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Method of detecting a salmonella microorganism

A technology for Salmonella and microorganisms, which is applied in the field of compositions with or without existence, and can solve the problems such as the difficulty in detecting Salmonella microorganisms

Inactive Publication Date: 2014-10-08
3M INNOVATIVE PROPERTIES CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the presence of relatively low numbers of Salmonella microorganisms in the sample, the presence of relatively high numbers of closely related non-Salmonella enteric microorganisms in the sample, and / or non-microbial substances in the sample that can interfere with the growth or detection of Salmonella microorganisms ( For example, the presence of food particles or soluble chemicals), the detection of Salmonella microorganisms in food samples can be difficult

Method used

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  • Method of detecting a salmonella microorganism
  • Method of detecting a salmonella microorganism
  • Method of detecting a salmonella microorganism

Examples

Experimental program
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Effect test

Embodiment 1

[0085] Embodiment 1 is a composition comprising:

[0086] semi-solid medium comprising:

[0087] gelling agent;

[0088] at least one first selection agent that inhibits the growth of Gram-positive microorganisms;

[0089] a first differential indicator system comprising at least one first differential indicator compound; and

[0090] a second differential indicator system comprising a second differential indicator compound that is converted by urease activity to a second detectable product;

[0091] wherein said first differential indicator compound is capable of being converted by members of the genus Salmonella into a first detectable product;

[0092] wherein said first differential indicator compound is not capable of being transformed by genera of non-Salmonella, Gram-negative enteric microorganisms that form detectable colonies in and / or on said culture medium.

Embodiment 2

[0093] Embodiment 2 is the composition of embodiment 1, wherein the first differential indicator compound is selected from the group consisting of melibiose, 2-deoxy-D-ribose, mannitol, L-arabinose, galactose Alcohol, maltose, L-rhamnose, trehalose, D-xylose, sorbitol, and any combination of two or more of the above compounds.

Embodiment 3

[0094] Embodiment 3 is the composition of embodiment 1 or embodiment 2, further comprising a buffer.

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Abstract

A composition is provided for detecting a Salmonella microorganism in sample. The composition comprises at least one first selective agent that inhibits the growth of Gram-positive microorganisms, a first differential indicator system comprising at least one first differential indicator compound that is converted to a first detectable product by a Salmonella microorganism, and a second differential indicator system comprising a second differential indicator compound that is converted by urease enzyme activity to a second detectable product. Optionally, the composition may comprise a third differential indicator system comprising a third differential indicator compound that is converted by a beta-galactosidase enzyme activity to a third detectable product. Methods of using the composition to detect a Salmonella microorganism are also provided.

Description

[0001] Cross References to Related Applications [0002] This patent application claims the benefit of US Provisional Patent Application No. 61 / 580,849, filed December 28, 2011, which is hereby incorporated by reference in its entirety. Background technique [0003] The Enterobacteriaceae family includes a wide variety of metabolically distinct, facultatively anaerobic bacteria capable of fermenting sugars (eg, glucose) to lactic acid and / or other acidic end products. This family includes a variety of well-known human pathogens such as Escherichia coli, several subspecies of Salmonella, Yersinia pestis, several species of Klebsiella and several species of Shigella. [0004] The Salmonella genus includes two species, S. enterica and S. bongori, which include subspecies capable of causing disease in humans. Some pathogenic Salmonella species can be transmitted to humans through ingestion of contaminated food or drink. Due to the presence of relatively low numbers of Salmonel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12Q1/10
CPCG01N2333/255C12Q1/10C12Q1/045Y02A50/30
Inventor 克里斯蒂娜·A·宾斯费尔德帕特里克·A·马赫玛拉·S·切尔特亚当·J·斯塔内纳斯
Owner 3M INNOVATIVE PROPERTIES CO
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