Insecticidal lipid agents isolated from entomopathogenic fungi and uses thereof

A technology of lipids and fungi, applied in the field of insect control, can solve the problems of less biological control agents and uneconomical

Inactive Publication Date: 2014-09-10
BIOTELLIGA HLDG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there have been fewer instances of successful application of biological control agents (BCAs), and the BCA era has not been accepted by most growers and is considered potentially uneconomical

Method used

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  • Insecticidal lipid agents isolated from entomopathogenic fungi and uses thereof
  • Insecticidal lipid agents isolated from entomopathogenic fungi and uses thereof
  • Insecticidal lipid agents isolated from entomopathogenic fungi and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0312] Example 1 - Biological Assay for Insect Control

[0313] This example describes the development of a robust test to determine the insecticidal efficacy of various lipids.

[0314] Criteria: 1) effectiveness, 2) susceptibility, and 3) ease of use were used to develop and evaluate tests for target insects.

[0315] All experiments used the target insect Myzus persicae (green peach aphid, Hemiptera) reared on cabbage plants in a normal room. A sample of the lipid fraction was used to inoculate aphids and transfer the aphids to a cabbage leaf on the surface of a 1% water agar plate using 0.05% Tween80 as a wetting agent between the leaf and the agar . Use aphids of mixed ages, usually between 30-50 petri dishes.

[0316] A hand-held Paasche spray gun was adapted to take microvolumes and was used to nebulize 300 μl of test or control solutions. Subsequently, plates with treated aphids were kept at 20°C, 12h light: 12h dark, and checked daily. Remove dead ones. Inoculat...

Embodiment 2

[0317] Example 2 - Biological Assays for Insect Control

[0318] This example describes the development of a large scale bioassay to determine insecticidal efficacy.

[0319] A test for multiple target insects was developed and evaluated using the criteria: 1) effectiveness, 2) susceptibility, and 3) ease of use. Test 4 insect systems: whitefly, cabbage, mealworm and mosquito.

[0320] 1. Whitefly larvae (Hemiptera)

[0321] Whiteflies are the main target species and there is often a lack of suitable control agents. Whitefly larvae were obtained from Bioforce Ltd (Auckland, New Zealand). Groups of approximately 100 larvae were inoculated by spray tower with samples of the K4B3 lipid fraction and control broth.

[0322] 2. Mealworm (Tenebrio molitor) larvae (Coleoptera)

[0323] Tenebrio molitor was obtained from a biological supplier. Ten larvae were sprayed with K4B3 lipid samples or water controls and monitored for 2 weeks.

[0324] 3. Plutella-DBM (Plutella xylostell...

Embodiment 3

[0330] Example 3 - Identification of Pesticide Lipids

[0331] introduction

[0332] This example describes the identification of insecticide lipids prepared from Beauveria bassiana K4B3.

[0333] method

[0334] Cultures of Beauveria bassiana K4B3 were grown in shake flasks and 200L biofermentors. Freeze-dried samples were extracted with methanol and dried. The residue was dissolved in 50% ethanol and extracted with dichloromethane. Wash the dichloromethane with water to remove unwanted co-extractants. A small portion of the remaining dichloromethane extract was infected, redissolved in methanol, and subjected to MS analysis.

[0335] Fractional distillation was performed on a Strata SI-1 Silica column (Phenomenex). Crude samples were dissolved in a small amount of dichloromethane, loaded onto a Silica column, and used a mixture of isopropanol and dichloromethane (100% dichloromethane to 100% isopropanol in multiple steps) Elution was followed by a mixture of methanol ...

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Abstract

The present invention provides insecticidal lipids together with compositions comprising such lipids and lipid fractions and methods of preparing same. Methods for the biological control of insects, such as phytopathogenic insects, using the lipids or compositions comprising said lipids optionally together with one or more insecticidal or entomopathogenic agents including entomopathogenic fungi, are also provided.

Description

technical field [0001] The present invention relates generally to the field of biology, and more particularly to certain embodiments that relate to lipids (including lipids produced by filamentous fungi), compositions comprising the lipids, and the Lipids and compositions are related to the use of biological control agents. In addition, the present invention also provides methods for controlling insects (including phytopathogenic insects) using the lipids and compositions comprising the lipids. Background technique [0002] The ability to control insects and insect populations is important to human and animal health, agriculture, and broader economic viability. For example, insects are carriers of several important human diseases: mosquitoes are carriers of malaria, West Nile virus disease, and dengue fever; ticks are carriers of rickettsial diseases such as typhus, African tick-bite fever and Lyme disease; and fleas as carriers of the plague. Likewise, plant disease or l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C229/22A01H15/00A01N37/44A01N63/04C07C227/40C12N1/14A01N63/30
CPCC12R1/645C12N1/14C07C227/40A01N37/44C12P7/64A01N63/30C12R2001/645C12N1/145C07C229/22A01N2300/00C12P7/6436
Inventor S·R·福特T·R·格拉瑞C·卡德尔R·范金克尔
Owner BIOTELLIGA HLDG
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