Tissue culturing method of Litsea mollis
A technique for cultivating P. chinensis and tissue culture, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficult and precise operation, high cost of electricity, and large labor costs, and achieves the solution of endophytic bacteria. Serious pollution, high cost of electricity, and serious vitrification
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Embodiment 1
[0037] 1. Preparation of light culture room
[0038] The top and surroundings of the natural light cultivation room are generally made of light-transmitting sunlight panels or glass, which can ensure sufficient illumination. Install a pair of shading nets with 90% shading rate on the top layer, install a pair of shading nets with 50% shading rate on the second layer, and install one deck of shading nets on the top of the cultivation frame (use thicker cloth from April to September). , October-March of the following year can use yarn). Around the cultivation room, a double-layer shade curtain is established, one layer is a thick cloth with a shading rate of 75%, and the other layer is a tulle with a shading rate of 30% (similar to a household double-layer curtain). Through different combinations and matching of the top three layers of shade nets and the surrounding two layers of shade curtains, various light intensities required for our seedling cultivation can be obtained. T...
Embodiment 2
[0050] (1) Preparation of the culture room
[0051] (2) Screening and processing of explant materials
[0052] Select an excellent individual plant, carry out basal cutting or circumcision, and after the basal shoots are produced, pick the shoots in the sunny afternoon to obtain semi-lignified shoots with buds. After cleaning the shoots, use a brush to remove impurities , rinsed with running water for 2 hours and drained, then soaked in alcohol with a mass concentration of 75% for 20 seconds under sterile conditions, rinsed once with sterile water, and transferred to HgCl with a mass concentration of 0.1% 2 Disinfect for 4 minutes, then rinse 5 times with sterile water, drain the water for later use;
[0053] (3) Induction culture
[0054] Trim the shoots to 1cm long stems, cut off the leaves, and insert 1 / 2MS+6-BA1.0mg·L -1 +NAA0.05mg·L -1 +0.7% agar+sugar 30g·L -1 Adventitious bud induction was carried out in the culture medium bottle, placed on the second layer of the ...
Embodiment 3
[0063] (1) Preparation of the culture room
[0064] (2) Screening and processing of explant materials
[0065] Select an excellent individual plant, carry out basal cutting or circumcision, and after the basal shoots are produced, pick the shoots in the sunny afternoon to obtain semi-lignified shoots with buds. After cleaning the shoots, use a brush to remove impurities , rinsed with tap water for 3 hours and drained, then soaked in alcohol with a mass concentration of 75% for 20s under sterile conditions, rinsed once with sterile water, and transferred to HgCl with a mass concentration of 0.1%. 2 Disinfect for 7 minutes, then rinse with sterile water for 5 times, and drain the water for later use;
[0066](3) Induction culture
[0067] Trim the shoots to 1cm long stems, cut off the leaves, and insert 1 / 2MS+6-BA1.0mg·L -1 +NAA0.05mg·L -1 +0.7% agar+sugar 30g·L -1 Adventitious bud induction was carried out in the culture medium bottle, placed on the second layer of the cul...
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