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A Photocatalytic Chiral Recognition Method of Amino Acid Enantiomers

A photoelectric catalysis and chiral recognition technology, which is applied in the field of photocatalytic chiral recognition of amino acid enantiomers, can solve problems such as the influence of imprinted expression reproducibility, surface unevenness, spatial structure differences, etc., and achieve a simple and sensitive test process, The effect of stable electrode surface and long service life

Inactive Publication Date: 2016-04-06
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, common inorganic photocatalysts (TiO 2 , ZnO) usually exists in the form of disordered nano-films or particles, the surface is often uneven, three-dimensional undulations; and TiO 2 Photocatalysts are often grown in the mixed crystal form and polycrystalline structure of anatase and rutile
Direct preparation of recognition sites for target contaminants on its surface may have some impact on the reproducibility of blot expression
Moreover, the molecular weight and size of enantiomers of chiral pollutants are exactly the same, and there are only differences in spatial structure, which is a great challenge for the construction of selective photocatalysts with chiral recognition function.

Method used

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  • A Photocatalytic Chiral Recognition Method of Amino Acid Enantiomers

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Experimental program
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Embodiment 1

[0026] The electrode substrate TiO used in the present invention 2 NTs is referred to MaggiePaulose et al. in the literature (AnodicGrowthofHighlyOrderedTiO 2 NanotubeArraysto134nminLength, MaggiePaulose, Karthik Shankaretal, J.Phys.Chem.B, 2006, 110, 16179-16184) prepared by the anodic oxidation method reported in, and corresponding improvements were made. Specifically, the polished and cleaned Ti plate is The anode, the Pt sheet as the cathode, the distance between the electrodes is maintained at 1cm, the voltage is 20V, and the pre-oxidation is carried out in an aqueous solution containing 0.5% HF for 1h. Then in the same system, change to 0.25wt%NH 4 F, 1wt%H 2 o 2 The polyethylene glycol solution is used as the electrolyte, and the anodic oxidation is continued for 2 hours. TiO obtained by anodic oxidation 2 NTs were sintered at 500 °C in an oxygen atmosphere with a heating rate of 1 °C / min.

[0027] TiO prepared by the above method 2 NTs as the substrate, then 5mm...

Embodiment 2

[0029] An electrochemical workstation (CHI660C, Shanghai Chenhua) was used, and a UV lamp with a light intensity of 5 mW was used as the light source, and the distance between the electrode and the light source was controlled to be 3 cm for each experiment. The standard three-electrode system was adopted, with each prepared electrode as the working electrode, the platinum electrode as the counter electrode, the saturated calomel electrode (SCE) as the reference electrode, and the illuminated geometric area of ​​the control electrode was 13×15mm 2 . Using the current-time (i-t) method under light conditions, at 0.1MNa 2 SO 4 It is carried out in the solution, and the current corresponding to the electrode is measured when the lamp is turned on and off when the bias voltage of 0.6V is applied. Taking the L-photoanode electrode as an example, add 1×10 -8 After the L-phenylalanine of M and the interfering substance D-phenylalanine are added to the electrolyte, they are stirred ...

Embodiment 3

[0031] Prepare a series of standard solutions of phenylalanine enantiomers with different concentrations of the substance to be tested; add the standard solutions to the electrolytic cell in turn as the electrolyte, stir for 10 minutes, and then let stand; use the i-t curve method, the light source is 5mW UV lamp, and the distance between the control electrode and the light source is 3cm, and a bias voltage of 0.6V is applied to measure the photocurrent. According to the linear relationship between the photocurrent and the standard solution concentration, the working curve is drawn, and the L-photoanode is detected for L-phenylalanine. The linear fitting equation △I / I=1.7664+3.38268log (C×10 12 L·mol -1 ), the correlation coefficient is R 2 =0.99962, the detection range is 4×10 -12 ~4.18×10 -6 mol / L, the detection limit is 4×10 -12 mol / L,. Then, the electrode is used to detect the photocurrent response of the target enantiomer solution with a known concentration, and the ...

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Abstract

The invention relates to a photoelectrocatalysis chiral recognition method of amino acid enantiomer. According to the method, single crystal ZnO with chiral amino acid recognition capability is established by feeding a template molecule, namely, the amino acid enantiomer, in a hydrothermal process, and a modified electrode is used as a photo-anode and is used for high-selectivity sensitive and rapid photoelectrocatalysis recognition detection on phenylalanine enantiomer. Compared with the prior art, the method combines a high-sensitivity photoelectrocatalysis oxidation technology with the single crystal ZnO photo-anode with a chiral amino acid shape-selective recognition site for identifying and detecting the amino acid enantiomer. The method is simple, low in cost and good in stability and reproducibility, and has the limit of detection reaching up to 10<-12>mol. L<-1>; the photo-anode is good in selectivity while having high sensitivity, and is still capable of specifically identifying the target amino acid enantiomer under the interference of another enantiomer with the concentration of 50 times.

Description

technical field [0001] The invention relates to the technical fields of nanomaterials, photoelectrochemical analysis and chiral biomolecular recognition, in particular to a photoelectric catalytic chiral recognition method for amino acid enantiomers. Background technique [0002] Chirality is one of the essential properties of nature. Many biomolecules that constitute life in nature, such as amino acids, proteins, polysaccharides, nucleic acids, and enzymes, are mostly chiral. Many chemical substances, including some artificially synthesized amino acids, drugs and pesticides also have chiral structures, and often appear in the form of racemates. In an achiral environment, the chiral enantiomers have nearly identical physicochemical properties and can be considered as the same substance. However, in a chiral environment, especially when chiral compounds interact, chiral enantiomers often show different characteristics, and sometimes even show diametrically opposite propertie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30
Inventor 赵国华陈成史慧杰
Owner TONGJI UNIV