Device and method for full-automatically sorting circulating tumor cells on micro-fluidic chip

Abstract

The invention discloses a device and method for full-automatically sorting circulating tumor cells on a micro-fluidic chip. The device mainly comprises the PDMS micro-fluidic chip and an electromagnetism microvalve structure. The PDMS micro-fluidic chip comprises liquid storage holes (A, B, C, D, E and F), a detecting channel, a main channel, a first focusing channel, a second focusing channel, a sample outlet channel, a target cell collecting channel and an electromagnetic sorting channel. When the circulating tumor cells pass through the detecting channel, produced voltage signals are detected and sent to an NI collecting card and a processing terminal, the processing terminal sends out a voltage signal through the NI collecting card so that an electromagnetic relay can be switched off, the electromagnetic microvalve structure presses a PDMS layer below the electromagnetic microvalve structure, the PDMS layer deforms, a part of liquid is drained out of the liquid storage hole E, and the circulating tumor cells are pushed to flow to the target cell collecting channel. According to the device, the embedded type electromagnetic microvalve structure is used for providing driving force for sorting, electroosmosis driving and electroosmosis focusing are adopted on samples, and the circulating tumor cells can be detected and sorted in a full automatic mode.

Description

technical field [0001] The invention relates to the field of particle sorting, in particular to a device and method for automatically sorting circulating tumor cells from whole blood on a microfluidic chip. Background technique [0002] In the fields of biology and medicine, it is often necessary to sort out specific target cells from more complex samples. For example, detecting, sorting and counting the number of circulating tumor cells (CTC, circulating tumor cells) in the blood of cancer patients is becoming more and more important in the diagnosis and treatment of cancer. [0003] The traditional methods for sorting circulating tumor cells from whole blood mainly include density gradient centrifugation and immunomagnetic bead method. Currently, the most widely used method is immunomagnetic bead method. The centrifugation method requires a large amount of samples and takes a long time, requiring the use of large-scale equipment; the immunomagnetic bead method must first ...

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Problems solved by technology

However, there are various deficiencies in this method. For example, the direct current dielectrophoresis method needs to apply a high electric field strength, which is very easy to cause cell lysis; the hydrodynamic method requires precise control of various pressures, and can only work effectively for a short period of time. After that, it is necessary to use a microscope to adjust the liquid level; while the method of fluorescence sorting requires fluorescent labeling of cells and the construction of a complex optical detection system

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