Chiral resolution of prazole compound by aqueous two-phase system
A two-phase system, technology of azole compounds, applied in the field of medicine and chemical industry, can solve the problems such as chiral separation that has not been deeply researched and applied, no application of chiral separation of prazole compounds, environmental pressure, etc.
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[0013] The following specific embodiments are intended to explain the content of the present invention.
[0014] 1. Add 1.0 g of omeprazole and 2.0 g of β-cyclodextrin into 10 ml of ammonia buffer solution with pH 11.0. Add 20ml of 40% ethanol solution containing 2g of diisopropyl tartrate, and stir evenly. Slowly and uniformly add 3.0 g of ammonium sulfate, stir evenly, and stir at high speed for 30 minutes. After standing for 30 minutes, the layers were separated and the two phases were separated. Adjust the pH of the ethanol phase to 7.0 with glacial acetic acid, crystallize, centrifuge, and dry at low temperature to obtain the R-omeprazole chiral compound with an ee value of 99.3%. The ethanol phase can be concentrated and extracted with an equal amount of toluene, and crystallized at low temperature. Centrifuge to recover part of the R-omeprazole chiral compound. Adjust the pH of the water phase to 7.0 with glacial acetic acid, crystallize, centrifuge, and dry at 45°C ...
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