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Liquid chromatography and enzyme electrode combining device and application thereof

A technology of liquid chromatography and enzyme electrode, which is applied in the direction of measuring devices, electrochemical variables of materials, instruments, etc., can solve problems such as the complex composition of traditional Chinese medicine ingredients, achieve application prospects and good effects, improve the quality of Chinese medicinal materials, and strengthen the research on active ingredients Effect

Active Publication Date: 2014-08-27
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The composition of traditional Chinese medicine is complex, and conventional analysis techniques have played a role in the qualitative and quantitative analysis of traditional Chinese medicine. However, there is no analytical method that can simultaneously provide the active information of the composition of traditional Chinese medicine and its components.

Method used

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  • Liquid chromatography and enzyme electrode combining device and application thereof
  • Liquid chromatography and enzyme electrode combining device and application thereof
  • Liquid chromatography and enzyme electrode combining device and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] like figure 1 Shown is a liquid chromatography-enzyme electrode combination device, which includes a chromatographic separation unit, a detector, an organic solvent removal unit, an activity evaluation unit and a computer 12, and the chromatographic separation unit adopts a high performance liquid chromatography separation system or an ultra-high pressure For the liquid chromatography separation system, the detector is a single-wavelength ultraviolet detector, a diode array detector or a fluorescence detector. The chromatographic separation unit includes a mobile phase storage tank 1, a chromatographic pump 2, an injection valve 3 and a liquid chromatography column 4 connected to each other through pipelines in sequence, and the liquid phase chromatographic column 4 is connected to the detector 5 through pipelines . The organic solvent removal unit includes a nitrogen cylinder, a nitrogen pump 7, a heating tube 8 and an evaporation tube 6, the nitrogen cylinder adopts ...

Embodiment 2

[0048] A liquid chromatography-enzyme electrode combination method for online analysis of the activity of traditional Chinese medicine components, the method uses the liquid chromatography-enzyme electrode combination device of Example 1 to perform the operations comprising the following steps:

[0049] 1) Preparation of mobile phase

[0050] Water is used as the aqueous phase, and methanol is used as the organic phase. Take deionized pure water and chromatographic methanol, pass through microporous membranes with a pore size of 0.45μ, respectively, and place the filtrates in solvent bottles for the aqueous phase and the organic phase, respectively, for later use.

[0051] 2) Pretreatment of samples to be tested

[0052] Take the dried Atractylodes macrocephala, crush it, weigh 0.6 g, put it in a 25 mL volumetric flask, add 20 mL of methanol, ultrasonically extract for 20 minutes, take it out, put it at room temperature, add chromatographic methanol to the scale, shake well, ...

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Abstract

The invention relates to a liquid chromatography and enzyme electrode combining device. The device comprises a chromatographic separation unit, a detector, an organic-solvent removing unit, an activity evaluation unit and a computer, wherein the chromatographic separation unit comprises a mobile-phase storage tank, a chromatographic pump, a sampling valve and a liquid chromatography column which are connected in sequence by pipelines; the liquid chromatography column is connected with the detector by the pipeline; the organic-solvent removing unit is used for removing an organic solvent in a sample, and the front end of the organic-solvent removing unit is connected with the detector; the activity evaluation unit comprises a mixer, a buffer-solution storage tank and an enzyme electrode; the mixer is connected with the rear end of the organic-solvent removing unit; the buffer-solution storage tank is connected to the mixer which is connected to the enzyme electrode; the computer is respectively and electrically connected with the detector and the enzyme electrode. The liquid chromatography and enzyme electrode combining device has the advantages that the biological activity of all components in the sample can be provided and analyzed in an online and real-time manner, the analysis on the components in the sample to be detected can be realized and the corresponding biological activity of all the components can be evaluated in an online manner.

Description

technical field [0001] The invention relates to a device belonging to the field of medicines, in particular to a liquid chromatography-enzyme electrode combination device and an online analysis application of Chinese medicine components-activity. Background technique [0002] With the expansion of the scale of the Chinese medicine industry and the development of Chinese medicine research results, great progress has been made in the analysis technology of Chinese medicine. , The integration of new technologies makes it a new system to solve key scientific problems, and it is no longer limited to the analysis of the material composition of traditional Chinese medicine, but more focused on solving the correlation between the material composition and its function. The development of global systems biology (GlobalSystems Biology), which systematically reveals the correlation between changes in chemical substance groups and the spatiotemporal response of biological system response...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N27/26
Inventor 寿旦王娜妮张扬董宇
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE