Unlock instant, AI-driven research and patent intelligence for your innovation.

A Method for Simultaneously Determining Mobility and Dielectric Mobility of Particles Using Distributed DC Electric Field

A technology of DC electric field and dielectric mobility, which is used in measurement devices, material analysis, material analysis by electromagnetic means, etc., can solve the cumbersome process, cannot reveal the information of particles such as cell charge and conductivity, and is difficult to meet the dielectric requirements. The need for property research and other issues to achieve the effect of optimal design

Active Publication Date: 2016-05-25
INST OF CHEM CHINESE ACAD OF SCI
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The latter device has a complex structure, difficult operation and control, and can be operated in a non-ordinary laboratory, and the measured data is related to the parameters of the instrument and device, so the versatility is poor
Different types of particles such as cells have wide differences in shape, size, surface charge, cell membrane morphology, cell wall properties, organelle composition, polarizability, dielectric properties, etc. The derivation and calculation based on theoretical models is cumbersome and difficult to meet various requirements. Particles such as cells and their dielectric properties in different physiological states need to be studied. At present, there is no experimental report on the measurement of dielectric mobility that is only related to the cells themselves.
In addition, the separate measurement of electrophoretic mobility and dielectric mobility cannot reveal the charge and conductivity information of particles such as cells at the same time point. It is necessary to develop a method that can simultaneously measure electrophoretic mobility and dielectric mobility to meet real-time dynamic research. The needs of processes such as changes in the physiological state of the cell itself and rapid interactions between cells and molecules

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Method for Simultaneously Determining Mobility and Dielectric Mobility of Particles Using Distributed DC Electric Field
  • A Method for Simultaneously Determining Mobility and Dielectric Mobility of Particles Using Distributed DC Electric Field
  • A Method for Simultaneously Determining Mobility and Dielectric Mobility of Particles Using Distributed DC Electric Field

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1. Simultaneous measurement of cell mobility and dielectric mobility by using "ten" cross channel

[0056] (1) Design and production of "ten" character cross passage structure device

[0057] Choose glass material to construct the cross channel, the channel size is as follows figure 2 As shown, the construction method of the channel is the same as that of glass microfluidic chip prepared by photolithography, including mask design, fabrication, UV exposure (15s), hydrofluoric acid etching (25°C, HF–HNO 3 –H 2 O solution etching for 20min), sealing, drilling and other steps.

[0058] (2) Chemical modification of the inner wall of the channel

[0059] Clean the channel with 1M NaOH, water, 1M HCl and water in sequence, then pour into γ-(methacryloyloxy)propyltrimethyloxysilane sol, after 30min, wash the channel with water, and then pour into the newly prepared prepolymerization solution, The prepolymerization solution consists of 2% (w / w) acrylamide, 10% (w / w)...

Embodiment 2

[0078] Example 2. Simultaneous determination of cell mobility and dielectric mobility using a spherical bubbling capillary

[0079] (1) Channel production:

[0080] Heated, melted and blown in a 100 micron inner diameter elastic glass capillary to form a bulb-like structure with a radius of 120 microns, the schematic diagram of the channel is as follows image 3 shown.

[0081] (2) The channel modification method is the same as in Example 1.

[0082] (3) Electrophoresis experiment

[0083] A 1cm×1cm liquid storage tank was made on polydimethylsiloxane, that is, a PDMS gasket, and a capillary port was inserted into the PDMS gasket to communicate with the buffer pool. Add PBS buffer solution (pH7.4) into the reservoir, and pour the solution into the capillary under capillary action. Add 5 μL of background electrolyte solution to the connected reservoirs, and adjust the liquid level of each reservoir until the cells are at rest within the field of view; apply a 50V DC voltage...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
radiusaaaaaaaaaa
electrical conductivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for simultaneously measuring mobility and dielectric mobility of particles by use of a distributed direct-current electric field. The method comprises the following steps of designing an electrophoresis channel structure so that an external direct-current electric field is distributed to cause the electrophoresis and dielectric electrophoresis coupled migration of the particles; recording the trajectory and speed of the coupled motion of the particles by use of a microimaging system; and finally, calculating the mobility and dielectric mobility of each particle by use of methods such as a speed vector decomposition method and a data fitting method. In addition, in combination with statistical analysis, the average mobility and average dielectric mobility of similar particles can be obtained. Based on measurement results of the method, the surface charge properties of particles such as cells and the characteristics of molecular recognition can be researched accurately; in terms of cells, the method is also capable of helping to predict the motion trajectory of the cells in processes of capturing, screening and gathering the cells, or to optimize the design of the electrophoresis system, in particular the channel shape; meanwhile, the invention also provides a new parameter for representing the classified particles.

Description

technical field [0001] The invention relates to a method for simultaneously measuring the mobility and dielectric mobility of particulate matter, in particular to a method for simultaneously measuring the mobility and dielectric mobility of particulate matter by using a distributed DC electric field. Background technique [0002] The mobility of particles such as cells can be used to characterize the surface charge of particles, which is an important physical parameter for studying the surface structure and function of particles such as cells. In the non-uniform electric field, in addition to the action of the DC electric field force, the cells and other particles also have the polarization or dielectric force of the non-uniform electric field, and then the phenomenon of dielectrophoresis (Pohl, H., Appl. Phys.1951 , 22, 869-871.). The size of the dielectric force is related to the conductivity and dielectric properties of the particles to be measured. Similar to the electr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447
Inventor 陈义朱秀珍
Owner INST OF CHEM CHINESE ACAD OF SCI