Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Exiguobacterium sp. GLY-3109 and use of Exiguobacterium sp. GLY-3109 and its active algicidal component in control of cyanobacterial bloom

A technology of microbacteria and cyanobacteria blooms, applied in the field of environmental microorganisms, can solve the problems of high treatment cost and secondary pollution, and achieve the effect of simple preparation method, short preparation cycle and significant lethal effect

Inactive Publication Date: 2014-11-05
SHANGHAI JIAO TONG UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the high cost of physical and chemical methods in the prior art or the defects that easily cause secondary pollution, the technical problem to be solved by the present invention is to find high-efficiency algae-dissolving bacteria and to isolate and enrich the high-efficiency algae-dissolving activity produced by the metabolism of algae-dissolving bacteria. Substances for safe and efficient control of cyanobacterial blooms

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Exiguobacterium sp. GLY-3109 and use of Exiguobacterium sp. GLY-3109 and its active algicidal component in control of cyanobacterial bloom
  • Exiguobacterium sp. GLY-3109 and use of Exiguobacterium sp. GLY-3109 and its active algicidal component in control of cyanobacterial bloom
  • Exiguobacterium sp. GLY-3109 and use of Exiguobacterium sp. GLY-3109 and its active algicidal component in control of cyanobacterial bloom

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The screening of embodiment 1 algae-lytic bacteria

[0035] Add 10mL of natural water samples collected from the Meiliang Bay waters of Taihu Lake to 90mL of algae liquid of Microcystis aeruginosa 9110 in the logarithmic phase, take the yellow algal liquid two days later, apply the gradient dilution method to the beef extract peptone medium agar plate, and cultivate at 28°C After 24 hours, take a plate with a moderate colony density, and select different strains according to the different colony shapes.

[0036] The screened strains were inoculated into 10 mL of beef extract peptone medium, cultured at 28°C and 220 rpm for 24 hours, and 10 mL of cultured bacterial liquid was added to 90 mL of algae liquid of Microcystis aeruginosa in logarithmic phase. In addition, 10 mL of sterilized beef extract peptone medium was also added to 90 mL of algae liquid as a control. All the algae fluids of the experimental group and the control group were cultured in the light incubator...

Embodiment 2

[0040] The identification of embodiment 2 microbacteria GLY-3109 bacterial strain

[0041] The strain GLY-3109 was identified by morphological observation, staining, physiological and biochemical reactions, and 16srRNA gene sequence analysis. flagellar movement. Facultative anaerobic. On nutrient agar, the colonies are flat, light orange, and the pigment does not diffuse. Basophilic, able to grow at pH 6.5-11.5. Acids can be produced from glucose, sucrose, galactose, and some other sugars, the main products being lactic, acetic, and formic acids. The strain was positive for contact enzyme and negative for oxidase. Can reduce nitrates, hydrolyze gelatin, starch and casein. The optimum growth temperature is 37°C. After 16srRNA gene sequence analysis and homology comparison, it was known that the strain had 99% homology with a certain Exobacterium strain in GenBank, so it was identified as an Exobacterium genus and named Exobacterium GLY-3109 strain.

Embodiment 3

[0042] The preparation method of embodiment 3 microbacterium GLY-3109 fermented liquid and ethyl acetate extract thereof

[0043] Microbacterium GLY-3109 was inoculated in sterilized beef extract peptone medium with pH 7.0 according to 1% inoculation amount, and cultured on a shaker at 220 rpm at 28° C. for 48 hours to obtain a fermentation broth containing Microbacterium GLY-3109. Add ethyl acetate into the fermentation broth at a ratio of 1:1, put it into a shaker and vibrate for 24 hours, and separate the upper layer solution, that is, the ethyl acetate extract. The ethyl acetate was evaporated to dryness, dissolved in water, and filtered through a microporous membrane with a pore size of 0.22 μm for further purification of metabolites.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Exiguobacterium sp. GLY-3109 and a use of the Exiguobacterium sp. GLY-3109 and its active algicidal component 3-benzyl-2,5-diketopiperazine in control of cyanobacterial bloom. The Exiguobacterium sp. GLY-3109 separated from water of Taihu Lake has substantial algicidal activity and an accession number of CGMCC No. 8980. The active algicidal component 3-benzyl-2,5-diketopiperazine is separated from a metabolite of the Exiguobacterium sp. GLY-3109, is purified and is identified. The active algicidal component has microcystis aeruginosa lethal dose 50 (LD50) of 4.8 micrograms per milliliter. The Exiguobacterium sp. GLY-3109 can be used for research, development and production of a novel biological algicide and can be finally used for control of cyanobacterial bloom.

Description

technical field [0001] The present invention relates to the field of environmental microorganisms, in particular to a strain of Exiguobacterium sp. GLY-3109 with algae-dissolving activity and the secreted effective algae-dissolving component 3-benzyl-2,5-diketopiperazine and its use in cyanobacteria water The application in Hua control. Background technique [0002] In oceans, lakes and reservoirs, cyanobacteria can form algae blooms and red tides, thereby affecting and changing the physical and chemical properties of water, and can directly and indirectly cause a large number of algae-eating animals to die. Especially for drinking water production with lake water and reservoir water as water sources, the harm of algae is more serious. Therefore, it is very necessary to explore effective ways to control the cyanobacterial biomass and inhibit the occurrence of cyanobacterial blooms. [0003] At present, there are some water bloom control methods, such as physical methods (m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F1/50C12R1/01
Inventor 杨虹耿梦馨郭星亮
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com