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A liquid chromatography-enzyme electrode combined device and its application

A liquid chromatography, enzyme electrode technology, applied in the direction of measuring devices, material electrochemical variables, instruments, etc., can solve the problems of complex composition of traditional Chinese medicine components, achieve good application prospects and good effects, improve the quality of traditional Chinese medicine materials, and strengthen the research on effective components. Effect

Active Publication Date: 2016-03-30
ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The composition of traditional Chinese medicine is complex, and conventional analysis techniques have played a role in the qualitative and quantitative analysis of traditional Chinese medicine. However, there is no analytical method that can simultaneously provide the active information of the composition of traditional Chinese medicine and its components.

Method used

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  • A liquid chromatography-enzyme electrode combined device and its application
  • A liquid chromatography-enzyme electrode combined device and its application
  • A liquid chromatography-enzyme electrode combined device and its application

Examples

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Embodiment 1

[0046] Such as figure 1 Shown is a liquid chromatography-enzyme electrode combination device, which includes a chromatographic separation unit, a detector, an organic solvent removal unit, an activity evaluation unit and a computer 12, and the chromatographic separation unit adopts a high performance liquid chromatography separation system or an ultra-high pressure For the liquid chromatography separation system, the detector is a single-wavelength ultraviolet detector, a diode array detector or a fluorescence detector. The chromatographic separation unit includes a mobile phase storage tank 1, a chromatographic pump 2, an injection valve 3 and a liquid chromatography column 4 connected to each other through pipelines in sequence, and the liquid phase chromatographic column 4 is connected to the detector 5 through pipelines . The organic solvent removal unit includes a nitrogen cylinder, a nitrogen pump 7, a heating tube 8 and an evaporation tube 6, the nitrogen cylinder adop...

Embodiment 2

[0048] A liquid chromatography-enzyme electrode combination method for online analysis of the activity of traditional Chinese medicine components, the method uses the liquid chromatography-enzyme electrode combination device of Example 1 to perform the operations comprising the following steps:

[0049] 1) Preparation of mobile phase

[0050] Water is used as the aqueous phase, and methanol is used as the organic phase. Take deionized pure water and chromatographic methanol, pass through microporous membranes with a pore size of 0.45μ, respectively, and place the filtrates in solvent bottles for the aqueous phase and the organic phase, respectively, for later use.

[0051] 2) Pretreatment of samples to be tested

[0052] Take dried Atractylodes macrocephala, crush it, weigh 0.6g, put it in a 25mL volumetric flask, add 20mL of methanol, ultrasonically extract for 20min, take it out, put it at room temperature, add chromatographic methanol to the mark, shake well, and filter th...

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Abstract

The invention relates to a liquid chromatography-enzyme electrode combination device. The device includes a chromatographic separation unit, a detector, an organic solvent removal unit, an activity evaluation unit and a computer. tank, chromatographic pump, sampling valve and liquid chromatography column, the liquid chromatography column is connected to the detector through the pipeline; the organic solvent removal unit is used to remove the organic solvent in the sample, and the front end of the organic solvent removal unit is connected to the detector The activity evaluation unit includes a mixer, a buffer storage tank and an enzyme electrode, the mixer is connected to the rear end of the organic solvent removal unit, the buffer storage tank is connected to the mixer, and the mixer is connected to the enzyme electrode; the computer is electrically connected to the The detector and enzyme electrode described above. The invention can provide on-line and real-time analysis of the biological activity of each component in the sample, realize the analysis of the component composition in the sample to be tested and online evaluation of the corresponding biological activity of each component.

Description

technical field [0001] The invention relates to a device belonging to the field of medicines, in particular to a liquid chromatography-enzyme electrode combination device and an online analysis application of Chinese medicine components-activity. Background technique [0002] With the expansion of the scale of the Chinese medicine industry and the development of Chinese medicine research results, great progress has been made in the analysis technology of Chinese medicine. , The integration of new technologies makes it a new system to solve key scientific problems, and it is no longer limited to the analysis of the material composition of traditional Chinese medicine, but more focused on solving the correlation between the material composition and its function. The development of global systems biology (Global Systems Biology), which systematically reveals the correlation between changes in chemical substance groups and the spatiotemporal response of biological system respons...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N27/26
Inventor 寿旦王娜妮张扬董宇
Owner ZHEJIANG ACAD OF TRADITIONAL CHINESE MEDICINE