A kind of fermentation medium and fermentation method for improving the yield of enramycin
The technology of fermentation medium and enramycin, which is applied in the field of microbial fermentation, can solve the problems of high production cost of susceptible bacteria, enramycin, and unsatisfactory fermentation results, and achieves the effect of improving the fermentation unit.
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Embodiment 1
[0027] Inoculate the experimental bacteria on a solid slant medium under aseptic conditions, and culture at a constant temperature of 28°C for 7 days; cultivate the fungicide Streptomyces in the seed medium to obtain a seed liquid, and the seed liquid is 8%-10% (v / v) inoculum amount is inoculated in a 50L fermenter with a liquid content of 35L of fermentation medium for fermentation, the fermentation temperature is 28°C, and the fermentation period is 8 days. Samples were taken (HPLC) after fermentation to determine that the titer of enramycin could reach 9133 μg / mL.
[0028] Wherein, the composition of the solid slant medium is: glucose 10g / L, tryptone 1g / L, agar 22g / L, yeast powder 1g / L.
[0029] The composition of described seed culture medium is: corn meal 40g / L, corn steep liquor 28g / L, cottonseed meal meal 5g / L, corn gluten meal 5g / L, ammonium sulfate 3g / L, ferrous sulfate 0.36g / L, phosphoric acid Potassium dihydrogen 1.25g / L, calcium carbonate 22g / L, foam enemy 0.6g / L,...
Embodiment 2
[0032] The experimental strains were inoculated on a solid slant medium under sterile conditions, and cultured at a constant temperature of 28°C for 7 days; the seed liquid was inoculated in the fermentation medium with an inoculation amount of 8%-10%, the fermentation temperature was 28°C, and the fermentation period was 10 days . Samples were taken (HPLC) after fermentation to determine that the titer of enramycin could reach 8517 μg / mL.
[0033] Described solid slant culture medium and seed culture medium are with embodiment 1;
[0034] The composition of described fermentation medium: glucose 40g / L, cornstarch 15g / L, soybean meal powder 21g / L, cottonseed cake powder 10g / L, corn steep liquor hydrolyzate 25g / L, yeast extract powder 1g / L, NaCl15g / L , NH 4 Cl 5g / L, CaCO 3 20g / L, foam enemy 0.6g / L, adjust the pH to 7.1 with 20% liquid alkali, and finally put in calcium carbonate.
Embodiment 3
[0036] The experimental strains were inoculated on a solid slant medium under sterile conditions, and cultured at a constant temperature of 28°C for 7 days; the seed liquid was inoculated in the fermentation medium with an inoculation amount of 8%-10%, the fermentation temperature was 28°C, and the fermentation cycle was 9 days . Sampling (HPLC) was performed after fermentation, and the titer of enramycin could reach 9048 μg / mL.
[0037] Described solid slant culture medium and seed culture medium are with embodiment 1;
[0038] The composition of described fermentation medium: glucose 40g / L, cornstarch 25g / L, soybean meal powder 21g / L, cottonseed cake powder 10g / L, corn steep liquor hydrolyzate 20g / L, yeast extract powder 1g / L, NaCl15g / L , NH4 Cl 5g / L, CaCO 3 20g / L, foam enemy 0.6g / L, adjust the pH to 7.0 with 20% liquid alkali, and finally put in calcium carbonate.
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