Method for quickly starting anaerobic ammoxidation reactor

An anammox, rapid start-up technology, applied in the field of anammox reaction, can solve the problems of slow growth rate and low metabolic rate of anammox bacteria, and achieve increased intracellular hemoglobin content, abundant sources, and stability The effect of denitrification performance

Active Publication Date: 2014-09-03
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These two aspects of research are based on the principle of maximizing the retention rate of anammox bacteria in the reactor. Although the rapid s

Method used

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  • Method for quickly starting anaerobic ammoxidation reactor
  • Method for quickly starting anaerobic ammoxidation reactor
  • Method for quickly starting anaerobic ammoxidation reactor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for quickly starting an anammox reactor, comprising the steps of:

[0031] ① Selection of inoculum sludge: Activated sludge from Dalian Lingshui Sewage Treatment Plant was selected as the seed sludge, and the sludge concentration MLSS in the reactor was 2.54g / L.

[0032] ② Reactor: The reactor is an upflow anammox reactor made of PVC (polyvinyl chloride) material with a working volume of 0.5 liters (inner diameter 5 cm, height 25.5 cm).

[0033] ③Composition and concentration of culture medium:

[0034]

[0035] ④Reactor operating conditions: Control the internal temperature of the reactor to 35±1°C, the pH range is between 7.0 and 8.5, the dissolved oxygen is lower than 0.5mg / L, and it is protected from light.

[0036] ⑤ Reactor operation mode: adopt continuous and uninterrupted operation, and the ratio of sludge to culture solution in the reactor is 1g:10mL. During the start-up period, the hydraulic retention time (HRT) was initially set to 12 hours, the...

Embodiment 2

[0038] Others are the same as in Example 1, except that the concentration of Fe(II) is 0.06mM.

Embodiment 3

[0040] Others are the same as in Example 1, except that the concentration of Fe(II) is 0.09mM.

[0041] Table 1

[0042]

[0043]

[0044] As can be seen from Table 1, adopting this technology can accelerate the enrichment rate of anammox bacteria, shorten the start-up period of anammox reactor by about 30%, and the target bacteria obtained in Example 3 have excellent performance in all aspects. In embodiment 1 and embodiment 2. This result shows that by adjusting the concentration of Fe(II) in the culture medium, the metabolic rate of anammox bacteria can be effectively promoted, which is beneficial to the synthesis of heme c (heme c) in its cells, thereby increasing the synthesis of hydrazine Enzyme (HDH) activity, and finally realize the rapid enrichment of anammox bacteria.

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Abstract

The invention relates to a method for quickly starting an anaerobic ammoxidation reactor, belonging to the field of anaerobic ammoxidation reaction. The method comprises the following step: by using activated sludge as a seed sludge and artificially simulated nitrogenous waste water as a culture solution, continuously operating the reactor for at least 50 days, wherein the artificially simulated nitrogenous waste water is composed of 30-60 mg/L ammonia nitrogen, 30-60 mg/L nitrite nitrogen, 125 mg/L potassium bicarbonate, 54 mg/L potassium dihydrogen phosphate and 0.5-5 mg/L ferrous sulfate. The method is simple to operate and easy to implement, has the advantages of no need of any additional energy consumption and abundant iron element sources, and thus, is a practical efficient environment-friendly microbial enrichment technique.

Description

technical field [0001] The invention relates to a method for rapidly starting an anaerobic ammonium oxidation reactor, belonging to the field of anaerobic ammonium oxidation reaction. Background technique [0002] The rapid start-up of the anammox reactor and the high-efficiency enrichment of anammox bacteria have always been the focus and hotspot in the field of anammox research. Due to the long doubling time of anammox bacteria and the low coefficient of sludge yield, its popularization and application are limited. Many scholars at home and abroad have tried to explore ways to quickly start and enrich anammox bacteria through different methods, and have accumulated a lot of experience that can be used for reference. , Aspects of design and improvement of reactors for enrichment of anammox bacteria. These two aspects of research are based on the principle of maximizing the retention rate of anammox bacteria in the reactor. Although the rapid start-up of the reactor has be...

Claims

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Application Information

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IPC IPC(8): C02F3/28
Inventor 乔森毕贞周集体金若菲
Owner DALIAN UNIV OF TECH
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