Separation and purification of Auricularia phycocyanin by magnetic nanoparticles
A magnetic nanoparticle, separation and purification technology, applied in the separation and purification of phycocyanin, the separation and purification of phycocyanin, can solve the problems of expensive chromatographic packing material, long time-consuming, cumbersome steps, etc., and achieve simple method and high recovery rate , Strengthen the effect of separation speed
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Embodiment 1
[0028] 5.5g FeCl 3 .6H 2 O and 2.8 g FeSO 4 .7H 2O was dissolved in 50 mL of deionized water, at room temperature, under the protection of nitrogen, 2 mol / L sodium hydroxide solution was slowly added dropwise therein until the pH value of the solution was 11, and the reaction was stopped after stirring for 60 min. After using magnetic separation, use alcohol-water mixed solution and deionized water several times until the particle surface becomes neutral to obtain magnetic Fe 3 o 4 Nanoparticles. 0.5g magnetic Fe 3 o 4 Put the nanoparticles into a mixed solution of 150mL ethanol and water (volume ratio 4:1), adjust the pH value to 10 with 2mol / L sodium hydroxide solution, and add 30mL ethyl silicate alcohol dropwise under nitrogen protection. Water mixed solution (containing 1.0 mL of ethyl silicate) was reacted at room temperature for 5 h. The solid particles obtained by magnetic separation are washed three times with alcohol-water mixed solution, ionized water, and a...
Embodiment 2
[0032] 5.5g FeCl 3 .6H 2 O and 2.8 g FeSO 4 .7H 2 O was dissolved in 50 mL of deionized water, and at room temperature, under the protection of nitrogen, 2 mol / L sodium hydroxide solution was slowly added dropwise therein until the pH value of the solution was 11, and the reaction was stopped after stirring for 4 h. After using magnetic separation, use alcohol-water mixed solution and deionized water several times until the particle surface becomes neutral to obtain magnetic Fe 3 o 4 Nanoparticles. 0.5g magnetic Fe 3 o 4 Put the nanoparticles into a mixed solution of 150mL ethanol and water (volume ratio 4:1), adjust the pH value to 10 with 2mol / L sodium hydroxide solution, and add 30mL ethyl silicate alcohol dropwise under nitrogen protection. Water mixed solution (containing 1.0 mL of ethyl silicate) was reacted at room temperature for 5 h. The solid particles obtained by magnetic separation are washed three times with alcohol-water mixed solution, ionized water, and...
Embodiment 3
[0036] The magnetic nanoparticles in Example 2 were washed with 0.1 mol / L hydrochloric acid, then washed with deionized water to be neutral, and dried for future use.
[0037] Take 100g of ground fungus powder, add 1.5L of 0.02mol / L phosphate buffer solution (pH=7.2), and soak for 4 hours at 25°C. After soaking, put it into a 1000W, 40kHz ultrasonic breaker, turn on the ultrasonic wave for 60min, and break the wall of the ground fungus powder cells. The solid powder was removed by filtration, and the supernatant was taken for later use. 10 g of magnetic nanoparticles were added thereto, mixed and placed in a constant temperature oscillator, adsorbed for 48 hours at 20° C. at a speed of 150 r / min, and magnetically separated to obtain solid particles therein. The saturated magnetic nanoparticles were added to 500 mL of phosphate buffer (pH=4.5), placed in a constant temperature oscillator at 37° C., and desorbed at a speed of 150 r / min for 24 hours. The desorption solution was...
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