Anaerobic ammonia oxidizing bacteria fixed-type bioactive fillers, and preparation and application thereof

An anaerobic ammonia oxidation and biological activity technology, which is applied in the field of fixed biological active fillers, can solve the problems of unsatisfactory biological active filler effect, poor stability of biological active filler, and easy falling off of embedded bodies, and achieves the effect of substrate mass transfer. Good, strong acid and alkali resistance, strong resistance to water impact

Active Publication Date: 2014-07-30
BEIJING UNIV OF TECH
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Problems solved by technology

After experimental research and comparison, we found that the bioactive fillers prepared based on the traditional filler structure and embedding method did not perform well in the test, and there were many problems such as poor overall stability of the bioactive filler, easy falling off of the embedded body, and water-soluble swelling. question

Method used

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  • Anaerobic ammonia oxidizing bacteria fixed-type bioactive fillers, and preparation and application thereof

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Embodiment Construction

[0028] The present invention will be further specifically described below in conjunction with examples, but the embodiments of the present invention are not limited thereto.

[0029] 1. Treatment of anammox bacteria solution

[0030] The anammox bacterial suspension after enrichment culture was centrifuged and concentrated to obtain a bacterial concentration of 3.0×10 9 1 / mL anammox bacteria concentrate, take out 3L of anammox bacteria concentrate for later use.

[0031] 2. Treatment of anammox bacteria solution

[0032] Take 1.0kg of polyvinyl alcohol in a water bath and heat it to 90°C and dissolve it in 7L of water to obtain a polyvinyl alcohol solution, cool it to 30°C and fully mix it with the concentrated solution of anammox bacteria obtained in step 1, add water to make up to 10L for embedding solution, the concentration of polyvinyl alcohol in the embedding solution was 100 g / L.

[0033] 3. Preparation of embedding carrier

[0034] Lay four layers of polyvinyl alco...

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Abstract

The invention discloses anaerobic ammonia oxidizing bacteria fixed-type bioactive fillers, and preparation and an application thereof, and belongs to the technical field of water treatment. Fillers are each composed of a carrier and an embedding body and are fixed on a prefabricated frame to form the fixed-type bioactive fillers; each carrier is prepared by sewing multiple layers of polyvinyl alcohol non-woven fabrics and one layer of textile fabric; the embedding bodies are formed by boric acid cross-linking immobilization of an embedding liquid; the embedding liquid is formed by mixing an anaerobic ammonia oxidizing bacteria suspension and a polyvinyl alcohol solution. Each carrier is soaked in the embedding liquid, through boric acid secondary cross-linking, the non-woven fabrics and the bacteria embedding body are firmly bound together, the embedding body penetrates through textile fabric meshes to form a riveting fixing structure and does not easily fall off, and the good tensile property of the textile fabric improves the overall stability of the bioactive filler. The prepared bioactive fillers not only solve the problems that anaerobic ammonia oxidizing bacteria are low in concentration, are easy to lose and the like, but also have the advantages of strong strength, good stability, strong water flow impact resistant ability and the like, shorten the reaction start-up time, and have broad application prospects.

Description

technical field [0001] The invention relates to the preparation and application of an anaerobic ammox bacteria immobilized biologically active filler in the field of water treatment, in particular to a fixed biologically active filler with textiles and polyvinyl alcohol non-woven fabrics as carriers. Background technique [0002] Anaerobic ammonium oxidation technology (ANAMMOX) is currently known as the most economical biological denitrification pathway. Studies by Strous et al. at Delft University in the Netherlands have shown that the biological reaction process including cell synthesis in the anaerobic ammonium oxidation process can be expressed as the formula ( 1) [0003] NH 4 + +1.32NO 2 ‐ +0.066HCO 3 ‐ +0.13H + → [0004] 1.02N 2 +0.26NO 3 ‐ +0.066CH 2 o 0.5 N 0.5 +2.03H 2 O(1) [0005] From the biological reaction characteristics of ANAMMOX, it can be seen that compared with the traditional nitrification and denitrification technology, ANAMMOX has th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28C02F3/34
Inventor 杨宏王猛管清坤陈伟鄢琳王小乐吴城锋孟婷陶慕翔尚海源胡希佳赵月兰王玉洁姚仁达
Owner BEIJING UNIV OF TECH
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