Multiparameter integrated chromatographic flow-through cell

Abstract

The invention relates to the field of chromatographic techniques, specifically to a multiparameter integrated chromatographic flow-through cell. The multiparameter integrated chromatographic flow-through cell comprises a cell body (1), a conductivity electrode (2), a pH electrode (3), a temperature compensation electrode (4), a to-be-tested liquid path (5) and two quartz plates (6), wherein the conductivity electrode (2) and the pH electrode (3) are respectively located at two ends of the top of the cell body (1), the to-be-tested liquid path (5) is located below the conductivity electrode (2) and the pH electrode (3) and passes through the cell body (1), and the two quartz plates (6) allow ultraviolet-visible light to pass through and are respectively arranged at two sides of the central position of the to-be-tested liquid path (5) to form an ultraviolet-visible light absorption value test optical path. The invention has the following advantages: no extra pipeline is needed for connection of each electrode and the optical path; the multiparameter integrated chromatographic flow-through cell has a simple and compact structure and a small size, effectively avoids problems like non-synchronization of measuring time, easy leakage of a to-be-measured liquid, etc. and is applicable to fields of chromatographic separation or purification.

Description

technical field [0001] The invention relates to the technical field of chromatography, in particular, the invention relates to a multi-parameter integrated chromatography flow cell. Background technique [0002] In chromatographic separation or purification, it is necessary to simultaneously measure the UV-visible light absorption, pH value, conductivity and temperature of the chromatographic effluent. Especially the chromatographic separation or purification of proteins, mainly through one or more methods of ion exchange, hydrophobic interaction, metal chelation, affinity and gel filtration chromatography, the target protein and impurity molecules are separated by molecular weight, charge, hydrophobicity, etc. To separate the differences in various properties such as sex, ligand affinity, etc., it is necessary to use an ultraviolet detector to monitor the target molecules and impurities online, and the pH value, conductivity and temperature of the chromatographic mobile pha...

AI Technical Summary

Problems solved by technology

Chinese patent 200420085568.X discloses a multi-parameter electrode flow cell for water quality detection. The conductivity electrode, dissolved oxygen electrode, temperature electrode and pH electrode are installed in the electrode flow cell of a rectangular box. However, this flow cell The internal volume is large, and there is no ultraviolet flow cell, so it is not suitable for online chromatography detection; US patent US5420432A discloses an organic pollutant detection device, which integrates pH, conductivity, temperature, ultraviolet absorption and other detection methods in one cavity ,, and apertures, optical lenses, and ultraviolet interference optical filters must be installed on both sides of the cavity for ultraviolet absorption detection. The structure is complex and takes up a lot of space, so this device cannot be applied to chromatographic separation or purification.

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