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Preparation and application of straight cylindrical bioactive filler for immobilization of sulfate-reducing bacteria based on mesh carrier

A biologically active and sulfate-based technology, which is applied in the direction of fixing on/in the organic carrier, can solve the problems of poor stability of bioactive fillers, difficulty in achieving the expected effect, and easy fall-off of embedded bodies, so as to improve hydrophilicity Sex, reduce loss, avoid the growth of miscellaneous bacteria and the effect

Active Publication Date: 2016-06-01
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After experimental research and comparison, we found that the bioactive fillers prepared based on the traditional filler structure and embedding method were difficult to achieve the desired effect in the test application. and many other issues

Method used

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  • Preparation and application of straight cylindrical bioactive filler for immobilization of sulfate-reducing bacteria based on mesh carrier
  • Preparation and application of straight cylindrical bioactive filler for immobilization of sulfate-reducing bacteria based on mesh carrier
  • Preparation and application of straight cylindrical bioactive filler for immobilization of sulfate-reducing bacteria based on mesh carrier

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Preparation of Mesh Support

[0034] Using polypropylene as the main material, add 6wt% polyvinyl alcohol (PVA) to make a straight cylindrical structure mesh carrier with a diameter of 0.8-1.2cm and a height of 0.8-1.2cm. The mesh shape is square and the wire diameter is 1.0mm.

[0035] 2. Preparation of Sulfate Reducing Bacteria Concentrate

[0036] The suspension of sulfate-reducing bacteria after amplification culture was centrifuged and concentrated to obtain 3.5×10 9 A concentrate of sulfate-reducing bacteria per mL.

[0037] 3. Preparation of embedding solution

[0038] Weigh 5kg of polyvinyl alcohol, add 25L of ultrapure water, heat to 85°C, and dissolve for 15min. Take out the dissolved polyvinyl alcohol solution and stir it evenly, then heat it for 5 minutes, stir it evenly and cool it to 31±1°C. Mix 25L of polyvinyl alcohol solution with the prepared 25L of sulfate-reducing bacteria concentrate, add 1.3kg of calcium carbonate, and stir well to make 50L...

Embodiment 2

[0046] 1. Preparation of Mesh Support

[0047] Using polyethylene as the main material, add 6wt% polyvinyl alcohol (PVA) to make a straight mesh carrier with a diameter of 0.8-1.2cm and a height of 0.8-1.2cm. The mesh shape is square and the wire diameter is 0.5mm .

[0048] 2. Preparation of Sulfate Reducing Bacteria Concentrate

[0049] The suspension of sulfate-reducing bacteria after amplification culture was centrifuged and concentrated to obtain 3.5×10 9 A concentrate of sulfate-reducing bacteria per mL.

[0050] 3. Preparation of embedding solution

[0051] Weigh 5kg of polyvinyl alcohol, add 25L of ultrapure water, heat to 85°C, and dissolve for 15min. Take out the dissolved polyvinyl alcohol solution and stir it evenly, then heat it for 5 minutes, stir it evenly and cool it to 31±1°C. Mix 25L of polyvinyl alcohol solution with the prepared 25L of sulfate-reducing bacteria concentrate, add 1kg of calcium carbonate, and stir well to make 50L of embedding solution. ...

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Abstract

The invention discloses preparation and an application of a sulfate reducing bacteria immobilized straight-tubular bioactive filler based on a reticulate carrier, and belongs to the technical field of water treatment. The straight-tubular bioactive filler comprises two parts: an embedding body and the carrier; the carrier is a reticulate straight-tubular carrier formed by taking polyethylene or polypropylene and the like as a main material, adding polyvinyl alcohol or other hydrophilic materials and carrying out hot melting or plate hot pressing; with a reticulate structure of the carrier, the embedding body can be allowed to penetrate through meshes to form a riveting fixing structure together with the carrier so as to increase the filler overall stability; an embedding liquid is formed by mixing a sulfate reducing bacteria concentrated solution and a polyvinyl alcohol solution; the reticulate carrier is evenly coated with the embedding liquid, and the embedding body is formed after boric acid secondary cross-linking immobilization of the embedding liquid and is combined with the reticulate carrier to obtain the sulfate reducing bacteria bioactive filler. The prepared bioactive filler not only can effectively solve the problems that sulfate reducing bacteria are poor in adhesion ability, easy to lose and the like, but also can improve the processing ability of a reactor and shorten the start-up time.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to the preparation and application of a straight cylindrical biologically active filler based on a mesh carrier for immobilizing sulfate-reducing bacteria. Background technique [0002] In the process of using sulfate-reducing bacteria (Sulfate-Reducing Bacteria, SRB) to treat sulfate wastewater, the number, metabolic activity and ecological factors of functional microorganisms (mainly referring to sulfate-reducing bacteria) directly affect the operation and treatment efficiency of the reactor. . In the anaerobic treatment of sulfate wastewater, there are abundant carbon sources (COD originally in the wastewater or added for treatment) and suitable anaerobic conditions, many microorganisms with similar habitat conditions can gather, so how to maintain SRB The dominant position of the population, suitable ecological factors and substrate competitive advantages are the rese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08
Inventor 杨宏胡希佳陈伟管清坤王猛鄢琳王小乐吴城锋孟婷陶慕翔尚海源赵月兰王玉洁姚仁达
Owner BEIJING UNIV OF TECH
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