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Method for domesticating dendrobium officinale kimuraet migo tissue culture seedling by using glass bottle

A technology of dendrobium candidum and tissue culture seedlings, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low survival rate of tissue culture seedlings transplanted, difficulty in obtaining bat feces, and increased cultivation costs, etc., to achieve Overcome the effect of easy festering and death, maintain continuity, and thicken leaves

Inactive Publication Date: 2014-07-16
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the low survival rate of transplanted tissue culture seedlings is common at present, and the intermediate link from bottle seedlings to field cultivation has become a major obstacle restricting the production and development of Dendrobium officinale
For example, the Chinese patent "Planting Method of Dendrobium officinale" (applicant: Baishidong Tianxianshan Dendrobium Professional Cooperative in Guiping City; application date: November 30, 2013; application number: CN201310623199.9) discloses a planting method of Dendrobium officinale In the method, raw materials such as peat soil, peanut shells, pine chaff, pine bark, and a small amount of bat droppings are used to powder as substrates to cultivate Dendrobium officinale, but the bat droppings described in this method are difficult to obtain, and the substrate needs to be prepared at 100 Use after treatment at ~110°C, which increases the cost of cultivation to a certain extent, is relatively cumbersome, and there are also cases where the survival rate of tissue culture seedlings is low when transplanted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for domestication of dendrobium candidum tissue culture seedlings in glass bottles, said method comprising the following steps:

[0019] 1) Select mature culture bottle seedlings;

[0020] 2) Shade pre-cultivation of bottle seedlings;

[0021] 3) Remove the agar and sterilize the bottle seedlings;

[0022] 4) The bottle seedlings are dried and dehydrated;

[0023] 5) The seedlings in the bottle were put into the bottle again and the lid was half opened for cultivation, and finally transplanted into the seedbed matrix to obtain the tissue cultured seedlings of Dendrobium officinale.

[0024] Further, the mature cultured bottle seedlings in step 1) refer to seedlings with a height of 5-8 cm, obvious stem nodes, and 4-6 roots.

[0025] Further, in step 2), the shaded pre-cultivation of bottle seedlings refers to cultivating in plastic greenhouses or greenhouses that can shelter from the rain, with a shading degree of about 70%, pre-cultivating for 20-30 days, a...

Embodiment 2

[0034] This example is an optimization scheme based on Example 1, and provides a method for domesticating Dendrobium officinale seedlings in glass bottles. For the parts in this embodiment that are the same as those in Embodiment 1, please refer to the content disclosed in Embodiment 1 for understanding, and the content disclosed in Embodiment 1 should also be regarded as the content of this embodiment, and will not be described repeatedly here.

[0035] The method comprises the steps of:

[0036] 1. Select mature tissue culture seedlings of Dendrobium officinale: Select seedlings with a seedling height of 5-8 cm, obvious stem nodes, and tissue-cultured seedlings with 4-6 roots as bottle seedlings.

[0037] 2. Shading pre-cultivation of bottle seedlings: The bottle seedlings selected in step 1) were cultivated in a rain-proof plastic greenhouse with a shading degree of about 70%, pre-cultured for 20-30 days, and the bottle cap was opened for pre-cultivation 3 days before t...

Embodiment 3

[0044] This example is an optimization scheme based on Example 1, and provides a method for domesticating Dendrobium officinale seedlings in glass bottles. For the parts in this embodiment that are the same as those in Embodiment 1, please refer to the content disclosed in Embodiment 1 for understanding, and the content disclosed in Embodiment 1 should also be regarded as the content of this embodiment, and will not be described repeatedly here.

[0045] The method comprises the steps of:

[0046] 1. Select mature tissue culture seedlings of Dendrobium officinale: Select seedlings with a seedling height of 5-8 cm, obvious stem nodes, and tissue-cultured seedlings with 4-6 roots as bottle seedlings.

[0047] 2. Shading pre-cultivation of bottle seedlings: The bottle seedlings selected in step 1) were cultivated in a rain-proof plastic greenhouse with a shading degree of about 70%, pre-cultured for 20-30 days, and the bottle cap was opened for pre-cultivation 3 days before t...

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PUM

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Abstract

The invention belongs to the technical field of plant tissue culture rapid propagation, and provides a method for a domesticating dendrobium officinale kimuraet migo tissue culture seedling by using a culture bottle. The method provided by the invention comprises the following steps of selecting a mature tissue culture bottle seed, overshadowing and pre-culturing the bottle seed; removing agar for the bottle seed and disinfecting, drying and dehydrating the bottle seed, placing the bottle seed in a bottle to cultivate by opening a cover half, and finally transplanting into a seedbed matrix. The method provided by the invention has the advantages that the operation is simple, the cost is low, the technical problem that the tissue culture seedling of the dendrobium officinale kimuraet migo is easy to fester and die after leaving the bottle, and the planting surviving rate is low are effectively solved, after the tissue culture seedling leaves the bottle in a short time, the laminae of the tissue culture seedling fundamentally form a thorough horny layer, the effective photosynthesis is can be carried out well, and different substances are synthesized; a root of the tissue culture seedling domesticated by the method in the invention is developed, the laminae are thick, the color of the laminae is dark green, the stem is thick and hard, the growing consistency is kept, and the surviving rate after transplanting can achieve more than 95%.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and rapid propagation of plants, and in particular relates to a method for domesticating dendrobium officinale Kimura et Migo tissue culture seedlings through culture bottles. Background technique [0002] Dendrobium officinale Kimura et Migo is a precious traditional Chinese medicine commonly used in my country. It is a perennial herb of the Orchidaceae Dendrobium genus. Various symptoms such as irritability and post-illness deficiency heat. Wide range of uses and high demand. Due to long-term uncontrolled excavation and low regeneration capacity of its own resources, the natural resources of Dendrobium officinale have been gradually exhausted. At present, this plant has become one of the national key protected medicinal materials. Artificial planting of Dendrobium officinale has broad prospects, and the scale of planting is constantly expanding. Tissue culture, rapid propagation and lar...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李娟林位夫张朝阳
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI