A method for extracting and separating fucoxanthin from marine unicellular diatoms
A technology for separating fucoxanthin and fucoxanthin is applied in the field of extraction and separation of fucoxanthin, and can solve the problems of low yield of pigment extraction, low fucoxanthin content, affecting pigment extraction and the like , to achieve the effect of short extraction time, short time and fast separation speed
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Embodiment 1
[0031]The above-mentioned cultivated Phaeodactylum tricornutum (Phaeodactylum tricornutum) algae liquid was divided into centrifuge tubes, balanced and put into a centrifuge for centrifugation, the centrifugal force was 3000g, and the centrifugation time was 5 minutes. After the centrifugation was completed, the supernatant was removed, and the algal mud in each tube was combined and weighed. According to the ratio of 5ml / g, add pre-cooled (0-10 degrees) mixture of methanol and acetone as the extraction solution (methanol: acetone = 1:1 (V / V)), shake fully to make the algae cells fully contact with the extraction solution, It was then placed in an ice-water bath for 5 minutes in the dark. During the leaching process, fill nitrogen with a ventilation rate of 3-7L / min to prevent oxidation. The supernatant was collected by centrifugation, and the precipitate was extracted once again with the extraction solution at a ratio of 5 ml / g. Centrifuge and collect the supernatant. The ...
Embodiment 2
[0037] The cultured Phaeodactylum tricornutum (Phaeodactylum tricornutum) algae liquid was divided into centrifuge tubes, balanced and placed into a centrifuge for centrifugation with a centrifugal force of 3000g and a centrifugation time of 5 minutes. After the centrifugation was completed, the supernatant was removed, and the algal mud in each tube was combined and weighed.
[0038] According to the ratio of 5ml / g, add pre-cooled (0-10 degrees) mixture of methanol and acetone as the extraction solution (methanol: acetone = 1:1 (V / V)), fully shake, so that the algae cells can fully contact with the extraction solution , and then placed in an ice-water bath for 5 minutes in the dark. Nitrogen was used to prevent oxidation during the extraction process. The supernatant was collected by centrifugation, and the precipitate was extracted once again with the extraction solution at a ratio of 5 ml / g. Centrifuge and collect the supernatant. The extract was filtered through a filte...
Embodiment 3
[0046] The cultured Thalassiosirapseudonana algae liquid was divided into centrifuge tubes, balanced and loaded into a centrifuge for centrifugation with a centrifugal force of 3000g and a centrifugation time of 5 minutes. After the centrifugation was completed, the supernatant was removed, and the algal mud in each tube was combined and weighed.
[0047] According to the ratio of 5ml / g, add pre-cooled (0-10 degrees) mixture of methanol and acetone as the extraction solution (methanol: acetone = 1:1 (V / V)), fully shake, so that the algae cells can fully contact with the extraction solution , and then placed in an ice-water bath for 5 minutes in the dark. Nitrogen was used to prevent oxidation during the extraction process. The supernatant was collected by centrifugation, and the precipitate was extracted once again with the extraction solution at a ratio of 5 ml / g. Centrifuge and collect the supernatant. The extract was filtered through a filter membrane with a pore size of...
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