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Composite upconversion nanoprobe with multicolor luminescent spectrums and preparation method and application of composite upconversion nanoprobe

A technology of luminescence spectrum and nanoprobe, which is applied in composite upconversion nanoprobe and its preparation method and application field, can solve the problems of background fluorescence interference, complex surface environment of TNT attachment, etc., achieve detection sensitivity and accuracy assurance, overcome Effects of background fluorescence interference and improvement of detection sensitivity

Inactive Publication Date: 2014-09-24
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method has background fluorescence interference, and because of the complex environment of TNT attachment surface in security inspection and other complex environments, it is very necessary to develop a method that can effectively overcome background fluorescence and improve detection sensitivity.

Method used

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  • Composite upconversion nanoprobe with multicolor luminescent spectrums and preparation method and application of composite upconversion nanoprobe
  • Composite upconversion nanoprobe with multicolor luminescent spectrums and preparation method and application of composite upconversion nanoprobe
  • Composite upconversion nanoprobe with multicolor luminescent spectrums and preparation method and application of composite upconversion nanoprobe

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Embodiment 1

[0028] (1) Surface-functionalized upconversion luminescent host nanomaterials with blue fluorescence emission (PEI-coated NaYF doped with Yb and Tm 4 : PEI-NaYF 4 : Yb, Tm) preparation: the main synthetic steps refer to the method reported in the literature [Wang F, Liu X G, Upconversion Multicolor Fine-Tuning: Visible to Near-Infrared Emission from Lanthanide-Doped NaYF 4 Nanoparticles [J]. J. Am. Chem. Soc. 2008, 130 (17): 5642–5643.]. Synthetic PEI-NaYF 4 : Yb, Tm have certain spectral characteristics: the ratio of up-conversion fluorescence emission peaks at 477 nm to 808 nm is 2:1.

[0029] The present invention synthesizes PEI-NaYF with the above spectral properties by adjusting the doping ratio 4 : Yb, Tm nanoparticles. The specific experimental steps are as follows: Use a pipette gun to accurately measure 8.15, 1.8, and 0.05 mL of yttrium chloride, ytterbium chloride, and thulium chloride solutions with a concentration of 0.2 M in a 200 mL beaker; then add 10 mL o...

Embodiment 2

[0033] Embodiment 2: The above composite up-conversion nanoprobe is used for TNT detection

[0034] (1) Optional

[0035] In the experiment of testing the selectivity of TNT based on composite upconversion fluorescent nanoprobes, the explosives 2,4-dinitrobenzene (DNT) and nitrobenzene (NB) which are structurally similar to TNT were used as detection objects to investigate the detection Probe selectivity: Take 10 μL of 2,4-dinitrobenzene and nitrobenzene at a concentration of 1 mM and 1 mg / mL fluorescent probe in a centrifuge tube, and then dilute with water to a TNT analog concentration of 40 μΜ. The reaction solution was subjected to ultraviolet-visible spectrum detection (see attached figure 2 ), it can be seen from the ultraviolet absorption spectrum that only the solution added with TNT has absorption at 450 nm, and it can be observed that only the solution added with TNT turns orange-yellow, which shows that only TNT can selectively interact with the probe Amino grou...

Embodiment 3

[0038] Embodiment 3: the preparation of TNT detection test paper

[0039] Cut clean white cardboard into small pieces of 5×5 cm as the adsorption substrate, and then glue 6×6 mm waterproof gaskets on the white cardboard pieces to form a 5×5 array, in which every two waterproof The spacing of the gaskets is 4mm; then a filter paper disk with a diameter of 5mm for detection is pasted on the waterproof gasket to form a detection area; the concentration is 0.1 mg mL -1 The composite up-conversion nanoprobes were evenly added dropwise onto the above-mentioned filter paper disc (2 μL), and dried at 60 °C for 1 hour, so that the composite up-conversion nanoprobes were evenly fixed on the filter paper disc to form a detection area (see image 3 ). The TNT detection test paper is simple and convenient to make, low in cost, easy to use and convenient for mass production.

[0040] Drop 1 μL of TNT solution with a concentration of 40 μM on the test paper, and then irradiate it with a 98...

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Abstract

The invention discloses a composite upconversion nanoprobe with multicolor luminescent spectrums and a preparation method and an application of the composite upconversion nanoprobe. PEI-NaYF4:Yb,Tm and PAA-NaYF4:Yb,Er of which the mass ratio is 3:1 are evenly mixed, so that the composite upconversion nanoprobe with the multicolor luminescent spectrums is obtained. The nanoprobe is made of a rare-earth-based upconversion luminescent nano-material and has the multiple visible luminescent zone spectrums, and therefore proportional fluorescence visual detection can be achieved. According to TNT test paper prepared by using the nanoprobe as a host material, under excitation by a light source with the wavelength being 980 nm, whether TNT exists or not is judged by observing whether a fluorescence color of the test paper changes or not. The upconversion luminescent nano-material is excited on the basis of the near-infrared light source, background fluorescence interference can be well overcome, and therefore detection sensitivity can be improved to a great extent. A synthetic method of the host material is simple and practicable, a substrate of the test paper is low in price, the TNT test paper and the excitation light source thereof are convenient to carry, operation is simple, reliability is high, and therefore the TNT test paper is very suitable for a security check site.

Description

technical field [0001] The invention relates to a composite up-conversion nano-probe with multicolor luminescence spectrum, a preparation method and application thereof, and belongs to the field of chemical detection. technical background [0002] According to media reports, various terrorist attacks involving explosions have occurred frequently in the world recently. Due to its high explosive energy and low price, trinitrotoluene (TNT) is often used by terrorists as explosives. Usually terrorists hide explosives in secret places and tie them to hostages or themselves, which brings great difficulties to the timely detection of TNT. However, even if the explosives are carefully concealed, there will still be a very small amount of TNT remaining on the packaging bag wrapped in explosives or on the clothes of the carrier, so it is urgent to develop a method that can easily detect a small amount of TNT on-site. [0003] In recent years, although TNT detection technology has ma...

Claims

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Application Information

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IPC IPC(8): C09K11/85C09K11/02G01N21/64
Inventor 袁荃胡晓霞魏婷王杰
Owner WUHAN UNIV
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