Bacterium 16SrDNA (deoxyribonucleic acid) 500 fragment gene sequencing and dblast database analytical method

Abstract

A bacterium 16SrDNA (deoxyribonucleic acid) 500 fragment gene sequencing and dblast database analytical method comprises the following steps: step 1, extracting a bacterium DNA sample; step 2, performing bacterium 16SrDNA500 fragment gene amplification; step 3, purifying a PCR (polymerase chain reaction) product; step 4, cycling a sequencing reaction; step 5, a purifying a product of the sequencing reaction; step 6, preparing a capillary electrophoresis sample and performing capillary electrophoresis; and step 7.1, establishing gene testing quality analyzing and receiving standard. The method has the advantages of convenience, batch processing, accurate data and high stability.

Description

technical field [0001] The invention relates to a database analysis method, in particular to a bacterial 16SrDNA500 fragment gene sequencing and dblast database analysis method. Background technique [0002] Detect the bacteria in the environment and the diversity of bacteria, do DGGE, do high-throughput sequencing, and measure tens of thousands and hundreds of thousands of them. [0003] DGGE has obvious advantages when it is used to analyze environmental samples with simple flora structure, such as traditional fermented food, or changes in silage flora. [0004] The shortcomings of DGGE are very obvious, especially when used to analyze complex environmental samples. Especially in a very complex environment, there are hundreds of types of microorganisms. In contrast, the resolution of DGGE is very low, often separating 30 bands. Contents of the invention [0005] The purpose of the present invention is to provide a bacterial 16SrDNA500 fragment gene sequencing and dbla...

AI Technical Summary

Problems solved by technology

The resolution of DGGE is very low, often able to separate 30 bands