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Transferring receptor detection kit and preparation method thereof

A detection kit and technology for transferrin, which is applied in the field of transferrin receptor content detection, can solve the problems of short shelf life and open bottle stability period, and the sensitivity and stability of the transferrin receptor kit need to be improved, etc. Achieve the effects of prolonging the stable period of opening the bottle, good sensitivity and prolonging the shelf life

Active Publication Date: 2014-07-30
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sensitivity and stability of the latex-enhanced immunoturbidimetric assay kit for detecting transferrin receptors still need to be improved, and the shelf life and stable period after opening the bottle are relatively short.

Method used

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  • Transferring receptor detection kit and preparation method thereof
  • Transferring receptor detection kit and preparation method thereof
  • Transferring receptor detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of latex microsphere particle solution coated with anti-transferrin receptor antibody:

[0031] (1) Washing latex: Take 0.5 mL of amino latex microsphere particles (purchased from Poly Microspheres) with a particle size of 120 nm and a concentration of 10% (w / v) in a 10 mL centrifuge tube, and add 4.5 mL to the centrifuge tube 0.12 mol / L phosphate (PBS) buffer with pH value of 7.4, centrifuged to obtain latex microsphere particles, washed twice with deionized water, and then used 5 mL of 6 mmol / L pH value of 7.4 Phosphate (PBS) buffer to resuspend latex microsphere particles;

[0032] (2) Preparation of antibody solution: take a certain amount of antibody solution (purchased from Taiwan Abnova Corporation), dilute it with 6mmol / L phosphate buffer, and adjust the pH to 5.0;

[0033] (3) Preparation of EDC solution: Take 10 mg of EDC powder and dissolve it in 10 mL of deionized water to prepare an EDC solution with a concentration of 10 mg / mL;

[0034] (4) Preparatio...

Embodiment 2

[0049] Preparation of latex microsphere particle solution coated with anti-transferrin receptor antibody:

[0050] (1) Washing latex: Take 0.6 mL of amino latex microsphere particles with a particle size of 160 nm and a concentration of 10% (w / v) (purchased from Poly Microspheres) in a 10 mL centrifuge tube, and add 4.4 mL 0.12 to the centrifuge tube mol / L phosphate (PBS) buffer with pH 7.4, centrifuged to obtain latex microsphere particles, washed twice with deionized water, and then used 5mL phosphoric acid with a concentration of 5mmol / L pH 7.4 Resuspend latex microsphere particles in saline (PBS) buffer;

[0051] (2) Preparation of antibody solution: take a certain amount of antibody solution (purchased from Taiwan Abnova Corporation), dilute it with 6mmol / L phosphate buffer, and adjust the pH to 5.0;

[0052] (3) Preparation of EDC solution: Take 10mg of EDC powder and dissolve it in 10mL of deionized water to prepare an EDC solution with a concentration of 10mg / mL;

[0053] (4)...

Embodiment 3

[0068] Preparation of latex microsphere particle solution coated with anti-transferrin receptor antibody:

[0069] (1) Washing latex: Take 0.8 mL of amino latex microsphere particles (purchased from Poly Microspheres) with a particle size of 200 nm and a concentration of 10% (w / v) in a 10 mL centrifuge tube, and add 4.2 mL to the centrifuge tube. 12mol / L phosphate (PBS) buffer with a pH of 7.4, centrifuged to obtain latex microsphere particles, washed twice with deionized water, and then used 5mL phosphoric acid with a concentration of 5mmol / L pH 7.4 Resuspend latex microsphere particles in saline (PBS) buffer;

[0070] (2) Preparation of antibody solution: take a certain amount of antibody solution (purchased from Taiwan Abnova Corporation), dilute it with 6mmol / L phosphate buffer, and adjust the pH to 5.0;

[0071] (3) Preparation of EDC solution: Take 10mg of EDC powder and dissolve it in 10mL of deionized water to prepare an EDC solution with a concentration of 10mg / mL;

[0072] ...

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Abstract

The present invention relates to a transferrin receptor content detection kit, which comprises a reagent I and a reagent II, wherein the components of the reagent I comprise a buffer, a preservative, a chelating agent, a surfactant and water, the components of the reagent II comprise anti-transferrin receptor antibody coated latex microsphere particles, a buffer, a preservative, an auxiliary suspending agent and water, and the particle size of the anti-transferrin receptor antibody coated latex particles is 120-200 nm. The invention further relates to a preparation method for the transferrin receptor content detection kit, wherein the anti-transferrin receptor antibody coated latex microsphere particles are prepared by using a chemical cross-linking method. According to the present invention, the amino group latex microsphere particles with the appropriate particle size are selected and the chemical cross-linking method is adopted to prepare the latex microsphere particles, such that the sensitivity and the stability of the prepared transferrin receptor detection kit are increased, and the shelf life and the bottle opening stable phase are prolonged.

Description

technical field [0001] The invention relates to the field of detection of transferrin receptor content, in particular to a transferrin receptor detection kit and a preparation method. Background technique [0002] Iron is distributed in the body in three different ways: approximately 80% of iron is involved in oxygen transport in hemoglobin and in oxygen storage in myoglobin, and several enzymes in the cellular oxidative and respiratory chains. Excess iron is bound to ferritin and hemosiderin for use, since free iron is toxic to cells. Only about 0.1% of the iron is transported in the blood circulation, they are combined with transferrin, and are transported from the "origin" - the protein that binds iron with physiological functions (for example: hemoglobin synthesized in the bone marrow) to the "storage place" " (eg: ferritin in the liver). In a healthy human being, approximately 1 mg of iron is taken in from food per day and an equivalent amount of iron is lost. Red bl...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/54313G01N33/54393G01N33/68G01N33/723G01N2333/79
Inventor 华权高来祥兵许可沈鹤霄舒芹
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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