Extraction method of herba artemisiae annuae volatile oil, and product and application of herba artemisiae annuae volatile oil

A technology for volatile oil of Artemisia annua and an extraction method, which is applied in the field of plant pesticides, can solve problems such as failure to be effectively utilized, and achieve the effects of good insecticidal activity, easy availability of raw materials, and improved utilization rate.

Active Publication Date: 2014-10-01
SHENYANG RES INST OF CHEM IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to the research on the extract of Artemisia annua in the prior art, the main active ingredients are still mostly related to artemisinin, and the residue left after extracting artemisinin is usually burned as fuel or buried as waste, and has not been effectively utilized.

Method used

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  • Extraction method of herba artemisiae annuae volatile oil, and product and application of herba artemisiae annuae volatile oil
  • Extraction method of herba artemisiae annuae volatile oil, and product and application of herba artemisiae annuae volatile oil
  • Extraction method of herba artemisiae annuae volatile oil, and product and application of herba artemisiae annuae volatile oil

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Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: Extraction of Artemisia annua volatile oil

[0021] The raw material of artemisinin oil comes from: Zhangjiagang Swisson Biomedical Engineering Co., Ltd., the remaining waste of artemisinin extraction process, and the content of artemisinin is less than 1%.

[0022] Artemisia annua oil and activated carbon were added to the extractor at a mass ratio of 1:1. Under the following extraction conditions, the volatile oil of Artemisia annua was obtained as light yellow semi-solid.

[0023] The extraction temperature is 45-55°C, the extraction pressure is 20-25MPa; the temperature of the separation tank is 45-55°C, and the pressure of the separation tank is 7-8MPa; CO 2 The flow rate is 15-20L / h; the extraction time is 120min, if no volatile components come out, stop the extraction.

Embodiment 2

[0024] Embodiment 2: LC-MS analysis of Artemisia annua volatile oil

[0025] Conditions: DB-5 capillary column (30m×0.25mm×0.25um); carrier gas He, flow rate 1.0mL / min; split ratio 10:1; inlet temperature 280°C; The temperature was raised to 70°C at 4°C / min, kept for 10 minutes, and then raised to 280°C at 6°C / min; the injection volume was 0.1uL.

[0026] The mass spectrometry conditions adopt EI mode, the electron energy is 70eV; the ion source concentration is 200°C; the scanning range is 30-600amu, and the interval is 0.5s.

[0027] The following table lists the components in the volatile oil of Artemisia annua with a content exceeding 0.25%:

[0028]

[0029]

Embodiment 3

[0030] Embodiment 3: insecticidal activity test

[0031] 1. Determination of insecticidal activity of Lepidoptera larvae:

[0032] The artificial feed film method was used.

[0033] The basic operation is as follows: Prepare a sufficient amount of 24-well culture plate with 1ml of artificial feed per well, add liquid medicine on the surface of artificial feed to form a drug film, add 0.05ml of liquid medicine to each well, and inoculate worms after the liquid is dry, one head per well Test worms, each concentration is a treatment, a total of 24 test worms on a culture plate for each treatment. After the treatment, put them into the observation room, and observe the death of the test insects regularly. After 7 days, check and record the number of dead heads of the test insects, and calculate the mortality rate.

[0034] 2. Determination of insecticidal activity of green peach aphid and spider mite cinnabarinus:

[0035] It is carried out by plant living spray method.

[...

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Abstract

The invention relates to the technical field of plant insecticides and specifically discloses an extraction method of herba artemisiae annuae volatile oil, the prepared herba artemisiae annuae volatile oil and application of the herba artemisiae annuae volatile oil. The extraction method is characterized by taking the artemisia annuae oil as the raw material and extracting the artemisia annuae volatile oil by using CO2 supercritical extraction method so as to obtain the herba artemisiae annuae volatile oil with volatility, wherein the artemisia annuae oil is residue obtained after extracting artemisinin from the artemisia annuae oil. The extraction method is simple and low in cost of raw material; moreover, the utilization rate of the artemisia annua is improved; the obtained herba artemisiae annuae volatile oil is good in insecticidal activity and can be used as an effective component for killing insects in agriculture.

Description

technical field [0001] The invention relates to the technical field of plant pesticides, in particular to a method for extracting volatile oil of Artemisia annua, the obtained product and its application. Background technique [0002] Artemisia annua is the above-ground part of Artemisia annua, which has the functions of clearing heat and relieving heat, removing steam, and antimalarial. Its main ingredient, artemisinin, is a sesquiterpene lactone, which is highly effective, quick-acting, low-toxic, and safe in the treatment of dangerous cerebral malaria. It is also the only new antimalarial drug that has been internationally recognized in my country so far. [0003] Plant-derived insecticides are popular because they do not have the problems of resistance, residue, and pollution caused by conventional chemical pesticides. The use of botanical insecticides containing components of Artemisia annua, its extract, has been reported. However, according to the research on Artemi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02A01N65/12A01P7/02A01P7/04
Inventor 李旭王远焦姣宋玉泉沈丽红王洪庆
Owner SHENYANG RES INST OF CHEM IND
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