Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for detecting the content of water-soluble components in sarsaparilla

A detection method and water-soluble technology, which is applied in the content detection of water-soluble components, and in the field of content detection of water-soluble components of Chinese herbal medicine Smilax or plants of the same genus, can solve the problems of difficult distinction and mixed use of different species, achieve good stability, good reproducibility

Active Publication Date: 2016-03-09
GUILIN SANJIN PHARMACEUTICALS CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a commonly used bulk medicinal material, Smilax is widely used clinically, and it is difficult to distinguish between different species of this genus, and there is a phenomenon of mixed use of different species. Therefore, it is urgent to find an operable detection method to establish a more specific, stable, Controllable quality standards to ensure safe and controllable clinical medication of Smilax

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for detecting the content of water-soluble components in sarsaparilla
  • A method for detecting the content of water-soluble components in sarsaparilla
  • A method for detecting the content of water-soluble components in sarsaparilla

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] [Example 1] Content detection method of sarsaparilla water-soluble components

[0047] 1) Chromatographic conditions

[0048] Chromatographic column: YMCpackODS-AQ liquid chromatography column (4.6×250mm5μm); mobile phase: volume ratio 10:8:64 methanol-acetonitrile-0.2% phosphoric acid solution; detection wavelength: chlorogenic acid is 325nm, HeliciosideA is 283nm; column temperature : 35℃; flow rate: 1.0ml min -1 , the corresponding retention times of chlorogenic acid and HeliciosideA are 7.3min and 13min respectively;

[0049] 2) Preparation of the test solution

[0050] Take 1.5g of sarsaparilla powder, weigh it accurately, put it in a stoppered Erlenmeyer flask, accurately add 25ml of 80% methanol as the extraction solvent, seal it tightly, weigh it, extract it by ultrasonic for 45min (power 250W, frequency 40kHz), let it cool, Weigh the weight, use the extraction solvent to make up for the lost weight, shake well, filter, and take the filtrate to obtain the fin...

Embodiment 2

[0055] [Example 2] The content detection method of the water-soluble components of Sarsaparilla

[0056] The specific operation steps are the same as in Example 1, and the difference from Example 1 is that the extraction solvent in step 2) is 50% methanol.

Embodiment 3

[0057] [Example 3] Content detection method of Smilax sarsaparilla water-soluble components

[0058] The specific operation steps are the same as in Example 1, and the difference from Example 1 is that the extraction solvent in step 2) is ethanol.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of quality detection of traditional Chinese herbals, and specifically relates to a method for measuring the content of water soluble components in bullbrier. The method is mainly used to measure the contents of chlorogenic acid and Helicioside A in medicinal bullbrier, the content of chlorogenic acid must be more than or equal to 0.10%, and the content of Helicioside A must be more than or equal to 0.05%. Through optimizing the chromatogram conditions and adoption of a wavelength conversion HPLC method, the method can simultaneously measure the water soluble medicinal components namely chlorogenic acid and Helicioside A for the first time, and thus has a very important meaning for detection of bullbrier, detection of Chinese patent medicine made of bullbrier, and establishment of detection standards of bullbrier and Chinese patent medicine made of bullbrier. The detection method provided by the invention can comprehensively detect the water soluble representative active components in bullbrier, and has the characteristics of rapidness, good repeatability, and good stability.

Description

technical field [0001] The invention belongs to the field of quality control of Chinese medicinal materials, in particular to a method for detecting the content of water-soluble components of the Chinese medicinal material Smilax smilax or plants of the same genus, and also includes a method for detecting the content of water-soluble components of Chinese medicinal preparations containing smilax as raw materials. Background technique [0002] Smilax is Liliaceae plant Smilaxchina (SmilaxchinaL.), long-supporting Smilax (S.feroxWall.exKunth), black-fruited Smilax (S.glauco-chinaWarb.), holding handle Smilax (S.discotisWarb.), small Dried rhizomes of S. davidiana A.DC, S. biumbellata Warb., S. chingii Wanget Tang, S. polycolea Warb. and other plants. Excavated from late autumn to next spring, removed fibrous roots, washed, dried in the sun or sliced ​​while fresh, and dried. Sweet in taste, slightly bitter, flat in nature, returns to Liver and Kidney Meridians. Traditional m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 邹节明周艳林
Owner GUILIN SANJIN PHARMACEUTICALS CO LTD