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Preparation method for double-functional mark photo-electrochemical sensor and application

A photoelectrochemical, dual-function technology, which is applied in scientific instruments, instruments, and material analysis through electromagnetic means, can solve the problems of low photoelectric conversion efficiency in the visible light region, and achieve the effect of improving sensitivity and accurate detection

Inactive Publication Date: 2014-11-05
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the disadvantage is that TiO2 has a wide band width (3.2 eV), so that it can only be effectively excited in the ultraviolet region, and the photoelectric conversion efficiency in the visible region is extremely low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation method of bifunctional labeled photoelectrochemical sensor

[0043] (1) Pretreatment of the ITO electrode: cut the ITO glass to a size of 3 cm × 0.5 cm, ultrasonically clean it with detergent, acetone, ethanol, and ultrapure water for 30 min, and finally dry the glass with nitrogen, and wrap it with insulating tape. One end of the ITO electrode leaves a 0.5 cm × 0.5 cm area for modifying biomolecules;

[0044] (2) Soak the treated ITO electrode in a methanol solution of 1% 3-aminopropyltriethoxysilane APTES in the dark for 10 hours, and rinse the electrode with water to remove unbound APTES molecules ;

[0045] (3) Take 10 μL of freshly prepared gold nano-sol and add it dropwise on the surface of the electrode to bind the primary antibody molecule of CA125;

[0046] (4) Add 6 μL, 8 μg / mL CA125 primary antibody solution dropwise to the electrode, incubate in a constant temperature refrigerator at 4 °C for 2 h, and rinse the surface of the electr...

Embodiment 2

[0050] Example 2 Preparation method of bifunctional labeled photoelectrochemical sensor

[0051] (1) Pretreatment of the ITO electrode: cut the ITO glass to a size of 3 cm × 0.5 cm, ultrasonically clean it with detergent, acetone, ethanol, and ultrapure water for 30 min, and finally dry the glass with nitrogen, and wrap it with insulating tape. One end of the ITO electrode leaves a 0.5 cm × 0.5 cm area for modifying biomolecules;

[0052] (2) Soak the treated ITO electrode in a methanol solution with a volume fraction of 2% 3-aminopropyltriethoxysilane APTES for 12 h in the dark, and rinse the electrode with water to remove unbound APTES molecular;

[0053] (3) Take 10 μL of freshly prepared gold nano-sol and add it dropwise on the surface of the electrode to bind the primary antibody molecule of CA125;

[0054] (4) Add 6 μL, 10 μg / mL CA125 primary antibody solution dropwise to the electrode, incubate in a constant temperature refrigerator at 4 °C for 2 h, and rinse the su...

Embodiment 3

[0058] Example 3 Preparation method of bifunctional labeled photoelectrochemical sensor

[0059] (1) Pretreatment of the ITO electrode: cut the ITO glass to a size of 3 cm × 0.5 cm, ultrasonically clean it with detergent, acetone, ethanol, and ultrapure water for 30 min, and finally dry the glass with nitrogen, and wrap it with insulating tape. One end of the ITO electrode leaves a 0.5 cm × 0.5 cm area for modifying biomolecules;

[0060] (2) Soak the treated ITO electrode in a methanol solution of 3-aminopropyltriethoxysilane APTES with a volume fraction of 3%, soak in the dark for 14 h, and rinse the electrode with water to remove unbound APTES molecular;

[0061] (3) Take 10 μL of freshly prepared gold nano-sol and add it dropwise on the surface of the electrode to bind the primary antibody molecule of CA125;

[0062] (4) Add 6 μL, 12 μg / mL CA125 primary antibody solution dropwise to the electrode, incubate in a constant temperature refrigerator at 4 °C for 2 h, and rin...

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Abstract

The invention provides a preparation method for a double-functional mark photo-electrochemical sensor and application and belongs to the technical fields of nano-functional materials, clinical analysis, bio-sensing and electrochemistry. A TiO2-CdSe semiconductor compound is prepared based on a ligand principle between TiO2 and carboxyl; a remarkably-enhanced photoelectric conversion effect of the prepared TiO2-CdSe semiconductor compound is displayed on a visible region; a photocurrent value is 10 times as much as that of a single TiO2 photocurrent value; the TiO2-CdSe semiconductor compound has large specific surface area and good biocompatibility and is used for marking a second antibody (Ab2) of CA125 so that a TiO2-CdSe-Ab2 hatched matter is prepared. The difference between the double-functional mark photo-electrochemical sensor and other electrochemical or photo-induced electrochemical sensors is as follows: the TiO2-CdSe semiconductor compound can be used for not only carrying out photoelectric conversion and but also generating Cd2<+>, so that an immunosensor taking the TiO2-CdSe semiconductor compound as a second antibody marker can adopt an electrochemical analysis technology and a photo-induced electrochemical analysis technology to rapidly, sensitively and accurately detect the CA125. The method has certain guiding significance and application value on early diagnosis of cancers.

Description

technical field [0001] The invention belongs to the technical field of functionalized nanomaterials, immune analysis and biosensing, and specifically relates to a bifunctional TiO 2 Preparation and application of sandwich immunosensor constructed with / CdSe semiconductor label. Background technique [0002] Because of the energy level difference between the energy band and the valence band of the semiconductor material, it will produce electronic transitions under the excitation of light and other energy, thus presenting a semiconducting state between a conductor and an insulator. [0003] TiO 2 It is a semiconductor material often used in photocatalysis, solar cells and fuel cells. TiO 2 It has the characteristics of easy batch synthesis and no pollution to the environment. Compared to bulk TiO 2 In terms of nanoscale TiO 2 It has the advantages of large specific surface area, good bio-affinity and easy functionalization. But the disadvantage is that TiO 2 The energ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/531G01N27/327
CPCG01N33/54366G01N33/57488
Inventor 魏琴高丕成张勇马洪敏庞雪辉胡丽华
Owner UNIV OF JINAN
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