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Preparation method of silk fibroin hydrogel

A technology of silk fibroin and hydrogel, which is applied in the field of preparation of silk fibroin hydrogel, can solve the problem of toxicity of silk fibroin hydrogel, long time required for gelation of silk fibroin aqueous solution, and biocompatibility Poor compatibility and other problems, to achieve the effect of good biocompatibility, convenient operation and simple device

Inactive Publication Date: 2014-09-17
陈昆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a preparation method of silk fibroin hydrogel. Compared with the prior art, the invention can solve the problem that the gelation of silk fibroin aqueous solution takes a long time and the prepared silk fibroin hydrogel has problems. Problems of toxicity and poor biocompatibility

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Add 20 g of waste silk into 1 L of sodium carbonate aqueous solution with a mass fraction of 0.05%, adjust the temperature to 95° C., let it stand for 0.5 hours, filter, collect the obtained filter residue, wash the filter residue fully with water, and dry at 70° C. for 5 hours to obtain Silk fiber: add 5g of the obtained silk fiber to 100mL of calcium chloride aqueous solution with a mass fraction of 40%, adjust the temperature to 98°C, and let it stand for 8 minutes; the solution after the reaction is cooled to room temperature, filtered with gauze, and the filtrate is collected Put the filtrate into a dialysis bag, dialyze it with tap water for 8 hours, and then dialyze it with distilled water for 10 hours; collect the solution after dialysis in the dialysis bag, whip and homogenize it at a speed of 3000r / min for 300s, statically Set aside for 4 hours to obtain silk fibroin hydrogel.

Embodiment 2

[0021] Add 30 g of waste silk into 1 L of sodium carbonate aqueous solution with a mass fraction of 0.05%, adjust the temperature to 98°C, let it stand for 1 hour, filter, collect the filter residue, wash the filter residue with water, and dry it at 80°C for 4 hours to obtain Silk fiber: add 10g of the obtained silk fiber to 100mL of calcium chloride aqueous solution with a mass fraction of 40%, adjust the temperature to 99°C, and let it stand for 7 minutes; cool the reacted solution to room temperature, filter it with gauze, and collect the filtrate Put the filtrate into the dialysis bag, dialyze it with tap water for 9 hours, and then dialyze it with distilled water for 9 hours; collect the solution after dialysis in the dialysis bag, whip and homogenize it at a rate of 7000r / min for 200s, statically Set aside for 2 hours to obtain silk fibroin hydrogel.

Embodiment 3

[0023] Add 40 g of waste silk to 1 L of aqueous sodium carbonate solution with a mass fraction of 0.05%, adjust the temperature to 100°C, let stand for 2 hours, filter, collect the resulting filter residue, wash the filter residue fully with water, and dry it at 85°C for 3 hours to obtain Silk fiber: Add 25g of the obtained silk fiber to 100mL of calcium chloride aqueous solution with a mass fraction of 40%, adjust the temperature to 100°C, and let it stand for 6 minutes; cool the reacted solution to room temperature, filter it with gauze, and collect the filtrate Put the filtrate into the dialysis bag, dialyze it with tap water for 10 hours, then dialyze it with distilled water for 8 hours; collect the solution after dialysis in the dialysis bag, whip and homogenize it at a rate of 10000r / min for 150s, statically Set aside for 2.5 hours to obtain silk fibroin hydrogel.

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Abstract

The invention discloses a preparation method of silk fibroin hydrogel. Waste natural silks are subjected to degumming, dissolution and dialysis treatment so that a low-concentration silk fibroin solution is obtained; next, high-speed stirring homogenization treatment at a certain rate is carried out at normal temperature, and then the silk fibroin hydrogel can be obtained after standing. The method of high-speed stirring homogenization treatment is adopted so that the gelation time of the silk fibroin solution is greatly reduced; the obtained product is green, safe and good in biocompatibility and can be applied to the industries such as food, medical materials and the like.

Description

technical field [0001] The invention belongs to the field of hydrogel materials, in particular to a preparation method of silk fibroin hydrogel. Background technique [0002] Silk fibroin hydrogel is a material between solid and liquid. It has the advantages of non-toxic, non-irritating, good biocompatibility, and good water holding capacity. It can be widely used in food, skin care products, bionic materials, Separation devices, drug release materials and other fields. [0003] Silk fibroin hydrogel can be prepared from silk fibroin solution through natural gelation, but natural gelation takes about one month, which greatly reduces work efficiency. Therefore, can the silk fibroin hydrogel time be shortened? It is one of the key factors for the quality of silk fibroin hydrogel. At present, the main method to shorten the gelation time of silk fibroin hydrogel is to add a chemical cross-linking agent to the silk fibroin aqueous solution. This method can greatly shorten the g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L89/00
Inventor 陈昆
Owner 陈昆
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