Method for preparing trehalose

A technology of trehalose and trehalose hydrolase, applied in the direction of fermentation, etc., can solve the problems of long conversion time and improvement of conversion efficiency, and achieve the effect of improving production efficiency

Active Publication Date: 2014-07-30
彭氏(惠州)实业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] The current method is ubiquitous, the conversion time

Method used

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  • Method for preparing trehalose
  • Method for preparing trehalose

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1) Starch gelatinization: Stir the starch with water at 50°C to adjust the pH to 6.4-8; the ratio of starch to water is 1:30.

[0042] 2) Liquefaction: α-amylase is used to catalyze the hydrolysis liquefaction reaction. The activity unit of the α-amylase is 5000U / g, and the dosage is 5-6g α-amylase / 100g starch; the secondary jet liquefaction method is adopted, and the starch The paste concentration is 32%, ph6.5, spraying temperature once is 105°C, and spraying temperature is 125°C twice, spraying pressure is 0.2-0.4mPa, keep warm at 96°C for 30min; inactivate enzyme.

[0043] 3) Saccharification: Add 3000U of isoamylase to each liter of starch hydrolyzate after liquefaction, and inactivate the enzyme after the reaction is completed.

[0044] Add maltooligosaccharide-based trehalose synthase and maltooligosaccharide-based trehalose hydrolase to the obtained hydrolyzate; add 300 U maltooligosaccharide-based trehalose synthase and 600 U hydrolase to each liter of starch h...

Embodiment 2

[0053] 1) Starch gelatinization: Stir the starch with water at 60°C to adjust the pH to 8; the ratio of starch to water is 1:25.

[0054] 2) Liquefaction: use α-amylase to catalyze the hydrolysis liquefaction reaction, the activity unit of the α-amylase is 3000U / g, and the dosage is 5-6g α-amylase / 100g starch; the secondary jet liquefaction method is used, and the starch The paste concentration is 32%, ph6.5, spraying temperature once is 105°C, and spraying temperature is 125°C twice, spraying pressure is 0.2-0.4mPa, keep warm at 100°C for 60min; inactivate the enzyme.

[0055] 3) Saccharification: Add 3000U of isoamylase to each liter of starch hydrolyzate after liquefaction, and inactivate the enzyme after the reaction is completed.

[0056] Add maltooligosaccharide-based trehalose synthase and maltooligosaccharide-based trehalose hydrolase to the obtained hydrolyzate; add 500 U maltooligosaccharide-based trehalose synthase and 500 U hydrolase to each liter of starch hydroly...

Embodiment 3

[0064] 1) Starch gelatinization: Stir the starch with water at 55°C to adjust the pH to 6.4; the ratio of starch to water is 1:29.

[0065] 2) Liquefaction: use α-amylase to catalyze the hydrolysis liquefaction reaction, the activity unit of the α-amylase is 4000U / g, and the dosage is 5-6g α-amylase / 100g starch; the secondary jet liquefaction method is used, and the starch The paste concentration is 32%, ph6.5, spraying temperature once is 105°C, and spraying temperature is 125°C twice, spraying pressure is 0.2-0.4mPa, keep warm at 97°C for 42min; inactivate enzyme.

[0066] 3) Saccharification: Add 3000U of isoamylase to each liter of starch hydrolyzate after liquefaction, and inactivate the enzyme after the reaction is completed.

[0067] Add maltooligosaccharide-based trehalose synthase and maltooligosaccharide-based trehalose hydrolase to the obtained hydrolyzate; add 300 U maltooligosaccharide-based trehalose synthase and 600 U hydrolase per liter of starch hydrolyzate; c...

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Abstract

The invention provides a method for preparing trehalose. The method comprises the following step: with starch as a substrate and producing trehalose by using malt oligosaccharyl trehalose synthase and malt oligosaccharyl trehalose hydrolase. According to the method, the trehalose production efficiency is improved by improving processes of liquifying, saccharifying and the like.

Description

technical field [0001] The invention belongs to the field of preparation of glycosyl compounds, and in particular relates to a method for preparing trehalose. [0002] Background technique [0003] Trehalose (Trehalose) is a non-reducing sugar composed of two glucose molecules bonded by α-1,1 glycosidic bonds through hemiacetal hydroxyl groups. Its molecular formula is C12H22O11 and its relative molecular weight is 378.33. Its structural formula is as follows: [0004] [0005] Trehalose widely exists in bacteria, yeast, fungi, algae, insects and plants. It has a non-specific protective effect on organisms and biological macromolecules. This non-specific protective effect is mainly reflected in the fact that when biological cells are in various stress environments such as starvation, drying, high temperature, low temperature freezing, radiation, high osmotic pressure and toxic reagents, the content of intracellular trehalose rises rapidly, thereby protecting life itsel...

Claims

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Application Information

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IPC IPC(8): C12P19/24C12P19/16C12P19/14C12P19/12
Inventor 彭燕辉顾葵
Owner 彭氏(惠州)实业发展有限公司
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