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Preparation method of liquid complex enzyme from solid fermentation source and application thereof

A technology of liquid compound enzyme and solid fermentation, applied in the field of feed compound enzyme, can solve the problems of poor use effect and achieve the effect of low feed utilization rate, delivery efficiency and high degradation rate

Active Publication Date: 2014-09-03
颜鹏飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a preparation method and application of a liquid compound enzyme derived from solid fermentation, which solves the problem that the existing liquid compound enzyme is only derived from liquid fermentation and has poor use effect

Method used

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  • Preparation method of liquid complex enzyme from solid fermentation source and application thereof
  • Preparation method of liquid complex enzyme from solid fermentation source and application thereof
  • Preparation method of liquid complex enzyme from solid fermentation source and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Solid-state fermentation of Trichoderma longifolia for the production of cellulase and β-glucanase

[0029] The first step, culture medium (6000g bran, 2000g soybean meal, 8000ml nutrient solution, wherein nutrient solution contains 3% ammonium sulfate (mass fraction), 1.5% potassium dihydrogen phosphate (mass fraction)) is mixed in mixer, then Dispense into cloth bags, sterilize at 121°C for 30 minutes with high-pressure damp heat. After the culture medium is cooled to 25-35°C, pour the culture medium into a blender and then add the prepared Trichoderma elongatus powder and stir evenly. Put it on the koji plate, spread it evenly, with a moderate thickness of 3-5cm, and finally cover it with a damp cloth, turn on the temperature control switch, and control the temperature at 29°C. After 40-60 hours of fermentation, turn the koji and replenish water. The amount of water to be added is 8- 15%, and then fermented for another 72 hours to complete the entire fermentation pro...

Embodiment 2

[0034] Aspergillus oryzae solid fermentation to produce xylanase

[0035] The first step, culture medium (6000g bran, 2000g soybean meal, 8000ml nutrient solution, wherein nutrient solution contains 3% ammonium sulfate (mass fraction), 1.5% potassium dihydrogen phosphate (mass fraction)) is mixed in mixer, then Dispense into cloth bags, sterilize at 121°C for 30 minutes with high-pressure damp heat. After the culture medium is cooled to 25-35°C, pour the culture medium into a blender and then add the prepared Aspergillus oryzae powder and stir evenly. In the koji plate, lay it evenly, with a moderate thickness of 3-5cm. Finally, cover it with a damp cloth, turn on the temperature control switch, and control the temperature at 29°C. After 40-60 hours of fermentation, turn the koji and replenish water. %, and then fermented for another 72 hours to complete the entire fermentation process to obtain solid fermented mash;

[0036] Second step, liquid mash: add buffer solution in s...

Embodiment 3

[0040] Solid state fermentation of Aspergillus niger for production of mannanase and α-galactosidase

[0041] The first step, culture medium (6000g bran, 2000g soybean meal, 8000ml nutrient solution, wherein nutrient solution contains 3% ammonium sulfate (mass fraction), 1.5% potassium dihydrogen phosphate (mass fraction)) is mixed in mixer, then Dispense into cloth bags, sterilize at 121°C for 30 minutes with high pressure damp heat. After the culture medium is cooled to 25-35°C, pour the culture medium into a blender and then add the prepared Aspergillus niger powder and stir evenly. In the koji plate, lay it evenly, with a moderate thickness of 3-5cm. Finally, cover it with a damp cloth, turn on the temperature control switch, and control the temperature at 29°C. After 40-60 hours of fermentation, turn the koji and replenish water. %, and then fermented for another 72 hours to complete the entire fermentation process to obtain solid fermented mash;

[0042]The second step,...

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Abstract

The invention relates to a preparation method of a liquid complex enzyme from a solid fermentation source and an application thereof. The method comprises the following steps: solid fermentation: performing high-pressure moist heat sterilization of a culture medium, adding the prepared strain and fermenting to obtain solid fermented mash; liquid mash preparation: adding a buffer solution into the solid fermented mash to obtain the liquid mash; filtering: filtering the liquid mash to obtain enzyme liquid; ultrafiltration concentration: concentrating the enzyme liquid through ultrafiltration to obtain concentrated enzyme liquid; enzyme activity detection and compounding: measuring the enzyme activity of the concentrated enzyme liquid, calculating the addition amount of each single enzyme according to the formula of the complex enzyme, compounding, and diluting to the regulated level to obtain a semi-finished product of a liquid enzyme; addition of a protective agent: adding a stable protective agent into the semi-finished product of the liquid enzyme to obtain the liquid complex enzyme. The method provided by the invention can guarantee the advantage of the solid-fermentation complex enzyme in high degradation rate and ensure that the enzyme activity is not damaged by high-temperature granulation, thereby greatly improving the enzymolysis efficiency.

Description

technical field [0001] The invention relates to a preparation method and application of a liquid compound enzyme derived from solid fermentation, which belongs to feed compound enzyme. Background technique [0002] Feed compound enzymes are more and more widely used in actual production. Compound enzymes for feed are divided into two types in form: solid compound enzymes and liquid compound enzymes. The solid compound enzyme is directly added to the feed raw materials and mixed for granulation, and the liquid compound enzyme is sprayed on the surface of the feed after the feed is granulated. There are two types of enzyme source production methods: solid fermentation and liquid fermentation. Solid compound enzyme has two production methods at the same time: solid fermentation and liquid fermentation; liquid compound enzyme only comes from liquid fermentation. The use effect of solid fermentation compound enzyme is much better than that of liquid fermentation compound enzym...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N9/40C12N9/28A23K1/165C12R1/885C12R1/69C12R1/685C12R1/07A23K20/189
CPCA23K20/189C12N9/2417C12N9/2437C12N9/244C12N9/2465C12N9/248C12N9/2488C12Y302/01001C12Y302/01006C12Y302/01022
Inventor 颜鹏飞
Owner 颜鹏飞