Kit used for detecting number of human chromosomes 21
A technology for the number of chromosomes and detection reagents, applied in the field of molecular biology, can solve the problems of DNA quality difference, inaccuracy of results, annealing temperature changes, etc.
Inactive Publication Date: 2014-10-08
钦州市妇幼保健院
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The invention discloses a kit used for detecting the number of human chromosomes 21. The kit comprises an amplification detection reagent, wherein the amplification detection reagent comprises a primer pair used for simultaneously amplifying specific repetitive sequences of chromosomes 21 and specific repetitive sequences of chromosomes 11, a fluorescent probe used for specifically detecting the specific repetitive sequences of the chromosomes 21 and a fluorescent probe used for specifically detecting the specific repetitive sequences of the chromosomes 11. The kit has the beneficial effects that the kit adopts a pair of primers to simultaneously amplify a pair of similar sequences between different chromosomes, thus avoiding mutual interference or other interferences caused by amplifying different sequences by different primers and ensuring the consistency of the amplification efficiency; the fluorescent probes are selectively designed in the DNA sequence sites with base difference so that the amplification volumes of two sequences can be respectively detected accurately via deltaCT values of real-time fluorescent PCR (polymerase chain reaction) of repeat segments, thus further improving the accuracy and reliability of the detection results and reducing the probability of causing false negative or false positive results.
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