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Construction method of Jinhua pig ear dermis desmocyte system

A technology of fibroblasts and construction method, which is applied in the construction field of Jinhua pig ear dermal fibroblast cell line, and achieves the effects of avoiding damage, high viability and high purity

Inactive Publication Date: 2014-09-24
JINHUA ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many scholars have carried out quantitative research on the culture of animal dermal fibroblasts, there are relatively few reports on the in vitro culture of porcine dermal fibroblasts, especially the establishment of pig ear fibroblast cell lines (strains).

Method used

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  • Construction method of Jinhua pig ear dermis desmocyte system
  • Construction method of Jinhua pig ear dermis desmocyte system

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The present invention takes the construction of the Jinhua pig ear dermal fibroblast cell line as an example in detail as follows;

[0021] The biological materials involved in the present invention are obtained from ordinary Jinhua pigs, and the biological materials that must be used to complete the invention are readily available to the public, so no preservation is required.

[0022] The selection of experimental reagent is very important among the present invention, and main experimental reagent is:

[0023] DMEM (Dulbecco's Modium Essential Medium) medium is the product of Hyclone; fetal bovine serum (FBS); penicillin and streptomycin mixture is the product of Hyclone (including penicillin 10000u / ml and streptomycin 10ug / ml); 0.25% trypsin solution is a product of Hyclone Company; Giemsa stain (Giemsa stain) is a product of Fluka Company; tylosin (tylosin) is a product of Sigma Company; PBS solution is a product of Hyclone Company.

[0024] Complete culture medium: ...

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Abstract

The invention aims to provide a construction method of a Jinhua pig ear dermis desmocyte system, which is convenient for extracting a stable and pollution-free cell system. For realizing the aim, the construction method of the Jinhua pig ear dermis desmocyte system comprises a living body collection step, a separation and culture step and the like for culture. By adopting the technical scheme, the construction method disclosed by the invention can be used for successfully starting primary culture of the pig ear dermis desmocyte system by adopting a method in which enzymic digestion is combined with primary explantation, thus the Jinhua pig ear dermis desmocyte system is successfully constructed.

Description

technical field [0001] The invention relates to a method for constructing a Jinhua pig ear dermal fibroblast cell line, and belongs to the field of cell biology and biotechnology. Background technique [0002] my country is rich in livestock and poultry genetic resources. There are 20 species of pigs, chickens, ducks, geese, special poultry, cattle, buffaloes, yaks, Dulong cattle, sheep, goats, etc., with a total of 576 varieties and groups, of which the local breeds (groups) are There are 426 varieties, 73 cultivated varieties and 77 introduced varieties. In the past 20 years, 41.9% of the populations of livestock and poultry genetic resources in my country have declined to varying degrees. The reason is that on the one hand, people have insufficient understanding of the resource characteristics of local breeds. On the other hand, the production performance of some local breeds cannot meet the needs of the current market. This has led to a decline in the number of some loc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12R1/91
Inventor 项云屠平光章啸君楼芳芳杜喜忠胡旭进
Owner JINHUA ACAD OF AGRI SCI
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