Lobule dwarf type magnolia grandiflora tender stem segment isolated culture method

A technology of in vitro culture and Magnolia japonica, applied in the field of plant tissue culture, can solve the problems of difficult to meet the needs of large-scale production and long cycle, and achieve the effects of fast reproduction speed, excellent quality and high reproduction rate.

Inactive Publication Date: 2014-08-20
上海闵行区苗圃有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the above disclosed technology can improve some performance indicators of plants, the cycle is still long, and it is difficult to meet the needs of large-scale production, and it is aimed at plants such as kenaf, Magnolia Magnolia, which has different growth characteristics and properties with the small-leaf dwarf type Magnolia Magnolia. The difference is that the current research on the cultivation methods of the small-leaf dwarf Magnolia magnolia is still blank.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of method for in vitro cultivation of tender stem section of small-leaf dwarf type Magnolia Magnolia, comprising the following steps:

[0028] 1) Selection and pretreatment of the materials to be inoculated: select the semi-lignified new shoots that germinated in the same year, cut off the flattened leaves, rinse with washing powder three times, and rinse under the tap for 3 hours;

[0029] 2) Disinfection of explants: On the ultra-clean table, first disinfect with 75% (vol) ethanol solution for 1 min, then disinfect with 0.2% (vol) new Jieermin solution for 15 min, and finally use 10% (vol) Disinfect with sodium hypochlorite solution for 15 minutes, rinse with sterile water three times, and blot dry with sterile filter paper;

[0030] 3) Establishment of the sterile line: cut the twigs into 1.3cm-long stem segments on an ultra-clean bench, each stem segment has 1 to 2 stem nodes, remove the tissue necrotic due to disinfection at the incision, and remove the oute...

Embodiment 2

[0042] A kind of method for in vitro cultivation of tender stem section of small-leaf dwarf type Magnolia Magnolia, comprising the following steps:

[0043] 1) Selection and pretreatment of the materials to be inoculated: select the semi-lignified new shoots that germinated in the same year, cut off the flattened leaves, rinse with washing powder three times, and rinse under the tap for 3 hours;

[0044] 2) Disinfection of explants: On the ultra-clean bench, first disinfect with 75% (vol) ethanol solution for 2 minutes, then disinfect with 0.2% (vol) new Jieermin solution for 15 minutes, and finally use 10% (vol) Disinfect with sodium hypochlorite solution for 20 minutes, rinse with sterile water three times, and then dry the water with sterile filter paper;

[0045] 3) Establishment of the aseptic system: Cut the twigs into 2.0cm-long stem segments on an ultra-clean bench, each stem segment has 1 to 2 stem nodes, remove the necrotic tissue at the incision due to disinfection,...

Embodiment 3

[0057] A kind of method for in vitro cultivation of tender stem section of small-leaf dwarf type Magnolia Magnolia, comprising the following steps:

[0058] 1) Selection and pretreatment of the materials to be inoculated: select the semi-lignified new shoots that germinated in the same year, cut off the flattened leaves, rinse with washing powder three times, and rinse under the tap for 3 hours;

[0059] 2) Disinfection of explants: On the ultra-clean table, first disinfect with 75% (vol) ethanol solution for 1 min, then disinfect with 0.2% (vol) new Jieermin solution for 15 min, and finally use 10% (vol) Disinfect with sodium hypochlorite solution for 18 minutes, rinse with sterile water three times, and then dry the water with sterile filter paper;

[0060] 3) Establishment of the aseptic system: cut the twigs into 1.5cm-long stem segments on an ultra-clean bench, each stem segment has 1 to 2 stem nodes, remove the tissue necrotic due to disinfection at the incision, and rem...

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Abstract

The present invention relates to a lobule dwarf type magnolia grandiflora tender stem segment isolated culture method, and belongs to the technical field of plant tissue culture. The method comprises: 1) selecting and pre-treating a material to be inoculated, 2) sterilizing an explant, 3) establishing a sterile system, 4) carrying out induction and proliferation culture on clustered shoots, 5) carrying out strong seedling and rooting culture, and 6) carrying out seedling exercising transplantation on test-tube seedlings. The lobule dwarf type magnolia grandiflora tender stem segment isolated culture method has the following beneficial effects that: the clustered shoot induction rate achieves more than 90%, the rooting rate after the strong seedling and rooting culture achieves more than 96%, the survival rate can achieve more than 96%, advantages of rapid reproduction, high reproduction rate, virus removal, purification rejuvenation and the like are provided, the defects of low reproduction coefficient, long period, seasonal restriction and the like in the prior art are overcome, and the high quality seedling can be produced within a short time in a factory manner so as to meet requirements of rural and urban greening. In addition, the one stem segment is adopted as the sample, tens of thousands of the seedlings can be rapidly propagated within a year in the factory manner, and the quality is excellent.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for in vitro culture of tender stem segments of a small-leaf dwarf type magnolia magnolia. Background technique [0002] Small-leaf dwarf Magnolia Magnolia is a new species of Magnolia Magnolia introduced from abroad in recent years. It is a variety of Magnolia Magnolia. , evergreen in four seasons, strong ornamental, white and elegant flower color, flowering from April to May, strong adaptability, and low temperature resistance of minus 15 degrees. Its seedlings are mainly propagated by conventional methods such as cuttings and grafting (not yet popularized in China), but the cycle is long and the rooting rate is low, which cannot meet the needs of production seedlings. [0003] The induction rate directly reflects the reproduction rate and propagation speed of the plant. The Chinese patent No. 201110372472.6: a method for increasing the induct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陆锦明朱天华汤桂钧
Owner 上海闵行区苗圃有限公司
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