Separation method of alfalfa extract active components in alfalfa

A technology of alfalfa and active ingredients, applied in the biological field, can solve the problems of resource consumption, inability to adjust the content of active ingredients according to the situation, and unsatisfactory refined production, and achieve the effect of reducing energy consumption and solvent consumption

Inactive Publication Date: 2014-07-09
SICHUAN BAOZIMU TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the separation process of alfalfa, the active substances are extracted separately, which consumes a lot of resources
Only alfalfa is extracted, but the active components are not separated, and the content of active components cannot be adjusted according to the situation during use, which does not meet the requirements of refined production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] After the alfalfa is dried and pulverized, it is soaked in ether first, and then the ether is separated and removed. Add distilled water to the ether-soaked product, boil and reflux for extraction twice for 1 hour, combine the extracts and concentrate 1 / 2 volume, and separate the residue. The concentrate was poured into methanol, precipitated, and the precipitate was separated. The precipitate was washed twice with ether to obtain alfalfa polysaccharide, one of the active substances of alfalfa. The separated filtrate was concentrated until it had no alcohol smell. Add distilled water to the filtrate concentrate, extract 3 times with n-butanol, concentrate 1 / 2 volume after extraction, pour into ethyl acetate, precipitate, separate the precipitate, wash the precipitate twice with ether to obtain the alfalfalin active substance One alfalfa saponins. Add 60%~70% methanol to the residue boiled and refluxed in distilled water, reflux and extract twice for 1.5 hours, and the ...

Embodiment 2

[0020] After the alfalfa is dried and pulverized, it is soaked in ether first, and then the ether is separated and removed. Add distilled water to the ether-soaked product, boil and reflux for extraction three times for 2 hours, combine the extracts and concentrate 1 / 2 volume, and separate the residue. The concentrate was poured into methanol, precipitated, and the precipitate was separated. The precipitate was washed twice with ether to obtain alfalfa polysaccharide, one of the active substances of alfalfa. The separated filtrate was concentrated until it had no alcohol smell. Add distilled water to the filtrate concentrate, extract 3 times with n-butanol, concentrate 1 / 2 volume after extraction, pour into ethyl acetate, precipitate, separate the precipitate, wash the precipitate twice with ether to obtain the alfalfalin active substance One alfalfa saponins. Add 60%~70% methanol to the residue boiled and refluxed in distilled water, reflux and extract 4 times, the extractio...

Embodiment 3

[0022] After the alfalfa is dried and pulverized, it is soaked in ether first, and then the ether is separated and removed. Add distilled water to the ether-soaked product, boil and reflux for extraction twice for 1 hour, combine the extracts and concentrate 1 / 2 volume, and separate the residue. The concentrate was poured into methanol, precipitated, and the precipitate was separated. The precipitate was washed twice with ether to obtain alfalfa polysaccharide, one of the active substances of alfalfa. The separated filtrate was concentrated until it had no alcohol smell. Add distilled water to the filtrate concentrate, extract 3 times with amyl alcohol, concentrate 1 / 2 volume after extraction, pour into ethyl acetate, precipitate, separate the precipitate, wash the precipitate twice with ether, and obtain one of the active substances of alfalfa Alfalfa saponins. Add 60%~70% methanol to the residue boiled and refluxed in distilled water, reflux and extract for 3 times, the ext...

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PUM

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Abstract

The invention discloses a separation method of alfalfa extract active components in alfalfa, which comprises the following steps: (a) drying alfalfa, soaking with ether; (b) adding distilled water into the product of step (a), performing boiling reflux extraction, combining the extract I, separating residues; (c) adding 60%-70% methanol into the residues of step (b), performing reflux extraction; (d) allowing the methanol in step (b) to precipitate, separating to obtain a precipitate of alfalfa polysaccharide, concentrating the separated filtrate to have no alcohol smell; (e) extracting the filtrate concentrate of step (d) with n-butyl alcohol, precipitating with ethyl acetate, separating the precipitate, washing the precipitate with ether twice to obtain alfalfa saponins; (f) eluting the concentrate in step (c) by a S-8 macroporous resin column with methanol as a solvent and with a volume ratio of methanol to water of 4:1, so as to obtain alfalfa flavones. The invention provides a separation method of alfalfa extract active components in alfalfa, which separates alfalfa extract active components by the same process, thus saves solvents and energy consumption, and facilitates adjustment of active component contents.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for separating active components of alfalfa from alfalfa. Background technique [0002] Alfalfa is an alfalfa extract with flavonoids, saponins and alfalfa polysaccharides as its main active ingredients. Alfalfa polysaccharide has pharmacological effects such as immune regulation, anti-tumor, anti-virus, anti-radiation, anti-aging, anti-infection, etc. It can also improve animal production capacity, and has little toxic and side effects on the substrate. Alfalfa flavonoids, within a certain measurement range, can obviously promote the growth of poultry and livestock, improve fecundity and enhance the body's immunity, etc. It has anti-tumor, anti-aging, and enhanced cardiovascular functions on the human body, and it can also treat chronic prostate cancer. Inflammation, enhance immunity, regulate endocrine system, protect liver, anti-allergic, antibacterial, anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07J63/00C07D311/30C07D311/40
Inventor 姚书兰
Owner SICHUAN BAOZIMU TECH
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