Method for obtaining anthurium haplobionts

A haploid and plant technology, applied in the field of genetic breeding of flower seedlings, can solve the problems of slow process, shortened breeding period, unstable offspring, etc., achieve obvious effects, increase yield rate, increase anther expansion rate and effective callus The effect of tissue induction rate

Active Publication Date: 2014-11-12
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a method that can overcome the separation of hybrids, shorten the breeding period, and obtain high-purity Anthurium haploid bre...

Method used

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  • Method for obtaining anthurium haplobionts

Examples

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Effect test

Embodiment 1

[0025] Embodiment 1: (Method 1 for obtaining anthurium variety 'Alabama (Alabamb)' haploid plant)

[0026] The method proceeds as follows:

[0027] (1) Preparation of medium: including medium for each stage of anther culture, among which,

[0028] 1) Induction medium: 1 / 2MS basic medium supplemented with 0.5mg / L 2,4-D, 0.5mg / L 6-BA, 30g / L sucrose, 20g / L glucose, 6g / L agar powder, pH sterilized former 5.8;

[0029] 2) Proliferation medium: MS basic medium added with 1.0mg / L 6-BA, 30g / L sucrose, pH 5.8 before sterilization;

[0030] 3) Differentiation medium: MS basic medium added with 1.0mg / L 6-BA, 30g / L sucrose, pH 5.8 before sterilization;

[0031] 4) Rooting medium: 1 / 2MS basic medium with 1g / L activated carbon, 20g / L sucrose, pH 5.8 before sterilization;

[0032] (2) Selection of inflorescences, low-temperature pretreatment and disinfection: take the anthurium variety 'Alabama (Alabamb)' as the test material, and after the spathe inflorescences are extracted, select th...

Embodiment 2

[0041] Embodiment 2: (method 2 for obtaining haploid plants of anthurium variety 'Alabama (Alabamb)'):

[0042] In this example, the 2,4-D added to the induction medium formula in step (1) is 1.5 mg / L, the 6-BA is 1 mg / L, and the sucrose is 45 g / L; Treat for 36 hours; in step (8), take 0.4 cm white young root tips, treat with 0.002 mol / L 8-hydroxyquinoline for 2.5 hours, fix with Carnot’s fixative for 20 hours, and acidify with 0.2mol / L HCl for 17 minutes The rest of the step process is the same as in Example 1, the result: the sterile anther rate obtained in step (3) is 59.42%, the anther expansion rate is 75.50%, the effective callus induction rate is 29.00%, and the haploid in step (8) The proportion of seedlings is about 40%.

[0043]

Embodiment 3

[0044] Embodiment 3: (method 3 for obtaining haploid plants of anthurium variety 'Alabama (Alabamb)'):

[0045] In this example, 2,4-D, 1.5 mg / L 6-BA, and 55 g / L sucrose were added to the induction medium formula in step (1); Under treatment for 48 hours; in step (8), 0.5 cm white young root tips were taken, treated with 0.002 mol / L 8-hydroxyquinoline for 2.5 hours, fixed with Carnot’s fixative for 22 hours, and acidified with 0.2 mol / L HCl for 19 hours. min; the rest of the step process is the same as in Example 1, the result: the aseptic anther rate obtained in step (3) is 43.33%, the anther expansion rate is 60.82%, and the effective callus induction rate is 19.21%. The proportion of body seedlings is about 30%.

[0046]

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Abstract

The invention discloses a method for obtaining anthurium haplobionts and belongs to the technical field of genetic breeding of flowers and plants. The method includes the steps that (1) culture mediums are prepared; (2) inflorescences are selected, pretreated at low temperature and disinfected; (3) callus is induced; (4) proliferation culture is performed; (5) differentiation culture is performed; (6) rooting culture is performed; (7) seedlings are transplanted and hardened; (8) chromosomes are identified; (9) haploid propagation is performed. After the anthurium inflorescences are pretreated at the low temperature from 5 DEG C to 8 DEG C for 24 h to 72 h, the acquisition rate of sterile anthers and the induction rate of effective callus can be remarkably increased, and thus anthurium haplobionts can be effectively obtained; the operation method is easy and convenient to perform, the anthurium haplobionts can be used for further breeding a new variety of anthurium as the breeding material, and the breeding cycle is shortened.

Description

technical field [0001] The invention relates to the technical field of genetic breeding of flower seedlings, in particular to a method for obtaining anthurium haploid plants. Background technique [0002] Anthurium ( A. andraeanum ) Also known as Anthurium, Anthurium, etc., Araceae Anthurium, native to Colombia, is a perennial, evergreen, herbaceous plant that blooms in four seasons and can be used for viewing flowers and leaves. It can be divided into two categories: cut flowers and potted plants. Due to its unique flower shape, bright and colorful colors, natural shade tolerance, and long viewing period, it has a large consumption in domestic and foreign markets, ranking second only to orchids in the global tropical flower trade. [0003] Haploid refers to individuals with gamete chromosomes (n). Generally, anthers and pollen are used as exocytes for tissue culture, and plants are produced from small spores through callus or embryoid bodies, or parthenogenesis, Plant...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 田丹青潘晓韵葛亚英潘刚敏沈晓岚刘建新金亮
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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