A method for identifying physiological races of tobacco powdery mildew
A technology for physiological races and powdery mildew, applied in the field of agricultural biology, can solve the problems of poor physiological races, large errors and long time.
Inactive Publication Date: 2016-08-24
TOBACCO RES INST CHIN AGRI SCI ACAD
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This method is a traditional identification method, which has the disadvantages of long time and large error.
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[0057] In order to clarify the physiological races and genetic diversity of tobacco powdery mildew in my country, 33 powdery mildew samples were collected from the main tobacco producing areas of the country, purified and inoculated with 12 tobacco varieties, and the relative disease resistance index was calculated according to the disease index of the inoculated tobacco. The disease resistance types of different tobacco varieties to different tobacco powdery mildew varieties were divided, and 5 varieties with good identification ability, such as CF203, Tangpeng, G140, Jingyehuang, and K346, were selected from 12 tobacco varieties to establish an identification host system. It was preliminarily determined that the collected 33 powdery mildew strains were divided into 4 physiological race types. RAPD analysis was performed on the whole genome DNA of 33 tobacco powdery mildew bacteria. First, 14 primers with clear bands and good repeatability were screened out from 50 random primer...
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A, culturing differential hosts (the differential host systems are flue-cured tobacco varieties CF203, G140, K346 and Jingyehuang and a sun-cured tobacco variety Tangpeng) and purifying and breeding erysiphe cichoracearum DC.; B, performing inoculating, investigating and resistance type division, and inoculating an isolated obtained by purifying erysiphe cichoracearum DC. to the above five differential hosts; C, performing DNA extraction; D, utilizing an S42 oligonucleotide random primer to perform PCR amplification on DNA; E, performing electrophoresis detection on PCR to obtain a result and performing data cluster analysis; and F, analyzing a bioassay result and a data cluster analysis result of PCR amplification DNA obtained by utilizing the S42 oligonucleotide random primer, so as to divide erysiphe cichoracearum DC. into different physiological races. The method helps to make up blanks of differential host systems and identification methods for erysiphe cichoracearum DC. physiological races, provides technological support for research on erysiphe cichoracearum DC., and also establish a theoretical basis for control of erysiphe cichoracearum DC. and breeding for disease resistance.
Description
Technical field: [0001] The invention relates to the field of agricultural biotechnology, in particular to a method for identifying physiological races of tobacco powdery mildew fungus. Background technique: [0002] Tobacco powdery mildew is commonly known as "upper gray", "lower frost" and "upper nitrate". The disease mostly damages mature old leaves and develops from bottom to top. Leaf disease, first is a small round yellowish-brown spot, then white powdery dots appear on the back spot, making the spot powdery, the spot expands, and the white powder covers the whole leaf. dead. When it is serious, it will damage the tender stems, and the diseased stems will also be covered with white powder. The diseased leaves are as thin as paper after baking and lose their economic value. It occurs in various smoking areas in China, and the most common and serious harm is in South China, Southwest China and Central China. In addition to harming tobacco, powdery mildew can also ha...
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IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/6858C12Q2535/139C12Q2565/125C12Q2537/165
Inventor 时焦孟坤张保全徐宜民雒振宁王海波
Owner TOBACCO RES INST CHIN AGRI SCI ACAD