then freeze-dried to form a dry powder.

[0038] This embodiment also provides a method for preparing the above-mentioned seminal plasma composite quality control product, the steps of which are as follows:

[0039] Step 1: Prepare quality control preservation solution

[0040] Weigh 0.7302g of aminoacetic acid, 0.1205g of sodium chloride and 0.5713g of sucrose, then add purified water to 100ml, fully dissolve, and obtain a quality control preservation solution.

[0041] Step 2: Obtain seminal plasma stock solution

[0042] Take several pa...

Embodiment 2

[0046] The difference between this embodiment and embodiment 1 is:

[0047] The quality control preservation solution is an aqueous solution containing polyvinylamine, sodium carbonate and glucose; wherein, the quality control preservation solution and the seminal plasma stock solution are mixed at a volume ratio of 1:80 and then freeze-dried to form a dry powder.

[0048] The preparation method of the quality control preservation solution is as follows: weigh 0.8134g of polyvinylamine, 0.0954g of sodium carbonate and 0.6543g of glucose, then add purified water to 100ml, fully dissolve, and obtain the quality control preservation solution.

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Abstract

The invention provides a seminal plasma composite quality control product, which is a freeze-dried powder made by mixing a quality control product preservation solution and a seminal plasma original solution; the quality control product preservation solution contains 0.47% to 0.67% sugar and salt. An aqueous solution of 0.07 to 0.17% of semen and 0.63 to 0.83% of enzyme stabilizer; the seminal plasma solution is the seminal plasma obtained by direct centrifugation of completely liquefied fresh human semen; the quality control product preservation solution is mixed with the seminal plasma solution The volume ratio is 1:100~100:1. The present invention uses a unique quality control product preservation solution, mixes it with the seminal plasma at an appropriate volume ratio and then freeze-dries it. This can make various biochemical indicators in the seminal plasma more stable, thereby effectively avoiding the in vitro storage of substances in the semen. It has the disadvantages of being very unstable and difficult to preserve. The prepared seminal plasma composite quality control product has stable performance and is easy to use, and can be used for quality control of seminal plasma biochemical testing projects.

Description

technical field [0001] The invention belongs to the category of in vitro detection reagents, in particular to a seminal plasma composite quality control product, a preparation method and a kit thereof. Background technique [0002] There has been a lack of experimental quality control materials for the detection of biochemical indicators in semen, which makes the standardization of semen biochemical analysis stagnant. Many substances in semen are very unstable in vitro and difficult to preserve, which increases the technical difficulty of preparing seminal plasma quality control products. [0003] The quality control materials used in the detection kits for various items of seminal plasma in the prior art are only proprietary quality control materials for their respective detection items, and are not prepared from seminal plasma. The finished quality control products were used in the detection kits for various items of seminal plasma. Contents of the invention [0004] T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N33/48G01N33/50
CPCG01N33/96A01N1/021C12N9/96
Inventor 程浩胡家纯陶思恩杨红芳邓少君
Owner BRED LIFE SCI TECH
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