Application of a microbacterium strain and its effective algae-dissolving component in controlling cyanobacteria bloom

A microbacillus and algae-dissolving technology, which is applied in the field of environmental microorganisms, can solve the problems of high processing cost and secondary pollution, and achieve the effect of simple preparation method, short preparation cycle and significant lethal effect

Inactive Publication Date: 2016-09-07
SHANGHAI JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the high cost of physical and chemical methods in the prior art or the defects that easily cause secondary pollution, the technical problem to be solved by the present invention is to find high-efficiency algae-dissolving bacteria and to isolate and enrich the high-efficiency algae-dissolving activity produced by the metabolism of algae-dissolving bacteria. Substances for safe and efficient control of cyanobacterial blooms

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  • Application of a microbacterium strain and its effective algae-dissolving component in controlling cyanobacteria bloom
  • Application of a microbacterium strain and its effective algae-dissolving component in controlling cyanobacteria bloom
  • Application of a microbacterium strain and its effective algae-dissolving component in controlling cyanobacteria bloom

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Embodiment 1

[0034] The screening of embodiment 1 algae-lytic bacteria

[0035] Add 10mL of natural water samples collected from the Meiliang Bay waters of Taihu Lake to 90mL of algae liquid of Microcystis aeruginosa 9110 in the logarithmic phase, take the yellow algae liquid two days later, apply the gradient dilution method to the beef extract peptone medium agar plate, and cultivate at 28°C After 24 hours, take a plate with a moderate colony density, and select different strains according to the different colony shapes.

[0036] The screened strains were inoculated into 10 mL of beef extract peptone medium, cultured at 28°C and 220 rpm for 24 hours, and 10 mL of cultured bacterial liquid was added to 90 mL of algae liquid of Microcystis aeruginosa in logarithmic phase. In addition, 10 mL of sterilized beef extract peptone medium was also added to 90 mL of algae liquid as a control. All the algae fluids of the experimental group and the control group were cultured in the light incubator...

Embodiment 2

[0040] The identification of embodiment 2 microbacteria GLY-3109 bacterial strain

[0041] The strain GLY-3109 was identified by morphological observation, staining, physiological and biochemical reactions, and 16srRNA gene sequence analysis. flagellar movement. Facultative anaerobic. On nutrient agar, the colonies are flat, light orange, and the pigment does not diffuse. Basophilic, able to grow at pH 6.5-11.5. Acids can be produced from glucose, sucrose, galactose, and some other sugars, the main products being lactic, acetic, and formic acids. The strain was positive for contact enzyme and negative for oxidase. Can reduce nitrates, hydrolyze gelatin, starch and casein. The optimum growth temperature is 37°C. After 16srRNA gene sequence analysis and homology comparison, it was known that the strain had 99% homology with a certain Exobacterium strain in GenBank, so it was identified as an Exobacterium genus and named Exobacterium GLY-3109 strain.

Embodiment 3

[0042] The preparation method of embodiment 3 microbacterium GLY-3109 fermented liquid and ethyl acetate extract thereof

[0043] Microbacterium GLY-3109 was inoculated in sterilized beef extract peptone medium with pH 7.0 according to 1% inoculation amount, and cultured on a shaker at 220 rpm at 28° C. for 48 hours to obtain a fermentation broth containing Microbacterium GLY-3109. Add ethyl acetate into the fermentation broth at a ratio of 1:1, put it into a shaker and vibrate for 24 hours, and separate the upper layer solution, that is, the ethyl acetate extract. The ethyl acetate was evaporated to dryness, dissolved in water, and filtered through a microporous membrane with a pore size of 0.22 μm for further purification of metabolites.

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Abstract

The invention discloses the application of a microbacterium strain and its effective algae-dissolving component 3-benzyl-2,5-diketopiperazine in controlling cyanobacteria bloom. A strain of Exiguobacterium sp. GLY‑3109 with significant algicidal activity was isolated from the water body of Taihu Lake, the preservation number is CGMCC No.8980, and its effective algicidal component was isolated and purified from its metabolites 3‑benzyl‑2,5‑diketopiperazine, the LD50 of this alginolytic substance to Microcystis aeruginosa 9110 is 4.8 μg / mL. It can be used in the research and development and production of new biological algicides, and finally applied to the control of cyanobacteria blooms in lakes.

Description

technical field [0001] The present invention relates to the field of environmental microorganisms, in particular to a strain of Exiguobacterium sp. GLY-3109 with algae-dissolving activity and the secreted effective algae-dissolving component 3-benzyl-2,5-diketopiperazine and its use in cyanobacteria water Application in Hua control. Background technique [0002] In oceans, lakes and reservoirs, cyanobacteria can form algae blooms and red tides, thereby affecting and changing the physical and chemical properties of water, and can directly and indirectly cause a large number of algae-eating animals to die. Especially for drinking water production with lake water and reservoir water as water sources, the harm of algae is more serious. Therefore, it is very necessary to explore effective ways to control the cyanobacterial biomass and inhibit the occurrence of cyanobacterial blooms. [0003] At present, there are some water bloom control methods, such as physical methods (mecha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F1/50C12R1/01
Inventor 杨虹耿梦馨郭星亮
Owner SHANGHAI JIAOTONG UNIV
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