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Micrococcus yunnanensis and microbial agents and their application

A microbial agent, the technology of Micrococcus yunnanensis, applied in the field of agricultural biology, can solve the problems of poor effect and achieve the effect of promoting growth

Active Publication Date: 2016-06-08
YANTAI DIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to overcome the defect that the existing microbial strains are still poor in promoting the growth of plants under high-salt conditions, the present invention provides a Micrococcus yunnanensis, the preservation number of which Micrococcus yunnanensis is CGMCCNO.8836

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0019] Wherein, the preparation method of the microbial agent may include: inoculating Micrococcus yunnanensis of CGMCC No. 8836 in a fermentation medium for culturing. Preferably, the number of live bacteria of Micrococcus yunnanensis with preservation number CGMCCNO.8836 can be (1-50)×10 in each gram of the microbial agent obtained by culturing. 7 CFU, more preferably (2-20)×10 7 CFU. Wherein, the cultivation temperature may be 28-35° C., and the cultivation time may be 10-50 hours.

[0020] Preferably, the preparation method of the microbial agent may also include: mixing the material obtained after culturing in the fermentation medium with an inert solid carrier and a binder; the inert solid carrier includes clay, silicon dioxide, silicon At least one of algal earth, kaolin, attapulgite, montmorillonite, bentonite, talc, white carbon black and calcium carbonate; the binder includes maltose and / or dextrin. Preferably, the amount of the inert solid carrier and the binder ...

Embodiment 1

[0026] This example is used to illustrate the cultivation of Micrococcus yunnanensis and the preparation of microbial inoculum of the present invention.

[0027] Micrococcus yunnanensis (Micrococcus yunnanensis) with preservation number CGMCCNO.8836 was inoculated into LB medium (containing 10 g / L of tryptone, 5 g / L of yeast extract and 10 g / L of NaCl), at 30 ° C and 150 rpm Cultivate to bacterial density OD under shaking / min 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0028] Add 100mL of the above-mentioned seed bacteria liquid into 100L of fermentation medium (fermentation medium contains 80g / L of soybean powder, 80g / L of starch, 3g / L of sodium chloride, 3g / L of calcium carbonate, 5g / L of phosphoric acid Potassium dihydrogen and ferrous sulfate of 5g / L), cultivated at 30°C, sampled during the cultivation process and observed by microscope direct counting method, until the number of viable bacteria of Micrococcus yunnanensis per gram of culture sol...

Embodiment 2

[0030] This example is used to illustrate the cultivation of Micrococcus yunnanensis and the preparation of microbial inoculum of the present invention.

[0031] Micrococcus yunnanensis (Micrococcus yunnanensis) with preservation number CGMCCNO.8836 was inoculated into LB medium (containing 10 g / L of tryptone, 5 g / L of yeast extract and 10 g / L of NaCl), at 30 ° C and 150 rpm Cultivate to bacterial density OD under shaking / min 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0032] Add 100mL of the above-mentioned seed bacteria liquid into 100L of fermentation medium (fermentation medium contains 80g / L of soybean powder, 80g / L of starch, 3g / L of sodium chloride, 3g / L of calcium carbonate, 5g / L of phosphoric acid Potassium dihydrogen and ferrous sulfate of 5g / L), cultivated at 30°C, sampled during the cultivation process and observed by microscope direct counting method, until the number of viable bacteria of Micrococcus yunnanensis per gram of culture sol...

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PUM

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Abstract

The invention provides a Yunnan micrococcus with a Preservation No. of CGMCC NO.8836. The invention also provides a microorganism bacterium agent, the microorganism bacterium agent contains a thallus and a culture medium, and the thallus contains the Yunnan micrococcus with a Preservation No. of CGMCC NO.8836. The invention also provides the applications of the Yunnan micrococcus and the microorganism bacterium agent in improving the disease resistance of plants under high-salt conditions. Through the technical scheme, according to the invention, the disease resistance of plants under high-salt conditions can be improved more effectively.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to Micrococcus yunnanensis, a microbial agent and applications thereof. Background technique [0002] The lawn is a grassland that is densely planted with perennial dwarf herbaceous plants, artificially established or maintained and managed, and plays the role of greening and beautifying. It is one of the signs of a country and a city's civilization. It is generally set in front of houses, squares, open spaces and around buildings for viewing, recreation or as a sports venue. According to the purpose, the lawn is divided into: recreational lawn, ornamental lawn, sports field lawn, traffic safety lawn and soil protection lawn. [0003] Turfgrass is the basic composition and functional unit of the lawn. It generally has the characteristics of dense growth, and usually needs to be trimmed to keep the surface smooth. There are more than 100 kinds of lawn grasses used around ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/265
Inventor 赵君才阮志勇赵秉强刘艳伟赵文勇吕昭辉刘慧宋昭王彦伟刘小飞
Owner YANTAI DIYUAN BIOTECH