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A method for preparing stable functional casein hydrolyzate by using different hydrolysis conditions

A kind of caseinase and functional technology, applied in the field of casein hydrolysis technology processing, can solve the problem of not being able to hydrolyze the same conditions, and achieve the effect of ensuring stability

Active Publication Date: 2016-08-31
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in industrial production, it is difficult to completely control these five hydrolysis conditions, and due to the limitation of production conditions, different production batches often cannot guarantee that the hydrolysis conditions are the same every time

Method used

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  • A method for preparing stable functional casein hydrolyzate by using different hydrolysis conditions
  • A method for preparing stable functional casein hydrolyzate by using different hydrolysis conditions
  • A method for preparing stable functional casein hydrolyzate by using different hydrolysis conditions

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Experimental program
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Effect test

Embodiment 1

[0039]The mass concentration of casein substrate is 5%, and the hydrolysis temperature is controlled by circulating water bath to 50±0.2°C. According to the ratio of enzyme to substrate protein (E / S) 1 / 100, alkaline protease is added, and by adding 0.1M Standard alkali controls the pH to 8.5±0.1 to reach the corresponding degree of hydrolysis (DH), heats at 95ºC for 10 minutes to inactivate the enzyme, and dries to obtain the enzymatic hydrolyzate product. Under these conditions, hydrolysates with degrees of hydrolysis of 5%, 10% and 15% were prepared. The soluble nitrogen content of DH5%, 10% and 15% TCA was determined to be 54.36%, 64.31% and 75.34%, respectively, and the DPPH clearance rate was 34.77%, 53.62% and 69.78%, respectively.

Embodiment 2

[0041] The mass concentration of casein substrate is 5%, and the hydrolysis temperature is controlled by circulating water bath to 50±0.2°C. According to the ratio of enzyme to substrate protein (E / S) 2 / 100, alkaline protease is added, and by adding 0.1M Standard alkali controls the pH to 8.5±0.1 to reach the corresponding degree of hydrolysis (DH), heats at 95ºC for 10 minutes to inactivate the enzyme, and dries to obtain the enzymatic hydrolyzate product. Under these conditions, hydrolysates with degrees of hydrolysis of 5%, 10% and 15% were prepared. The hydrolyzate was cooled and freeze-dried to obtain the product. The soluble nitrogen contents of DH5, 10 and 15% TCA were determined to be 54.43%, 64.70% and 76.40%, respectively, and the DPPH clearance rates were 33.88%, 54.49% and 69.48%, respectively.

Embodiment 3

[0043] The mass concentration of casein substrate is 5%, and the hydrolysis temperature is controlled by circulating water bath to 50±0.2°C. According to the ratio of enzyme to substrate protein (E / S) 3 / 100, alkaline protease is added, and by adding 0.1M Standard alkali controls the pH to 8.5±0.1 to reach the corresponding degree of hydrolysis (DH), heats at 95ºC for 10 minutes to inactivate the enzyme, and dries to obtain the enzymatic hydrolyzate product. Under these conditions, hydrolysates with degrees of hydrolysis of 5%, 10% and 15% were prepared. The hydrolyzate was cooled and freeze-dried to obtain the product. The soluble nitrogen content of TCA of DH5%, 10% and 15% was determined to be 53.77%, 64.48% and 76.1%, respectively, and the DPPH clearance rate was 33.89%, 54.42% and 69.15%, respectively.

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Abstract

The invention provides a method for preparing casein hydrolysate with stable functionality by using different hydrolysis conditions, and belongs to the fields of casein hydrolysis technology and processing. The method comprises the following steps: firstly selecting a substrate (casein)-enzyme (alkaline protease) serving as a reaction system, controlling the constancy of pH in a hydrolytic process by using a pH-stat method, changing three hydrolysis conditions, namely temperature (T), enzyme-to-substrate ratio (E / S) and substrate concentration (S) in a certain range, and when hydrolysate reaches established degree of hydrolysis (DH), carrying out enzyme deactivation drying, wherein the properties of the obtained hydrolysate with the same degree of hydrolysis have no significant difference, namely the obtained hydrolysate is stable. According to the method, the hydrolysate with the same degree of hydrolysis is obtained by adjustment according to different production conditions and hydrolysis, so that the hydrolysate has identical properties. The method can be used for guiding industrial production; the production conditions are flexibly changed; and the stability of the products still can be ensured.

Description

technical field [0001] The invention relates to a method for preparing a hydrolyzate with specific functional properties by combining different hydrolysis temperatures and enzyme-to-substrate ratios, and belongs to the field of casein hydrolysis technology and processing. Background technique [0002] Protease is a kind of hydrolase, which mainly hydrolyzes the peptide bonds of proteins and polypeptides. According to the source of the enzyme, proteases can be divided into animal-derived proteases, plant-derived proteases, and microbial-derived proteases. According to the different catalytic positions, proteases can be divided into endopeptidases and exopeptidases. Endopeptidase is an enzyme that is widely used today, and endopeptidase can be divided into four types according to the active site of action: serine protease, cysteine ​​protease, metalloprotease and asparagine protease. Humans have used protease as early as hundreds of years ago, and the typical example is ferm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 孔祥珍叶挺华欲飞张彩猛陈业明孙灵湘
Owner JIANGNAN UNIV
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