Molecular markers related to meat quality traits and their applications
A meat quality, molecular marker technology, applied in the determination/inspection of microorganisms, DNA preparation, DNA/RNA fragments, etc., to achieve significant application value and social benefits, speed up the breeding process, and label reliable results
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Embodiment 1
[0026] Discovery and typing of embodiment 1 SNP
[0027] DNA pool was used to screen SNPs, 10 samples of Xuelong black cattle were randomly selected for DNA pool pooling, and frozen semen genomic DNA was extracted, and its concentration was adjusted to about 100ng / μl, and its concentration was detected by ultraviolet spectrophotometer and reached uniformity. A total of 100 μl of DNA was mixed with 10 μl from each individual to construct a DNA pool.
[0028] A sequence of DNMT3a intron 11 in the mixed pool DNA was amplified by PCR.
[0029] The primer pairs for PCR amplification are as follows:
[0030] Forward primer F: 5'-agcactggattgaggtcctg-3';
[0031] Reverse primer R: 5'-gccaagtctgtcctttgagg-3'.
[0032] The target sequence for the above primer pair is as follows: SEQ ID No.1
[0033] The PCR reaction system is shown in Table 1.
[0034] Table 1 PCR reaction system
[0035] Reagent
Dosage (μl)
wxya 2 0
17.375
l0×Buffer amplification ...
Embodiment 2
[0039] Example 2 Association analysis of SNP sites and meat quality traits
[0040] The experimental samples in this example are a group of 366 individual beef cattle from 13 different breeds at home and abroad, including 30 Sanhe cattle, 30 Xinjiang brown cattle, 3 Bohai black cattle, 30 Luxi cattle, and Fuzhou cattle. 7 state cattle, 24 Limousin cattle, 11 Wagyu cattle, 29 Charolais cattle, 9 Angus cattle, 4 Norwegian Red cattle, 14 Montbelia cattle, 28 Simmental cattle and Xuelong 141 black cattle. The selected samples were mostly frozen semen samples, 6 of Wagyu cattle were ear tissue samples, and 141 Xuelong black cattle were all muscle samples.
[0041] 1. Genotyping
[0042] 1. Genomic DNA extraction
[0043]The traditional phenol imitation method was used to extract genomic DNA from frozen semen samples, and the genomic DNA was extracted from tissue samples using kit DP304 (Tiangen Biotech Co., Ltd, Beijing, China). Genomic DNA was subjected to 1% agarose gel elect...
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