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Method for extracting and separating sinensetin

A separation method, the technology of aurantrone, which is applied in the field of extraction and separation of arurinone, can solve the problem of not finding arurinone, etc., and achieve the effect of large preparation amount and high product content

Inactive Publication Date: 2014-10-01
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no report on the method of preparing orange flavone in large quantities in China.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Grind the aerial part of Chinese quinoa, pass through a 20-mesh sieve, weigh 1 kg and put it into an extraction tank, weigh 2.2 g of cellulase and dissolve it in 7 L of pH4. Heat up to 80°C to inactivate the enzyme, filter, add 10 times the amount of 70% ethanol solution to the enzymolysis raw material, microwave extraction 3 times at 50°C, filter to obtain the extract, concentrate under reduced pressure to extract, add water to disperse, add NK- 9 Adsorbed on a macroporous adsorption resin column, eluted with water first, then eluted with 3BV50% ethanol, collected the eluate and concentrated under reduced pressure to obtain the extract. Dissolve the extract in methanol, inject it into a preparative high-performance liquid chromatograph, use acetonitrile-water (30:70) as the mobile phase, and detect on-line with ultraviolet light, collect high-concentration fractions, concentrate by rotary evaporation, and dry at low temperature to obtain 1.1 g of orange flavone , conte...

Embodiment 2

[0019] Grind the aboveground part of Chinese quinoa, pass through a 20-mesh sieve, weigh 1 kg and put it into an extraction tank, weigh 2.8 g of pectinase and dissolve it in 7 L of pH4. Heat up to 80°C to inactivate the enzyme, filter, add 6 times the amount of 60% ethanol solution to the enzymolysis raw material, ultrasonically extract 3 times at 50°C, filter to obtain the extract, concentrate under reduced pressure to extract, add water to disperse, add ADS- Adsorbed on a 21 macroporous adsorption resin column, first eluted with water, then eluted with 4BV60% ethanol, collected the eluate and concentrated under reduced pressure to obtain the extract. Dissolve the extract in methanol, inject it into a preparative high-performance liquid chromatograph, use acetonitrile-water (33:67) as the mobile phase, and detect on-line with ultraviolet rays, collect high-concentration fractions, concentrate by rotary evaporation, and dry at low temperature to obtain 0.97 g of orange flavone ...

Embodiment 3

[0021] Crush the aboveground part of Chinese quinoa, pass through a 20-mesh sieve, weigh 3kg and put it into the extraction tank, weigh 12.6g of pectinase and cellulose compound enzyme (ratio 1:2), dissolve it in 7L of pH4.5 aqueous solution and add it to the extraction tank , Enzymolysis at 50°C for 9 hours, heating to 80°C to inactivate the enzyme, filtering, adding 8 times the amount of 75% ethanol solution to the enzymolysis raw material, microwave extraction twice at 50°C, filtering to obtain the extract, and reducing pressure Concentrate to extract, add water to disperse, add NK-9 macroporous adsorption resin column for adsorption, first elute with water, then take 3BV70% ethanol to elute, collect eluent and concentrate under reduced pressure to obtain extract. Dissolve the extract in methanol, inject it into a preparative high-performance liquid chromatograph, use acetonitrile-water (35:65) as the mobile phase, and detect on-line with ultraviolet rays, collect high-conce...

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PUM

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Abstract

The invention discloses a method for extracting and separating sinensetin. The method is characterized by comprising the following steps: crushing ground part of dysphania botrys, adding a biological enzyme for performing enzymolysis for 8-10 hours, extracting enzymolysis raw material for 2-3 times through a microwave extraction process or ultrasonic extraction process by adding a 60-80% ethanol solution, concentrating an extraction solution till an extract is formed, adding a proper amount of water for dispersing the extract, subsequently adding the extract into a macroporous resin column for absorbing the extract, and then separating the extract by using a preparative high-performance liquid chromatography to obtain the sinensetin. The method is large in preparation quantity, high in content of products and suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of extracting effective components of natural plants, and relates to a method for extracting and separating orange flavonoids. Background technique [0002] Aridanin is a flavonoid compound extracted from the leaves of Chenopodium botrys L. in the family Chenopodiaceae. Molecular formula is C 20 h 20 NO 7 , molecular weight 372.37, mp.169-171°C. Aurantone has the activity of inducing differentiation of mouse myeloid leukemia cells. When the concentration is 50μM and 5μM, the growth rate is 62% and 81% (control 100%), and the activity of phagocytic cells is >10%. The concentration is 100μM and 50μM When the concentration of HL-60 cells was 50% and 73%, the activity of phagocytic cells was >25% and >10% respectively; anti-tumor effect, when the concentration was 30 μg / mL, the growth rate of Ehrlich’s ascites tumor cells was inhibited in vitro, and the growth inhibition rate It inhibits the releas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH